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Citations for Polysciences :
1 - 50 of 322 citations for 2 6 Amino 9H purin 9 yl ethanol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Polybead amino 6 µm-diameter microspheres (Polysciences) were prepared to covalently couple rCD6 or rICAM1 ...
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bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µl of 6-µm-diameter polystyrene MB suspension (containing 6.3 × 106 beads, Polysciences), and 1 µl of PBS containing 30% Healon was injected into the anterior chamber using a 32-gauge needle (Hamilton Company ...
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bioRxiv - Cell Biology 2023Quote: ... cells were initially seeded at 2× 105 per 2 ml in 6-well plates and transfected using PEI MAX 40K (Polysciences Inc. # 24765-100) (1:3 DNA to PEI ratio) ...
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bioRxiv - Physiology 2024Quote: ... Slides were counterstained with eosin (0.3% in ethanol; Polysciences cat#02740).
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bioRxiv - Cell Biology 2020Quote: Polybead Amino Microsphere 3 μm latex beads (Polysciences INC) were labeled with the fluorescent dye DyLight680 mono-N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 100 mm diameter dishes to 90% confluency and were subsequently transfected with 6 μg of pCG1_SARS-2-S plasmid using polyethylenimine (PEI) Max (MW = 40,000KDa, Polysciences, Germany) as transfection reagent ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and the slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Physiology 2020Quote: ... were transiently co-transfected with either A1R-Nluc or A3R-Nluc and β-arrestin2-YFP (total 2 μg, at a 1:6 ratio) using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Microbiology 2020Quote: 293T cells were grown to ~70% confluence and transfected with a mixture of 2 μg of DNA encoding the gRNA and 6 μL of Polyethylenimine (PEI, Polysciences catalogue #24765 ...
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bioRxiv - Cell Biology 2021Quote: ... dehydrated in ethanol and embedded in Polybed 812 epoxy resin (Polysciences, Inc., Warrington, PA). 80nm ultrathin sections were cut ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were dehydrated with an ethanol gradient before being embedded in JB4 resin (Polysciences). Samples were sectioned at 5 µm with a microtome Rotatif ...
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bioRxiv - Microbiology 2021Quote: ... one half of the ovaries of each of 6 crickets was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, USA) in 100 mM phosphate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Developmental Biology 2020Quote: ... dehydrated in ethanol and embedded in epoxy resin poly/Bed 812 (Polysciences Inc., Warrington, Pennsylvania). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... gradient dehydration in ethanol and embedment in Polybed 812 epoxy resin (Polysciences, Inc., Warrington, PA). Grid-mounted 80 nm ultrathin sections were stained with 4% uranyl acetate and examined by transmission electron microscopy using a JEOL JEM-1230 transmission electron microscope operating at 80 kV (JEOL USA ...
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bioRxiv - Bioengineering 2024Quote: ... Alcian blue (Fisher, cat#92-31-9) and nuclear fast red (Polysciences, cat# 09773) sections were stained with alcian blue (pH = 0.2 ...
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bioRxiv - Cell Biology 2020Quote: ... dehydrated in graded ethanol series and embedded with Epon-filled BEEM capsules (EMS; Polysciences, Inc., 00224) placed on top of the Mattek dish ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were dehydrated with increasing amounts of ethanol and embedded in epon epoxy resin (Polysciences). Ultrathin sections of 60 nm were contrasted with uranyl acetate and lead citrate using an AC20 automatic contrasting system (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... dehydrated in graded ethanol series and embedded with Epon-filled BEEM capsules (EMS; Polysciences, Inc., 00224) and placed on top of the Mattek dish ...
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bioRxiv - Neuroscience 2024Quote: ... and frozen in a mixture of dry ice and ethanol in embedding molds (Polysciences 18646A-1) and left at -80 °C for up to one year ...
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bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were dehydrated with serial ethanol treatment and subsequently embedded in Durcupan resin/propylene oxide (Polysciences). Approximately 100 nm thin sections on copper grids were observed at 80 kV with a JEOL JM2100 transmission electron microscope.
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... They were dehydrated in a graded ethanol series and propylene oxide and embedded in Poly/BedR 812 (Polysciences Inc.). Ultrathin sections were contrasted with uranyl acetate and lead citrate ...
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bioRxiv - Developmental Biology 2020Quote: ... dehydrated in a graded ethanol series and propylene oxide and embedded in Poly/BedR 812 (Polysciences, Inc., Eppelheim, Germany). Ultrathin sections were contrasted with uranyl acetate and lead citrate ...
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bioRxiv - Neuroscience 2024Quote: ... Dehydration using a graded ethanol series followed by propylene oxide incubation and resin infiltration with Polybed812 epoxy resin (Polysciences). Blocks were polymerized for 48 hours at 60°C.
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... and LPS (100 μg/ml) coupled to 3-μm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were dehydrated in graded ethanol solutions and infiltrated with graded epoxy resin (Poly/Bed 812, Polysciences, Warrington, PA) / propylene oxide for 14 days before polymerization at 60 °C.
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bioRxiv - Cell Biology 2021Quote: ... 200 μL of 6% paraformaldehyde (Polysciences 18814-10) in PBS was added to each well and incubated for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Cell Biology 2020Quote: ... The tissue slides were then dehydrated in an increasing gradient of ethanol (95%, 100%, 100%) and re-paraffinized with xylene and mounted in Poly-Mount (Polysciences, Inc.). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then dehydrated with increasing concentrations of ethanol (50-100%),infiltrated with propylene oxide for 10 min and embedded with a Poly/Bed 812 kit (Polysciences, USA) and polymerized for 18 hours at 60°C ...
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Cell Biology 2022Quote: T24 cells were fixed in 2% glutaraldehyde and 2% paraformaldehyde at 4°C overnight and then postfixed in 2% osmium tetraoxide for 1.5 h at 4°C followed by dehydration with ethanol and embedding in Spurr resin (Polysciences Inc., Warrington, PA). Ultrathin sections of the samples were prepared using Ultramicrotome (Leica EM UC7 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were treated with glutaraldehyde and osmium tetroxide and then dehydrated through a graded series of ethanol before they were embedded in Spurr’s resin (Polysciences Inc., Warrington, PA). Rapid freezing and the freeze-substitution method were performed as previously described (36) ...
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bioRxiv - Microbiology 2024Quote: ... 6.5 µg of psPAX2 and 9 µg of either GeCKO v2 human sublibrary A or B (Table S3) using PEI MAX transfection reagent (Polysciences). After 48 hours ...
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bioRxiv - Biophysics 2020Quote: ... we dispensed 6 μm diameter polystyrene beads (Polybead microspheres, Polysciences) diluted in HBSS into recording μ-slide channels and let them settle to the bottom of the μ-slide ...
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bioRxiv - Biophysics 2023Quote: ... Latex microspheres of 6 µm diameter (Polysciences Inc., PA, USA) were used to determine the zero-pressure level ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Cell Biology 2022Quote: ... AAV-pro 293T cells were transfected with gRNA vectors along with AAV packaging vectors AAV2/9 and pAdDeltaF6 using the PEI MAX transfection reagent (Polysciences, 24765). 72 hours after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 2 ml 2% PFA (Polysciences, Inc., 18814). After 30 min incubation at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10,000 photons were recorded after measurement of the internal response function with Polybead amino 0.10 micron microspheres (Polysciences, Warrington, PA, USA). Data was analyzed with version 3.0.0.80 of the Quantaurus-Tau software.