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Citations for Polysciences :
401 - 450 of 870 citations for Monoethyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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bioRxiv - Synthetic Biology 2019Quote: ... A master stock of PEI was made by dissolving linear PEI (Polysciences Cat. No. 23966-2) in deionized water at a concentration of 0.323g/L ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pre-permeabilised in 0.5% Triton X-100 in cytoskeleton buffer for 15 s and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were transfected in 100 mm dishes using 8 µg plasmid DNA and 24 µl PEI (Polysciences, Inc). DNA was mixed with PEI in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... elegans larvae were mounted on a 10% agarose pad in a solution of 100 nm polystyrene microbeads (Polysciences Inc.) to stop worm movement ...
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bioRxiv - Neuroscience 2023Quote: AAV8 viruses were produced in HEK293FT cells grown on 5 plates (linear PEI, Polysciences Europe Cat. No. 23966-100) or 10 plates (branched PEI ...
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bioRxiv - Biophysics 2023Quote: Cells were pre-permeabilized in 0.5% Triton X-100 in cytoskeleton buffer for 15 s for tubulin and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... For this study 1:100 dilution of bead solution in double distilled water (Flurospheres amine-modified, 0.2 μm, yellow-green fluorescent, Microspheres, Polysciences) was used ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid co-transfections were performed with 1mg/ml PEI MAX (24765, Polysciences) with DNA:PEI at a ratio of 1:3 as follows ...
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bioRxiv - Neuroscience 2020Quote: ... Polyethylenimine 40 µg/ml (PEI, Linear, MW 25000, Transfection Grade, Polysciences Inc.) was used as a transfection reagent and incubated overnight at 37°C ...
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bioRxiv - Genetics 2021Quote: ... expression plasmids into HEK293T cells using a 1mg/mL PEI solution (Polysciences). We collected lentiviral supernatants at 48 hours and 72 hours after transfection and concentrated lentiviruses by ultracentrifugation for 120 minutes at 19,500 rpm using a Beckman SW28 ultracentrifuge rotor at 4°C.
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bioRxiv - Molecular Biology 2022Quote: ... or 2.5 μl of PEI-max (1.0 mg/ml) (Polysciences, Warrington, PA). Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Capture of VirB was performed on a 30 mL phosphocellulose (Polysciences, Inc.) column ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Developmental Biology 2019Quote: Fluorescent red beads of 1 uM diameter (1:500, 18660-5, Polysciences) were injected into the KV of embryos at the 6-somite stage ...
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bioRxiv - Microbiology 2019Quote: ... followed by 1 h of fixation in 1% osmium tetroxide (Polysciences Inc.). After extensive wash with dH20 ...
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bioRxiv - Genetics 2021Quote: ... Masson’s Trichrome staining was performed on frozen sections using the Masson’s Trichrome stain kit (Cat# 25088-100; Polysciences; Warrington, PA) and the following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Pandoravirus massiliensis virions were then placed for 1h in a mix of LR-White resin 100% (Ref. 17411, MUNC-500; Polysciences) and ethanol 96% in a 2:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... animals were soaked in ScaleA2 (4M urea, 20% glycerol, 0.1% Triton X-100, 2.5% DABCO)38 and mounted in Aqua-Polymount (Polysciences Inc. 18606). Some in situ hybridizations were carried out in a CEM InSituPro Hybridization robot up to the development stage ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293T cells were seeded into a 100 mm dish and transfected with 6 μg of FLAG-USP-46 using Polyethylenimine (PEI, PolySciences) for 16-18 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µg of DNA diluted in 100 µL of NaCl 150 mM was mixed with 6 µL of PEI Max 40000 (Polysciences) at 2 mg/mL diluted in 100 µL of NaCl 150 mM ...
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bioRxiv - Genetics 2023Quote: ... coverslips were washed twice with PBS supplemented with 0.1 % Triton-X-100 and once with PBS and mounted with aqua-poly/mount (Polysciences Inc). Cells were imaged using a Zeiss LSM700 microscope equipped with a Plan-Apochromat 40x/1.3 Oli DIC M27 immersion lens (Carl Zeiss Microimaging Inc.) ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA with the amount of 10 μg was mixed with 30 μg of linear polyethyleneimine (PEI) (23966-100; Polysciences) in 1 ml of serum-free DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were co-transfected with LentiCRISPR v2 plasmids along with packaging plasmids psPAX2 and pMD2.G using PEI transfection reagent (#23966-100, Polysciences). Lentiviruses were collected 48 hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...
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bioRxiv - Biophysics 2021Quote: ... and 4uL of 1mg/mL PEI (Polyethylenimine HCl MAX Linear 40K from Polysciences Catalog No ...
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bioRxiv - Immunology 2023Quote: ... and 2.2 μg/ml polyethylenimine (linear 25 kDa PEI, Polysciences, Inc, Warrington, PA) to the suspension cultures ...
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Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... were transfected with 3μg of plasmid DNA/ml using Polyethylenimine HCl MAX (Polysciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and 500 µL of the melted agarose was supplemented with 15 µL of 2-µm polystyrene beads (Polysciences). Twenty µL each of the FP and agarose/beads solutions were mixed thoroughly ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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bioRxiv - Microbiology 2021Quote: ... spores were harvested in KK2 buffer and fixed with 2% paraformaldehyde with 2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Live animals were immobilized with 0.01% tetramisole and 0.1% tricaine in M9 buffer and mounted on 2% agarose pads or with 0.05 μm Polybead microspheres (Polysciences) diluted 1:2 in water and mounted on 5% agarose pads ...
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bioRxiv - Developmental Biology 2019Quote: ... and triple transfected into 10 15-cm plates of HEK293T cells using PEI transfection reagent (Polysciences, 23966- 2). 60 h after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by 2 minutes incubation of the slices in 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences). rinsed 5 × 2 min in chilled buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 1% tannic acid and 1% uranyl acetate (Polysciences Inc. ...