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Citations for Polysciences :
351 - 388 of 388 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Bioengineering 2021Quote: ... and then transfected with 0.75 μg of an equimolar mixture of the three third-generation packaging plasmids (pMD2.G, psPAX2, pMDLg/pRRE) and 0.75 μg of LSR vectors using 10 μl of polyethylenimine (PEI, Polysciences #23966) and 200 μl of cold serum free DMEM ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA with the amount of 10 μg was mixed with 30 μg of linear polyethyleneimine (PEI) (23966-100; Polysciences) in 1 ml of serum-free DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... were used The slides were again washed and stained with DAPI (Thermo-Fisher, 300 nM) for 10 min prior to mounting with Aqua PolyMount medium (PolySciences).
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bioRxiv - Cell Biology 2022Quote: ... embryos were dissected in 8-10 μL of Shelton’s Growth Medium (Edgar and Goldstein, 2012) and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 48 hours prior to fixation with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: Poly(methyl methacrylate-co-methacrylic acid) (PMMA-MA; 10% methacrylic acid, MW ∼100,000 Da, 17913-500) was from Polysciences (USA), organic solvents were obtained from Carl Roth (Austria) ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mm dishes of HEK293T cells (∼80% confluent) were transfected with 10 μg of plasmid using 60 μg of linear Polyethylenimine 25 kDa (23966, Polysciences Inc) mixed in 2 mL Opti-MEM (31985070 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then dehydrated with increasing concentrations of ethanol (50-100%),infiltrated with propylene oxide for 10 min and embedded with a Poly/Bed 812 kit (Polysciences, USA) and polymerized for 18 hours at 60°C ...
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bioRxiv - Cell Biology 2024Quote: HEK 293T cells on 15 x cm2 dishes were transfected with 3.33 mg of each construct for a final 10 μg of total DNA using 25 μL polyethylenimine (PEI; Polysciences, Warrington, PA). Cells were lysed mechanically in a hypotonic solution (20 mM Tris-HCl (pH 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... was co-transfected with a pcDNA5/FRT/TO plasmid containing the gene of interest in a 1:10 ratio using polyethylenimine “MAX” (Polysciences, 24765). After medium exchange 6 h post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... negative staining was performed by depositing for 10 minutes with the gird membrane side down on a drop of 1% (w/v) uranyl acetate (Polysciences, USA). The remaining solution on the grid was removed using filter paper (Whatman ...
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bioRxiv - Neuroscience 2024Quote: ... A similar composition of microglia were plated in monoculture for phagocytosis of synaptosomes and fluorescent beads (Polysciences; Cat. No. 15702-10). Synaptosomes were prepared using iNeurons expressing the synaptophysin-Gamillus construct and SynPer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Worms were mounted without anesthetic on 10% agarose pads in a 1:1 solution of M9 buffer and 0.1 μM polystyrene beads (Polysciences, Inc., Warrington, PA) essentially as described (Rosu and Cohen-Fix 2020) ...
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bioRxiv - Biochemistry 2023Quote: HEK293T cells cultured in DMEM with 10% Fetal Calf Serum were transfected using linear polyethylenimine (MW ∼25,000, Cat#23966 Polysciences Inc., Warrington, PA), 40 µg/ 10 cm dish and plasmid DNA ...
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bioRxiv - Bioengineering 2022Quote: PEG-αMA hydrogels were fabricated at 17.5 wt% with a 0.375 molar ratio of 70:30 DTT:MMP2-degradable as described above and swollen overnight in PBS with 2.2 mM LAP and 10 µM methacryloxyethyl thiocarbamoyl rhodamine B (Polysciences Inc., Warrington, PA). Swollen hydrogels were exposed to 10 mW cm−2 365-nm light for five minutes through a chrome-on-quartz photomask with spatially patterned opaque and transparent regions ...
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bioRxiv - Immunology 2023Quote: 50,000 of thymocytes from Satb1+/Venus mice were resuspended in 0.1mL RPMI-1640 medium (10% FBS) and were mounted on a poly-L-lysine-coated glass slide (Polysciences, Inc., REF: 26414) and incubated at 37 °C for 2 hours ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The decalcified tibiae were immersed in a 1:1 mixture of 5% potassium dichromate and 2% osmium tetroxide (23310-10, Polysciences, Inc., USA) for 48 h and stained ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of pHEF-VSV-G into 70% confluent monolayers of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were transferred onto 10% agarose pads in M9 buffer and immobilized with Polybead polystyrene 0.1 mm microspheres (Polysciences Inc., Warrington, PA, USA).
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bioRxiv - Neuroscience 2021Quote: ... adult hermaphrodite worms were immobilized on 10% agarose pads under a coverslip using the friction produced by 0.10 µm diameter polystyrene beads (Polysciences, Inc. Catalog number 00876) and imaged using an inverted microscope (Axiovert S100 ...
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bioRxiv - Microbiology 2023Quote: HEK293T cells (2×106 cells on a 10 cm dish) were transfected with 12 μg of plasmid DNA and 36 μg of polyethylenimine hydrochloride/PEI MAX (Polysciences, Inc., PA, USA). The cells were then cultured for 20 h ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells (2 × 106 cells on a 10 cm dish) were transfected with 12 μg of plasmid DNA and 36 μg of polyethylenimine hydrochloride (PEI) MAX (Polysciences, Inc., PA, USA) for 20 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were infiltrated with propylene oxide for 10 min and embedded using a Poly/Bed 812 kit (Polysciences, Warrington, PA, USA; Cat:08792). The specimens were sectioned at 200 nm using an ultramicrotome (EM-UCT ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were then washed for 10 minutes in PBS (3 times) and stained with a 1:1 mixture of 2% aqueous osmium tetroxide (cat# 23310-10, Polysciences, Inc., Warrington, PA, USA) and 5% potassium dichromate for 48 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were either kept whole or dissected further into ovarioles and fixed for 10 mins using 16% methanol-free formaldehyde (Polysciences, Inc., Warrington, PA, USA) diluted to a final concentration of 4% in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... adult worms were mounted on 8-10% agarose pads on glass slides and immobilized using 0.1 μm polystyrene beads (Polysciences, Warrington, PA, 2.5% by volume) diluted 2-fold in PBS (pH 7.4 ...