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Citations for Polysciences :
1 - 50 of 271 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... young adult animals were mounted onto 1-1.5% of agar pad with 3-5 µL of latex beads (Polybeads, 0.2 µm, PolySciences) to facilitate body movement and stabilize the position of field of view22 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the pNL4-3-inGluc and S constructs in a 2:1 ratio using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) in order to generate viral particles ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... four L square glass bottles each containing 200 mL of suspension culture at ∼2.5 × 106 cell/mL were transfected using 1 µg/mL DNA and 3 µg/mL polyethylene ‘Max’ 40 kDa (Polysciences, Warrington, PA, #24765). The pre-premixing of DNA and PEI-Max was incubated for 20 min at room temperature before added to the bottles ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transporter 5™ reagent (Polysciences, Inc, Cat #26008-5) was used to transfect parental HEK293T/17 cells ...
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bioRxiv - Biochemistry 2023Quote: ... -MCM9 M45E or -GFP control (Transporter 5, Polysciences 26008-5) and harvested 3 days later for HA-immunoprecipitation as previously described39 ...
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bioRxiv - Biophysics 2021Quote: ... carboxylated 3 µm silica beads (Polysciences) were coated with a mixture of biotinylated pentameric FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs70 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 µm polystyrene beads (Polysciences) suspended in 1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... with modified pCW57.1 target vector in a ratio of 5 : 1 : 5 : 5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966). Three days post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Fast Blue (5%, Polysciences) or rAAV-hSyn-EGFP (1012vp/ml ...
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bioRxiv - Microbiology 2024Quote: ... a Tat-encoding plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1:3 ratio using polyethyleneimine (PEI, Polysciences). After 8 hours ...
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: For HIV-1 virus growth assay pNL4-3 and its mutated versions pNL4-3 TC-1G and pNL4-3 TC-3G were transfected into freshly seeded 293T cells using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfection was done with 3 μg polyethylenimine (Polysciences) per 3.5 cm dish 1 day after plating ...
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bioRxiv - Immunology 2021Quote: ... fixed 18 hrs later with 3% formaldehyde (Polysciences) in PBS ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... using polyethylenimine (PEI, 3 μg/ml, Polysciences, 23966), with 1 μg of the indicated plasmids ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 3 μl polyethyleneimine (PEI, Polysciences), 1 μg plasmid DNA (750 ng ABEs or A&C-BEs expression plasmid and 250 ng sgRNA expression plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 3 μl polyethyleneimine (PEI, Polysciences), 850 ng plasmid DNA (250 ng SpCas9 sgRNA plasmid ...
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bioRxiv - Immunology 2024Quote: ... 3 μl of PEI-Max transfection reagent (Polysciences) (1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... or Aquamount (#18606-5, Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Biophysics 2024Quote: 5 µm fluorescent beads (Polysciences) in PBS were used ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Hek293T-cells were transfected with pNL4-3 or pCH058/2960 or pNL4-3 and pMM310 (pBlam-Vpr) via polyethylenimine (Polysciences Inc.)[67] ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Polybead Amino Microsphere 3 μm latex beads (Polysciences INC) were labeled with the fluorescent dye DyLight680 mono-N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2020Quote: ... OVA:BSA-coated 3 µm latex beads (Polysciences, Warrington, PA), or OVA-specific MHC-II peptides for 15–30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... BioMag Plus Concanavalin A coated beads (Polysciences: 86057-3) were equilibrated with Binding buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Developmental Biology 2024Quote: ... a volume of Concanavalin A beads (Polysciences, 86057-3) corresponding to 15 µl per histone mark target was prepared by removing the supernatant on a magnetic rack and washing in 1 ml binding buffer (20 mM HEPES [pH 7.5] ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 3 µL 0.1 µm Polybead microsphere suspension (Polysciences) was added to the pad ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL PEI (Polysciences, #23966-1) in 50 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescent polychromatic beads (Polysciences, 19507-5) with a 0.5 μm particle size were added to each well at a final concentration of 3.64×108 beads/mL and incubated at 20°C for 10 minutes with shaking ...
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bioRxiv - Cell Biology 2020Quote: ... from Bangs Laboratories and Latex polymer 3-micron beads (17134-15) from Polysciences. Murine IFNγ (315-05 ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 15 µl of BioMag ConcanavalinA beads (Polysciences, #86057-3) in binding buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Bioengineering 2024Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) was obtained from Polysciences Inc (Warrington ...
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bioRxiv - Cell Biology 2024Quote: ... 4.5 μm polystyrene particles (Polysciences, 17135-5) were first coated with streptavidin and next with Concanavalin A-biotin conjugate (Sigma Aldrich ...