Labshake search
Citations for Polysciences :
201 - 250 of 839 citations for 6' ETHYL SPIRO 1 3 DIOXANE 2 3' INDOLIN 2' ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Developmental Biology 2021Quote: ... were stained with 2% uranyl acetate (Polysciences; Cat#6159-44-0) and lead citrate (Polysciences ...
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bioRxiv - Molecular Biology 2019Quote: ... Polyethylenimine (PEI) 25kD Linear Form was obtained from Polysciences (#23966-2). All other materials were of reagent grade.
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bioRxiv - Cancer Biology 2022Quote: ... and pLP/VSVG (2 µg) (clontech) using Polyethylenimine (PEI) Max (Polysciences). Virus containing supernatant was collected 48 h after transfection and cleared through a 0.45-µm PDF filter ...
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bioRxiv - Neuroscience 2021Quote: ... or 5 µl of 2% Fast-Blue (Polysciences, Warrington, PA, USA). Tracer leakage was prevented by sealing the reservoir around the nerve with Vaseline (Vaselinum album ...
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bioRxiv - Neuroscience 2020Quote: ... and 48 μl of 2 mg/mL polyethyleneimine (PEI; Polysciences, 24765) were mixed and transfected into Lenti-X 293T (Clontech ...
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bioRxiv - Physiology 2021Quote: ... Cells were transfected with Polyethylenimine-PEI Transfection Reagent (Polysciences, 23966-2) carrying the LC3-eGFP plasmid (60 ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfection of packaging cells was performed using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... using Polyethylenimine according to the manufacturer’s instructions (PEI, Polysciences #24765-2).
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bioRxiv - Cancer Biology 2023Quote: ... were transfected into HEK293FT cells using 2 μg of Polyethylenimine (Polysciences). Lentiviral particle containing media was filtered using 0.45μm cellulose acetate syringe filter and used for transduction ...
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bioRxiv - Cell Biology 2023Quote: NGN2-iN lentiviruses were produced by polyethylenimine (Polysciences, PEI, 23966-2,) in HEK 293T cells (ATCC ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 minutes and mounted using AquaPolymount (Polysciences, Warrington, USA). Images were obtained on a Confocal and STED FACILITY line microscope (Abberior Instruments ...
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bioRxiv - Biochemistry 2020Quote: Plasmids (500 ng DNA per ml culture) and polyethylenimine (MW 25,000; Polysciences; 3 µg per ml culture) were mixed with OptiMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Neuroscience 2020Quote: ... sections were subsequently washed with PBS 1X (3 × 10 min) and mounted in Aqua/polymount (Polysciences 18606).
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 0.4 mg each of TCR and CD3 DNA were mixed with 3 mg of PEI MAX (Polysciences) and added to 0.8 L of HEK293F cells grown in suspension in FreeStyle 293 media (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: Slices were washed 3 times for 10 minutes in PBS and mounted with Aqua-Poly/Mount (Polysciences). Images were taken using a Zeiss LSM780 confocal microscope and images were processed using ImageJ software ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Finally sections were subsequently washed with PBS (3 x 10 min) and mounted in Aqua/polymount (Polysciences, 18606).
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... pNL4-3 and pNL4-3dNef were transfected into HEK293T cells using polyethylenimine (PEI) transfection reagents (Polysciences, PA, USA). At 48 h post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3μl of 1.25% (w/v) polystyrene microspheres (Polybead Microspheres 0.05μm, Polysciences) and 0.2mM levamisole (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently transferred to 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... were produced as previously described using a PEI (polyethylenimine, Polysciences, 24765-2) transfection protocol followed by iodixanol gradient purification ...
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bioRxiv - Molecular Biology 2019Quote: Overexpression of vectors was performed using Polyethylenimine MAX 4000 (Polysciences, 24885-2) with a DNA:PEI ratio of 1:10 ...
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bioRxiv - Biophysics 2021Quote: ... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... and 48 μL of Polyethylenimine ‘Max’ MW 40,000 Da (Polysciences; 24765-2) per plate ...
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bioRxiv - Physiology 2022Quote: ... C3H10T1/2 cells were transfected with Transporter 5® Transfection Reagent (Polysciences) following manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... with 2 μl of 100 nm polystyrene beads (Cat# 00876-15, Polysciences) and 2 μl of levamisole (from 500 μM stock ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...