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Citations for Polysciences :
401 - 450 of 1076 citations for 6H Dipyrido 3 2 b 2' 3' e 1 4 diazepin 6 one 11 ethyl 5 11 dihydro 8 2 hydroxyethyl 5 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Plasmids (500 ng DNA per ml culture) and polyethylenimine (MW 25,000; Polysciences; 3 µg per ml culture) were mixed with OptiMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Neuroscience 2020Quote: ... sections were subsequently washed with PBS 1X (3 × 10 min) and mounted in Aqua/polymount (Polysciences 18606).
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 0.4 mg each of TCR and CD3 DNA were mixed with 3 mg of PEI MAX (Polysciences) and added to 0.8 L of HEK293F cells grown in suspension in FreeStyle 293 media (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: Slices were washed 3 times for 10 minutes in PBS and mounted with Aqua-Poly/Mount (Polysciences). Images were taken using a Zeiss LSM780 confocal microscope and images were processed using ImageJ software ...
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bioRxiv - Neuroscience 2021Quote: ... Cell counts were calculated by using 15 μm polystyrene microbeads (#18328-5, Polysciences).
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bioRxiv - Cell Biology 2021Quote: ... or into a bead slurry containing 20 µm polystyrene beads (Polysciences - #18329-5) in M9 + 0.5% (w/v ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfected using the Transporter 5 transfection reagent (Cat#: 26008, Polysciences) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... Finally sections were subsequently washed with PBS (3 x 10 min) and mounted in Aqua/polymount (Polysciences, 18606).
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... pNL4-3 and pNL4-3dNef were transfected into HEK293T cells using polyethylenimine (PEI) transfection reagents (Polysciences, PA, USA). At 48 h post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Developmental Biology 2022Quote: ... Exogenous plasmid DNA was transfected using Transporter™ 5 transfection reagent (Polysciences, PA, USA). Co-transfection of siRNA with plasmid DNA was performed using jetPRIME® transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293T cells were performed using the Transporter 5 Transfection Reagent (Polyethylenimine; Polysciences) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... and covers were mounted with Aqua-Poly/Mount solution (cat#18606-5 from Polysciences Europe) on glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% formalin (Polysciences, 04018-1), permeabilized with 0.3% Triton X-100 in 1X PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected with BirAGFP-KIF13B Tail 2 or Tail 3 constructs (designated C2 and C3, respectively, in (Serra-Marques et al., 2020)) using Polyethylenimine (PEI; Mw 2500; Polysciences) at a 3:1 PEI:DNA ratio (w/w) ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed 3 times for 30 mins with PBS then incubated through a cryoprotective series of sucrose solutions of increasing concentration from 10-50% for ≥3 hours each before embedding into OCT compound (Tissue Tek) in disposable cryomolds (Polysciences) and frozen in an isopentane bath cooled by liquid nitrogen ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μg each of DNA constructs respectively were used with polyethylenimine (PEI; Polysciences, Eppelheim, Germany) as previously described (8 ...
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bioRxiv - Immunology 2022Quote: ... DAPI for 5 minutes and then cover slipping the tissue sections with Aqua-Poly mount (Polysciences). CD25+ cells were detected after the TBS wash step by blocking with 3% H2O2 for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl (10^10 microspheres/mL) of Fluoresbrite® Multifluorescent Microspheres 0.2 µm (PolySciences 24050-5) were spread onto a bilayer plate using sterile glass beads and dried for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS twice for 5 min and mounted with Aqua PolyMount medium (PolySciences). Finally ...
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bioRxiv - Cell Biology 2023Quote: Evaluation of phagocytic uptake was performed using Fluoresbrite® BB Carboxylate Microspheres 1.75µm (Polysciences, 17686-5). Opsonization of the microspheres was performed by incubation in 50% FBS in PBS for 30 min at 37°C ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Adeno-associated viruses (AAVs) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed for 1 h at 4°C in 4% paraformaldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Immunology 2023Quote: ... 0.5 mM spermidine in 1X protease inhibitor buffer) and incubated with activated concanavalin A (ConA) coated magnetic beads (Polysciences - 86057-3) in PCR strip tubes at room temperature for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and stained with Picrosirius Solution B (Polysciences, 24901B) for one hour at room temp ...
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bioRxiv - Neuroscience 2020Quote: ... We mounted sections on coated slides and dipped slides in Ilford K.5 nuclear tract emulsion (Polysciences, 1:1 dilution in double-distilled water ...
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were freeze substituted in 0.1– 5% uranyl acetate in acetone and embedded in Lowicryl HM20 (Polysciences) with automated temperature control (Leica Microsystems EM-AFS2) ...
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bioRxiv - Cell Biology 2021Quote: ... The entire intestine was dissected out and fixed in 1× PBS plus 8% EM-grade paraformaldehyde (Polysciences) for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... were purchased from Sigma Aldrich while PSS of Mw = 1 000 (PSS_5) and Mw = 8 890 (Mw/Mn = 1.09) (PSS_43) were bought from Polysciences and Scientific Polymer Products Inc. ...
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bioRxiv - Immunology 2022Quote: ... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
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bioRxiv - Microbiology 2019Quote: ... were co-transfected with 3 µg Gibson Assembly product DNA plus 330 ng pHEF-VSV-G using polyethylenimine (Polysciences, Inc., Warrington, PA) at a ratio of 1 µg total DNA to 4 µg polyethylenimine in 800 µl of 150 mM NaCl [72] ...
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bioRxiv - Physiology 2024Quote: ... The balloon was inflated after the first diagonal branch of the LAD in each animal and 3 ml polystyrene microspheres (Polybead, 90 μm, Polysciences, Warrington, PA) were slowly injected via the lumen of the angioplasty catheter ...
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bioRxiv - Biochemistry 2020Quote: ... Contrasting for TEM was done by embedding the grids in 1.8% methyl cellulose and 0.4% uranyl acetate (Polysciences, Inc. ...