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Citations for Polysciences :
301 - 350 of 1066 citations for Ethyl 2 pyrrolidin 2 yl 2 3 dihydro 1 3 thiazole 4 carboxylate hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 100 mm diameter dishes to 90% confluency and were subsequently transfected with 6 μg of pCG1_SARS-2-S plasmid using polyethylenimine (PEI) Max (MW = 40,000KDa, Polysciences, Germany) as transfection reagent ...
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bioRxiv - Microbiology 2022Quote: Leishmania promastigotes were harvested in logarithmic growth phase and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... luciferase encoding reporter plasmid (pTG126) and plasmid encoding Wuhan strain SARS-CoV-2 S using 24 μL polyethylenimine (Polysciences) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using PolyJet™ DNA In Vitro Transfection Reagent (Signagen, #SL100688) or PEI (Polysciences Inc, 23966-2) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: The pcDNA3.4 plasmid containing His-tagged SARS-CoV-2 spike protein was transfected to Expi293T cells using PEI max (Polysciences). After 18–21 h post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated for 24 hours at 37°C before the cells were transfected with 2 µg cDNA coding for hASIC1a (inserted into pcDNA3.1+ vector upstream IRES GFP) using Polyethylenimine (PEI) 25K (Polysciences) in a 1:3 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK 293T cells were transfected with 8 µg expression vectors and 2 µg of third-generation lentiviral packaging system in complex with 24µg PEI (Polysciences) in 1.2 ml of 0.9% NaCl ...
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bioRxiv - Cell Biology 2024Quote: Worms were anesthetized in 0.1 mg/ml tetramisole in 1X M9 buffer on empty NGM plates and mounted on thin 2% agarose pads on glass slides with 0.05 mm Polybead microspheres (Polysciences) for immobilization ...
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bioRxiv - Neuroscience 2021Quote: ... mixed with the transfection reagent polyethylenimine hydrochloride (PEI Max, 24765-1, Polysciences, Inc., Warrington, PA, USA) at a 1:2 ratio (W/V) ...
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bioRxiv - Cancer Biology 2022Quote: ... The transfection was performed with polyethylenimine (PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride, 24765-1, Polysciences) as described previously74 ...
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bioRxiv - Immunology 2024Quote: ... Four milliliters of PEI MAX® (Transfection Grade Linear Polyethylenimine Hydrochloride, MW 40,000, Polysciences, 24765-1) at 1 mg/mL was added ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg polyethylenimines (PEIs, Polysciences) in 50 mL SMM 293T-II medium (Sino Biological Inc. ...
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bioRxiv - Cell Biology 2021Quote: Latex NH2-beads (3 μm diameter, 3.5 x 108 beads/preparation, Polysciences) were activated with 8% glutaraldehyde in 0.5 ml PBS for 120 min under rotation at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... was covalently functionalized with N-(3-Aminopropyl)methacrylamide (APMAm; Polysciences, Warrington, PA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Developmental Biology 2023Quote: Aliquot 15 μL of Concanavalin A (ConA) beads (Polysciences cat#86057-3) and bind to magnet for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... BioMag®Plus Concanavalin A-coated magnetic beads (#86057-3, Polysciences, USA), previously activated with 20 mM HEPES pH 7.9 ...
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bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
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bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
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bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Cutaneous afferents innervating both hind paws of male C57BL/6J mice were labelled with the retrograde tracer Fast Blue (2% w/v in sterile PBS; Polysciences) when animals were aged 9 weeks ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with FACS buffer and fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Warrington, PA) diluted in FACS buffer (fixation buffer) ...
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bioRxiv - Microbiology 2020Quote: Subconfluent 293T cells in a T75 flask were transfected with 15 μg of the SARS-CoV-2 S plasmid using polyethylenimine (Polysciences), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and the slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Microbiology 2020Quote: 293T cells were grown to ~70% confluence and transfected with a mixture of 2 μg of DNA encoding the gRNA and 6 μL of Polyethylenimine (PEI, Polysciences catalogue #24765 ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vector Per2::dLuc using PEI Max (Polysciences). At 48 hours post-transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... transient transfection mixtures were created by mixing 500 ng of plasmid with polyethyleneimine (PEI-MAX, linear 40 kD #24765-2, Polysciences) at 4.12 μg of polyethyleneimine per microgram of DNA in 150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vectors Per2::dLuc using PEI Max (Polysciences). At 48 hours post- transfection ...
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bioRxiv - Cell Biology 2024Quote: ... KIF2B and KIF2C G495A were obtained by transfecting cells with appropriate DNA construct using polyethylene imine (PEI; Polysciences, 23966-2). HeLa kyoto cells stably expressing LAP tagged KIF2B ...
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bioRxiv - Cell Biology 2023Quote: Evaluation of phagocytic uptake was performed using Fluoresbrite® BB Carboxylate Microspheres 1.75µm (Polysciences, 17686-5). Opsonization of the microspheres was performed by incubation in 50% FBS in PBS for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... RAW264.7 cells were incubated with fluorescent microspheres (fluoresbrite carboxylate microspheres with a 0.7 μm diameter, Polysciences) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... mixed with the transfection reagent polyethylenimine (PEI) hydrochloride (PEI Max, 24765-1, Polysciences, Inc., Warrington, PA, USA) at a 1:2 ratio (W/V) ...
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bioRxiv - Microbiology 2020Quote: ... using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences, Inc.), as previously described [54] ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg): PEI (μL) (Polysciences, USA) at 1mg/mL in molecular grade water ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... After washing 3 times in PBS sections were mounted with Aquapolymount (Polysciences Inc.), and coverslipped (Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with plasmids of interest (Table 3) using polyethylenimine (PEI, Polysciences). At 4 h post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.