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Citations for Polysciences :
1 - 50 of 1129 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... packaging vectors at a ratio of 4:3:1 using polyethyleneimine (PEI, Polysciences, #23966-2). Virus-containing medium was collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Bioengineering 2024Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) was obtained from Polysciences Inc (Warrington ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were fixed in 4% glutaraldehyde (Polysciences; Cat#18428-5) and 2% acrolein (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... N-(3-Aminopropyl) methacrylamide hydrochloride (APMA, >98%) was obtained from Polysciences Inc ...
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bioRxiv - Neuroscience 2024Quote: ... bEnd.3 cells were fixed in 4% cold PFA (Polysciences, Inc. PA, USA), and the immunofluorescence staining was performed following the same procedures as stated above.
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bioRxiv - Biophysics 2021Quote: ... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Physiology 2021Quote: ... for 5 min at 22 °C and mounted in Mowiol 4–88 (Polysciences) with DABCO (25 mg ml−1 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% formalin (Polysciences, 04018-1), permeabilized with 0.3% Triton X-100 in 1X PBS for 20 minutes at room temperature ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the pNL4-3-inGluc and S constructs in a 2:1 ratio using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) in order to generate viral particles ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Neuroscience 2024Quote: ... They were fixed overnight at 4°C in 4% paraformaldehyde (PFA) (Polysciences) diluted in 1X Phosphate Buffered Saline ...
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Physiology 2022Quote: ... animals were mounted on 2 % agarose dry pads with 4 % polystyrene beads (Polybeads, Polysciences) in M9 buffer ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... using 90 µL 1 mg/mL Polyethylenimine Hydrochloride (Polysciences, 24765). Growth media was harvested 24-and 48-hours post-transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... The 4 plasmids were incubated for 15 minutes with 5 μL of polyethylenimine (PEI, Polysciences #23966) before transfection ...
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bioRxiv - Immunology 2023Quote: ... polyethylenimine hydrochloride (PEI, Polysciences) was used as the transfection reagent ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Neuroscience 2023Quote: Cells for ICC were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed 4 x 5 min in PBS and mounted with Aqua-Poly/mount (Polysciences, 18606). STED images were acquired by sequential acquisition of each channel ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Neuroscience 2020Quote: ... fixed in 4% Formaldehyde (Polysciences 18814) / PEM buffer (0.1 M PIPES ...
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bioRxiv - Microbiology 2021Quote: ... were fixed with 4% formaldehyde (Polysciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2020Quote: ... fixed in 4% paraformaldehyde (Polysciences, #18814) and mounted in Prolong Diamond to preserve GFP signal were also prepared ...
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bioRxiv - Physiology 2021Quote: ... ATP [5mM] and 4% formaldehyde (Polysciences). After fixation ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
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bioRxiv - Developmental Biology 2024Quote: ... and fixed with 4% formaldehyde (Polysciences) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 200 μl of 4% PFA (Polysciences) was added to the upper chamber of the Transwells and incubated for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... with 1 mg of total DNA and 4 mg of PEI (Polysciences, 24765) per 1 liter of cells at a density of 1.5-2 x 106 cells/ml ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... at a 2:1:1 ratio using 3 μg 25 kDa linear polyethylenimine (PEI) (Polysciences Inc.) per μg DNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...