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Citations for Polysciences :
1 - 50 of 744 citations for 3 1H Pyrrol 1 yl phenyl methanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Parasites were cross-linked in 1% methanol-free formaldehyde (Polysciences Inc) at 37°C for 10 min while kept in suspension on an orbital shaker (50 rpm) ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% methanol-free formaldehyde (Polysciences) and 0.1 M potassium phosphate buffer (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were incubated in 50:50 mixture of methanol and JB4 resin (Polysciences, 00226-1, GMBH, Germany) overnight ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: For sectioning embryos in 100% methanol were mounted in JB4 plastic sectioning medium (Polysciences cat. no 00226-1). Dehydrated samples were infiltrated with 10 ml JB4 solution A plus catalyst for two days ...
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bioRxiv - Cancer Biology 2022Quote: ... and fixed with 2% methanol-free formaldehyde (Polysciences, Warrington, PA). Cells were then permeabilized with 0.1% saponin (Beckman Coulter ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formaldehyde (16%, methanol free) was purchased from Polysciences (Warrington, PA). Phosphate-buffered saline with (PBS) ...
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bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
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bioRxiv - Physiology 2024Quote: ... fixed in 3,7% methanol-free formaldehyde (Polysciences Inc., #18814-10) for 25 min ...
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bioRxiv - Bioengineering 2021Quote: ... Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photoinitiator (6 v/v %) and methacrylated rhodamine (2.5 v/v %) (Polysciences, Inc.) were added to the solution to facilitate photoinitiated crosslinking and fluorescent visualization ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... brain organoids were placed directly in 4% methanol-free formaldehyde (Polysciences) diluted in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed in 4% methanol free PFA (Polysciences Cat# 040181) for 3 hrs at room temperature (RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Microbiology 2024Quote: ... a Tat-encoding plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1:3 ratio using polyethyleneimine (PEI, Polysciences). After 8 hours ...
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Dechorionated embryos were fixed in 10% formaldehyde (methanol free, 18814 Polysciences Inc.) in PBS:Heptane (1:1 ...
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES) with formaldehyde (molecular grade methanol-free 16% formaldehyde, Polysciences Inc.) at a final percentage of 1% for 30 minutes at room temperature in a vertical rotator ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed and fixed with 4% formaldehyde (methanol-free, Polysciences). In this assay ...
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bioRxiv - Biophysics 2024Quote: ... Dechorionated embryos were fixed in 10% formaldehyde (methanol free, 18814 Polysciences Inc.) in PBS:Heptane (1:1 ...
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bioRxiv - Cancer Biology 2024Quote: ... patient tissues or GBOs were fixed with 4% methanol-free formaldehyde (Polysciences) in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed using an osmotically balanced and methanol-free 4 % formaldehyde (Polysciences) solution which has previously been shown to not cause detectable nuclear shrinkage (59) ...
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bioRxiv - Cancer Biology 2024Quote: Cells growing on coverslips were fixed in 4% methanol-free formaldehyde (PolySciences, 18814) in PBS and permeabilized with cold methanol ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Monolayer cells grown on glass coverslips were fixed in 4% methanol-free formaldehyde (Polysciences), diluted in DPBS (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was mixed 1:3 (w/w) with PEI 25 K (Polysciences, 23966-1) for 20 min at room temperature and then used to transfect HEK293S GnTi- cells at 37°C in 5% CO2 (ATCC ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3) Ad helper genes using polyethyleneimine (#23966-1, Polysciences, USA). Cells were harvested 72 hrs post transfection ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free FA (Polysciences, 040181) overnight at 4°C and stored in 100% methanol at -20°C ...
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bioRxiv - Molecular Biology 2024Quote: ... at a 2:1:1 ratio using 3 μg 25 kDa linear polyethylenimine (PEI) (Polysciences Inc.) per μg DNA ...
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bioRxiv - Immunology 2023Quote: ... 3 μl of linear PEI (1mg/ml, Cat. No. 23966-1, Polysciences) transfection reagent was used per 1 μg of plasmid DNA ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... or PEI Max (3 µg PEI per 1 µg DNA) (Polysciences 24765) (for substrate only transfection for investigating endogenous PS (Fig 5 & 6) ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free formaldehyde (FA) (Polysciences, 040181) during 2 hours at RT and then rinsed in PBSDT (PBS 1x ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...