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Citations for Polysciences :
351 - 400 of 674 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 minutes and mounted using AquaPolymount (Polysciences, Warrington, USA). Images were obtained on a Confocal and STED FACILITY line microscope (Abberior Instruments ...
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bioRxiv - Biochemistry 2020Quote: Plasmids (500 ng DNA per ml culture) and polyethylenimine (MW 25,000; Polysciences; 3 µg per ml culture) were mixed with OptiMEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Neuroscience 2020Quote: ... sections were subsequently washed with PBS 1X (3 × 10 min) and mounted in Aqua/polymount (Polysciences 18606).
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 0.4 mg each of TCR and CD3 DNA were mixed with 3 mg of PEI MAX (Polysciences) and added to 0.8 L of HEK293F cells grown in suspension in FreeStyle 293 media (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: Slices were washed 3 times for 10 minutes in PBS and mounted with Aqua-Poly/Mount (Polysciences). Images were taken using a Zeiss LSM780 confocal microscope and images were processed using ImageJ software ...
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bioRxiv - Neuroscience 2021Quote: ... Cell counts were calculated by using 15 μm polystyrene microbeads (#18328-5, Polysciences).
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bioRxiv - Neuroscience 2020Quote: ... we used FastBlue (5% in distilled water, 17740-1, Polysciences, Inc, Warrington, PA), fluorescent latex microspheres (red retrobeads IX or green retrobeads IX ...
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bioRxiv - Cell Biology 2021Quote: ... or into a bead slurry containing 20 µm polystyrene beads (Polysciences - #18329-5) in M9 + 0.5% (w/v ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfected using the Transporter 5 transfection reagent (Cat#: 26008, Polysciences) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: Flies were dissected in ice-cold PBS and fixed with 4% PFA in PBS (Polysciences) for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were rinsed before being cover-slipped with mounting media (Mowiol 4-88, Polysciences, 17951) containing an anti-fade reagent (p-Phenylenediamine ...
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bioRxiv - Cell Biology 2020Quote: EVs were incubated overnight (4°C) with polystyrene microspheres 4.5 µm (Polysciences INC, Warrington, PA). The microspheres were blocked with 2% BSA (v/w ...
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bioRxiv - Biophysics 2022Quote: ... for 1 hour and finally mounted on glass slides with Mowiol 4-88 (Polysciences, Inc.) and kept at 4°C overnight.
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bioRxiv - Microbiology 2020Quote: ... and both the apical and basal parts were fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.), and blocked with 10% goat serum (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips with the hippocampal cultures were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Neuronal images were acquired using an Olympus IX81 inverted epifluorescence microscope at 63× optical magnification with a CCD camera (Princeton MicroMax ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed in 4% paraformaldehyde/0.01% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... and both the apical and basal parts were fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.) in 1x PBS for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were fixed in cold 4% paraformaldehyde (PFA) (Polysciences, Inc. Warrington, PA, USA) at room temperature for 20 min and washed with staining medium (PBS containing 0.1% NaN3 and 2% FCS) ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of 1 mg/mL of polyethylenimine HCl MAX Linear MW 40k (PEI, Polysciences) in 25 mM HEPES pH 7.5 was applied and incubated on glow discharged grids for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Neuroscience 2020Quote: ... Finally sections were subsequently washed with PBS (3 x 10 min) and mounted in Aqua/polymount (Polysciences, 18606).
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bioRxiv - Cell Biology 2022Quote: ... 2-3μl of 1.25% (w/v) polystyrene microspheres (Polybead Microspheres 0.05μm, Polysciences) and 0.2mM levamisole (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently transferred to 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... were produced as previously described using a PEI (polyethylenimine, Polysciences, 24765-2) transfection protocol followed by iodixanol gradient purification ...
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bioRxiv - Cell Biology 2022Quote: ... and 48 μL of Polyethylenimine ‘Max’ MW 40,000 Da (Polysciences; 24765-2) per plate ...
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bioRxiv - Neuroscience 2023Quote: ... with 2 μl of 100 nm polystyrene beads (Cat# 00876-15, Polysciences) and 2 μl of levamisole (from 500 μM stock ...
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bioRxiv - Pathology 2024Quote: ... Vector preparations were generated by polyethylenimine or PEI (Polysciences, Cat #24765-2) triple transfection of pACM-Delta ITR-flanked transgene ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS or HeLa cells were transfected using polyethylenimine (PEI) (23966-2 Polysciences) at a 1:3 plasmid to PEI ratio ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg DNA/ml transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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bioRxiv - Developmental Biology 2022Quote: ... Exogenous plasmid DNA was transfected using Transporter™ 5 transfection reagent (Polysciences, PA, USA). Co-transfection of siRNA with plasmid DNA was performed using jetPRIME® transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293T cells were performed using the Transporter 5 Transfection Reagent (Polyethylenimine; Polysciences) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... paraffin-embedded livers were sectioned at 5 µm thickness and stained with hematoxylin (Polysciences) and eosin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free FA (Polysciences, 040181) overnight at 4°C and stored in 100% methanol at -20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and chemically fixed by re-suspending in a buffer containing 4% formaldehyde (Polysciences, Inc., Warrington, USA), 80 mM HEPES-K ...
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bioRxiv - Biophysics 2021Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Plant Biology 2021Quote: Material collected for microscopy was fixed in 4% v/v acrolein (Polysciences, New Orleans, Louisiana, USA) in 1X PIPES buffer (50 mmol/L PIPES ...
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bioRxiv - Biophysics 2020Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Cell Biology 2022Quote: ... Larvae were fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2.5% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M sodium-cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...