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Citations for Polysciences :
251 - 300 of 722 citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Microbiology 2019Quote: ... followed by 1 h of fixation in 1% osmium tetroxide (Polysciences Inc.). After extensive wash with dH20 ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...
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bioRxiv - Immunology 2021Quote: ... transient transfections of 5 µg of pcDNA3.1-based vectors were performed using 25 µg of PEI MAX (Polysciences, Warrington, PA). After an additional incubation for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 8 µg of plasmid DNAs (pCMV-Thy1.1-F2A-dsGFP/muSOX/SOX/vhs/BGLF5) using 48 μl Transporter 5 (Polysciences #26008) and 1ml Opti-MemI (Gibco #31985070) ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected at a cell density of 2.5×106 cells/mL by co-transfecting plasmids (1.1 mg total DNA/L cells) using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells).
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 1% tannic acid and 1% uranyl acetate (Polysciences Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Neuroscience 2022Quote: ... vectors were transfected to 50-80% confluent HEK293T cells in 35-mm dish using PEI-MAX (25 mM, 5 μL/dish; Polysciences, Inc.). Twenty-four hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Control and 5-FU-miR-129 groups were treated with either 75 μg of PEI-MAX (control) (Polysciences, Warrington, PA, USA) or 75 μg PEI-MAX + 75 μg 5-FU-miR-129 (5-FU-miR-129) ...
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bioRxiv - Neuroscience 2022Quote: ... was injected into the gastrocnemius muscles and the tracer Fast Blue tracer (Polysciences cat.no. 17740-5 at 1.5% in 3μL PBS) was injected into the soleus muscles ...
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bioRxiv - Synthetic Biology 2023Quote: ... they were transfected by mixing roughly 600 ng of plasmid mixture per well with 1.6 μL Transporter 5 Transfection Reagent (Polysciences, Warrington, PA) in 60 μL 150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... and then mixed 1:1 with 0.2 M sodium carbonate–bicarbonate buffer (24095, Polysciences) containing 1 M sodium chloride (S671-3 ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent beads (1 μm, Polysciences) were added for internal calibration (105 mL−1) ...
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bioRxiv - Neuroscience 2020Quote: Fast Blue (Polysciences, 17740-1), CM-DiI (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... postfixed in 1% OsO4 (Polysciences) in 0.1M cacodylate buffer for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Polyethylenimine “Max” (24765-1, Polysciences) was used for transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Polyethyenimine (PEI) (Polysciences, 23966-1) was used as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... 1ml Polyethylenimine (23966-1, Polysciences), pLV delta 8.9 (16.5μg) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized using isoflurane and 1-2μl of FB (1% in saline; Polysciences Inc.) was injected subcutaneously using a Hamilton syringe connected to a 30-gauge needle to label sensory afferents that innervate the injection sites ...
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bioRxiv - Microbiology 2023Quote: ... then infiltrated in 1:1 mixture of propylene oxide:Polybed 812 epoxy resin (Polysciences, Warrington, PA) for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then mounted in a 1:1 mixture of Aqua-Poly/Mount (Polysciences, 18606) and RapiClear (SUNJin Lab ...
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bioRxiv - Cell Biology 2020Quote: ... The TEM samples were prepared by dropping 5 μl of solution onto a 400-mesh carbon film-coated copper grids (24945-50, Polysciences, Inc., PA, USA), which were glow-discharged by a plasma cleaner ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Biophysics 2023Quote: ... A volume of 20 μL of 25 mM levamisole mixed with 5 μL 200-nm-sized polystyrene beads (Polysciences Inc., Warrington, PA, USA) was dropped onto the cover glass ...
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bioRxiv - Cell Biology 2020Quote: ... or PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Microbiology 2020Quote: ... or PEI MAX40 (Polysciences 24765-1) following the manufacturers’ recommendation.
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bioRxiv - Developmental Biology 2022Quote: ... with PEI reagent (Polysciences, 23966-1). Lentiviral particles were collected 48 h and 72 h after transfection and filtered.
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bioRxiv - Genetics 2021Quote: ... or PEI MAX (Polysciences, 24765-1) according to manufacturer instructions.
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bioRxiv - Bioengineering 2022Quote: 1 µm yellow-green beads (Polysciences) were flowed through a capillary tube by pulling at 250 nL/min (rectangular capillary ...
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bioRxiv - Microbiology 2022Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer at 4ºC for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer for 30 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... PEI at 1 mg/mL (Polysciences) was used as the transfection agent ...
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bioRxiv - Bioengineering 2021Quote: ... 1 uL (or 1.5 uL for target-binding experiments) of 1 mg/mL PEI (Polysciences, 24765-2) dissolved in water was added to DNA in media and vortexed for 10 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 1 μg ml-1 DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences), pre-incubated for 15 min in Optimem medium (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...