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Citations for Polysciences :
1 - 50 of 886 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Plant Biology 2021Quote: ... post-stained 1% aqueous uranyl acetate (Polysciences) and Sato’s triple lead (sodium citrate ...
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bioRxiv - Biophysics 2022Quote: ... the excess solvent was blotted and 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was added on the grid ...
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bioRxiv - Cell Biology 2022Quote: ... After 6 h of LPS treatment 16% Formaldehyde (18814-20, Polysciences Inc.) was added to the medium in the dish to a concentration of 1% and incubated (gently mixing ...
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bioRxiv - Biophysics 2023Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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bioRxiv - Biochemistry 2020Quote: ... samples were stained with 1% aqueous uranyl acetate (Polysciences, Inc. ...
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bioRxiv - Microbiology 2023Quote: ... samples were stained with 1% aqueous uranyl acetate (Polysciences, Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Plant Biology 2020Quote: ... Grids were post-stained with 1% aqueous uranyl acetate (Polysciences) and Sato’s triple lead (Sato ...
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bioRxiv - Plant Biology 2021Quote: ... Grids were post-stained with 1% aqueous uranyl acetate (Polysciences) and Sato’s triple lead (sodium citrate ...
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bioRxiv - Biochemistry 2023Quote: ... and staining with 1% uranyl acetate (Polysciences, Inc, Warrington, PA).
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 1% tannic acid and 1% uranyl acetate (Polysciences Inc. ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Genetics 2019Quote: ... at a weight ratio of 4:3:2 using 54 µL PEI (Polysciences, 24765-1, 1 µg/µl). We changed the medium after 4-6 hours of incubation at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Microbiology 2021Quote: ... and stained with 1% aqueous uranyl acetate (Polysciences, Inc, Warrington, PA). Stained grids were imaged under Talos120C transmission electron microscope (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... All reagents were from Sigma-Aldrich except uranyl acetate (Polysciences, Philadelphia).
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bioRxiv - Cell Biology 2023Quote: ... 0.1% uranyl acetate (Polysciences Inc) and 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... followed by fixation using 4% paraformaldehyde (18814-20, Polysciences Inc.) in 1X PBS for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were stained with 1 mL of 2% uranyl acetate (Polysciences #21447-25) in water for 30 min at room temperature and then briefly washed with 50% ethanol (Pharmco #111000200 ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% uranyl acetate (Polysciences, USA). Freeze-substitution was done using an automatic freeze substitution machine (EM AFS ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was fixed with 4% paraformaldehyde (Polysciences, Cat# 18814-20) for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... and 0.1% uranyl acetate (Polysciences; Warrington, PA) in 2% H2O and 98% acetone in an FS-8500 freeze substitution system ...
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bioRxiv - Cancer Biology 2022Quote: ... The circulating hemocytes were fixed with 4% paraformaldehyde (Polysciences, Cat# 18814-20) and centrifuged for 5 min at 1000 rpm before incubation for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2019Quote: Flies of both genders were raised as described above and larvae from second instar to pre-pupal stages collected and dissected directly into fresh 4% EM grade paraformaldehyde solution (from a 16% stock. Polysciences) in PBS with 0.3% TritonX-100 then incubated for 25 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.1% (w/v) uranyl acetate (Polysciences, USA). Freeze substitution was performed in a Leica AFS (Leica Microsystems ...
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bioRxiv - Neuroscience 2020Quote: ... and negatively stained with 0.5% uranyl acetate (Polysciences). The grids were imaged in a FEI Tecnai F20 electron microscope (Eindhoven ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% (w/v) uranyl acetate (Polysciences, USA). Freeze substitution was performed in either a Leica AFS or a Lecia AFS II (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 3 time in 1xPBS before being mounted with Aqua Polymount (Polysciences, 18606-20). The following primary antibodies were used ...
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bioRxiv - Microbiology 2024Quote: ... and NiV-M-GFP at a 9:1:5 ratio by using polyethylenimine (PEI) at 1 mg/ml (Polysciences, 23966-100). At 48hrs posttransfection ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... consisting of 0.1 % (w/v) uranyl acetate (Polysciences, USA) and 1 % (w/v ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 μl polyethylenimine (PEI; 1 mg/ml) (Polysciences) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... were stained with 2% uranyl acetate (Polysciences; Cat#6159-44-0) and lead citrate (Polysciences ...
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bioRxiv - Cell Biology 2023Quote: ... Ultrathin sections (60-80 nm) were stained with uranyl acetate (Polysciences) and lead citrate (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...