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Citations for Polysciences :
1 - 50 of 786 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Food was prepared by resuspending 1 g of sera micron (Sera 00720) in 45 mL of 1/3 MR with 30 µL of 6 µm fluorescent bead solution (Polysciences 18862-1) added ...
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bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
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bioRxiv - Bioengineering 2021Quote: ... Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photoinitiator (6 v/v %) and methacrylated rhodamine (2.5 v/v %) (Polysciences, Inc.) were added to the solution to facilitate photoinitiated crosslinking and fluorescent visualization ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... a Tat-encoding plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1:3 ratio using polyethyleneimine (PEI, Polysciences). After 8 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... Transfection solution was prepared by combining 6 µL (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 200 µL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Biophysics 2022Quote: ... The transfection solution was prepared by combining 5 μL of 1 mg/ml polyethylenimine Max solution (Polysciences) and a plasmid mixture consisting of 200 ng ssHA-FLAG-NTS1 and 500 ng alkaline phosphatase (AP)-tagged TGF-α (AP-TGFα ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... Transfection solution was prepared by combining 20 μL (per dish, hereafter) of polyethylenimine solution (Polysciences; 1 mg/mL) and a plasmid mixture consisting of 500 ng Lg-βarr1 or Lg-βarr2 and 1μg μOR-Sm in 1 mL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Genomics 2020Quote: ... into HEK293T cells using a 1 mg/ml PEI solution (Polysciences). Lentiviral supernatants were collected at 48 hours and 72 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of a 1 μm polystyrene bead solution (Polysciences, USA), 100 μl QUEEN buffer wash ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was mixed 1:3 (w/w) with PEI 25 K (Polysciences, 23966-1) for 20 min at room temperature and then used to transfect HEK293S GnTi- cells at 37°C in 5% CO2 (ATCC ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3) Ad helper genes using polyethyleneimine (#23966-1, Polysciences, USA). Cells were harvested 72 hrs post transfection ...
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bioRxiv - Synthetic Biology 2024Quote: A 1 mg/mL solution of PEI Max (Polysciences, catalog no. 24765) was prepared for transient transfection as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... at a 2:1:1 ratio using 3 μg 25 kDa linear polyethylenimine (PEI) (Polysciences Inc.) per μg DNA ...
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bioRxiv - Neuroscience 2023Quote: ... solution (Polysciences) when necessary and were blocked and permeabilized in 3% BSA with 0.3% Triton X-100 in PBS for 1 h at room temperature ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... or PEI Max (3 µg PEI per 1 µg DNA) (Polysciences 24765) (for substrate only transfection for investigating endogenous PS (Fig 5 & 6) ...
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bioRxiv - Immunology 2023Quote: ... 3 μl of linear PEI (1mg/ml, Cat. No. 23966-1, Polysciences) transfection reagent was used per 1 μg of plasmid DNA ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with the transfection solution that was prepared by combining 8 µL (per dish, hereafter) polyethyleneimine (PEI) solution (1 mg/mL; Polysciences, Warrington, PA, USA) and a plasmid mixture consisting of 400 ng human LPAR-encoding plasmids ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Developmental Biology 2021Quote: ... testes were fixed in Bouin’s fixative solution (16045-1, Polysciences, Warrington, PA, USA) at 4°C O/N ...
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bioRxiv - Biophysics 2024Quote: ... 5000x diluted stock solution of polystyrene beads with 1 μm diameter (Polysciences, USA) in ethanol (Carl Roth ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Molecular Biology 2022Quote: A stock solution of 1 mg / ml linear Polyethylenimine (PEI; Polysciences, Inc 24765-2) was prepared in a buffer containing 25 mM Hepes ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Transfections were performed using a polyethylenimine solution (PEI, 1 mg/mL; Polysciences, Warrington, PA, USA) diluted in NaCl (150 mM ...
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bioRxiv - Bioengineering 2023Quote: ... we combined 100 ng of DNA with 1 μL of PEI MAX solution (polyethyleneimine, Polysciences) in 10 μL of DMEM and incubated at room temperature for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Cell Biology 2024Quote: ... solution (Polysciences #24310-500) for 10 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... JB4 solution A (Polysciences); GoTaq qPCR master mix ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... packaging vectors at a ratio of 4:3:1 using polyethyleneimine (PEI, Polysciences, #23966-2). Virus-containing medium was collected at 48 and 72 hours post-transfection ...
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bioRxiv - Physiology 2020Quote: ... animals were post-fixed with 1% aqueous solution of osmium tetroxide (Polysciences Europe GmbH 0972B-5) and 1.5% potassium ferrocyanide (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...