Labshake search
Citations for Polysciences :
401 - 450 of 568 citations for 7 Aminocarbonyl amino 4 hydroxy 3 4 4 sulfophenyl azo phenyl azo naphthalene 2 sulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... and psPAX.2 (3.75 μg) with PEI MAX 40K (Polysciences Inc., # 24765-100) (1:3 DNA to PEI ratio) ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected with BirAGFP-KIF13B Tail 2 or Tail 3 constructs (designated C2 and C3, respectively, in (Serra-Marques et al., 2020)) using Polyethylenimine (PEI; Mw 2500; Polysciences) at a 3:1 PEI:DNA ratio (w/w) ...
-
bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
-
bioRxiv - Biochemistry 2019Quote: ... followed by ∼3 microliters of the final assay solution containing 5 ng mL-1 500 nm diameter beads (PolySciences Inc,), coated nonspecifically with neutravidin18(ThermoFisher Inc.).
-
bioRxiv - Cell Biology 2019Quote: ... samples were washed 3 times for 30 mins with PBS then incubated through a cryoprotective series of sucrose solutions of increasing concentration from 10-50% for ≥3 hours each before embedding into OCT compound (Tissue Tek) in disposable cryomolds (Polysciences) and frozen in an isopentane bath cooled by liquid nitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
-
bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
-
bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
-
bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
-
bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
-
bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
-
bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
-
bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
-
bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
-
bioRxiv - Neuroscience 2019Quote: ... and 1 µm (2 µL at 1.5×107 beads/µL; Polysciences, Cat#: 07310-15) polystyrene microbeads through a 50-100 µm cannula in the cornea formed by a beveled glass micropipette connected by polyethylene tubing to a Hamilton syringe (Hamilton Company Reno ...
-
bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then washed and fixed with 2% ultra-pure formaldehyde (Polysciences, Warrington, PA) for 20 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: A stock solution of 1 mg / ml linear Polyethylenimine (PEI; Polysciences, Inc 24765-2) was prepared in a buffer containing 25 mM Hepes ...
-
bioRxiv - Neuroscience 2022Quote: ... 1.5 μl retrograde tracer Fast Blue (2% w/v in 0.9% saline; Polysciences, Germany) was injected into the operated stifle joints 7 days prior to mouse sacrifice to label knee innervating neurons.
-
bioRxiv - Biophysics 2021Quote: ... The water surrounding the spheres was seeded with 2 µm polystyrene spheres (Polysciences 19814). PIV analysis was performed using PIVlab [53] ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with polyethylenimine (PEI; linear, MW 25,000; Polysciences, cat. No. 23966-2) at a 2:1 ratio (PEI:DNA ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were picked up onto 1×2 mm formvar coated copper slot grids (Polysciences), and further stained with uranyl acetate followed by lead citrate ...
-
bioRxiv - Immunology 2021Quote: ... Small-scale vector preparations were generated by polyethylenimine or PEI (Polysciences, Cat #24765-2) triple transfection of AC1 or AC3 ITR-flanked transgene ...
-
bioRxiv - Biochemistry 2022Quote: ... An aqueous 10µg/ml stock of linear 25kDa PEI (Polysciences, catalogue number 23966-2) was used for the transfection ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 19.5 μg of psPAX2 were transfected using polyethylenimine (1μg/μl, Polysciences # 23966-2) into 293T cells plated in 6-well plates ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 µl of 6-µm-diameter polystyrene MB suspension (containing 6.3 × 106 beads, Polysciences), and 1 µl of PBS containing 30% Healon was injected into the anterior chamber using a 32-gauge needle (Hamilton Company ...
-
bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
-
bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
-
bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
-
bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
-
bioRxiv - Neuroscience 2019Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg): PEI (μL) (Polysciences, USA) at 1mg/mL in molecular grade water ...
-
bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
-
bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
-
bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
-
bioRxiv - Immunology 2023Quote: ... 0.5 mM spermidine in 1X protease inhibitor buffer) and incubated with activated concanavalin A (ConA) coated magnetic beads (Polysciences - 86057-3) in PCR strip tubes at room temperature for 10 minutes ...
-
bioRxiv - Cell Biology 2020Quote: Third-generation VSV.G pseudotyped HIV-1 lentiviruses were produced by polyethylenimine (PEI, Polysciences #23966-2)-transfection of HEK293T cells and packaged with VSVG-coats using established methods ...
-
bioRxiv - Microbiology 2021Quote: ... highly synchronized infected RBCs were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... Streptavidin-conjugated Yellow-Green latex beads (2 μm diameter, 5 x 108 beads/preparation, Polysciences) were washed with 1% BSA in PBS and incubated with Biotin-SP (long spacer)-conjugated Fab fragments of goat-anti-mouse IgG (H+L ...