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Citations for Polysciences :
1 - 50 of 890 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... packaging vectors at a ratio of 4:3:1 using polyethyleneimine (PEI, Polysciences, #23966-2). Virus-containing medium was collected at 48 and 72 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... nadic methyl anhydride (NMA, Polysciences Inc., cat# 00886–500), and 2,4,6-Tris-(dimethylaminomethyl)phenol (DMP- 30 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Neuroscience 2024Quote: ... bEnd.3 cells were fixed in 4% cold PFA (Polysciences, Inc. PA, USA), and the immunofluorescence staining was performed following the same procedures as stated above.
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bioRxiv - Immunology 2023Quote: ... One coverslip was activated with 3-aminopropyltriethoxysilane using 0.1 M NaOH (Macron Fine Chemicals) and 0.05% glutaraldehyde (Polysciences Inc.) and the other coated with dichloromethylsilane (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... a Tat-encoding plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1:3 ratio using polyethyleneimine (PEI, Polysciences). After 8 hours ...
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% formalin (Polysciences, 04018-1), permeabilized with 0.3% Triton X-100 in 1X PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was mixed 1:3 (w/w) with PEI 25 K (Polysciences, 23966-1) for 20 min at room temperature and then used to transfect HEK293S GnTi- cells at 37°C in 5% CO2 (ATCC ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3) Ad helper genes using polyethyleneimine (#23966-1, Polysciences, USA). Cells were harvested 72 hrs post transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... at a 2:1:1 ratio using 3 μg 25 kDa linear polyethylenimine (PEI) (Polysciences Inc.) per μg DNA ...
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bioRxiv - Immunology 2023Quote: ... 3 μl of linear PEI (1mg/ml, Cat. No. 23966-1, Polysciences) transfection reagent was used per 1 μg of plasmid DNA ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... or PEI Max (3 µg PEI per 1 µg DNA) (Polysciences 24765) (for substrate only transfection for investigating endogenous PS (Fig 5 & 6) ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Neuroscience 2024Quote: ... into one 15-cm plate of confluent HEK-293T cells using PEI Max (Polysciences Inc., catalog # 24765-1). At one day post-transfection we changed the medium to low serum (1% FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Biochemistry 2020Quote: ... Contrasting for TEM was done by embedding the grids in 1.8% methyl cellulose and 0.4% uranyl acetate (Polysciences, Inc. ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Neuroscience 2023Quote: Cells for ICC were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Microbiology 2023Quote: ... one day before plasmid transfection with polyethylenimine (PEI, Polysciences, Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cancer Biology 2024Quote: Poly(methyl methacrylate-co-methacrylic acid) (PMMA-MA; 10% methacrylic acid, MW ∼100,000 Da, 17913-500) was from Polysciences (USA), organic solvents were obtained from Carl Roth (Austria) ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... with 1 mg of total DNA and 4 mg of PEI (Polysciences, 24765) per 1 liter of cells at a density of 1.5-2 x 106 cells/ml ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).