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Citations for Polysciences :
251 - 288 of 288 citations for Recombinant Human Interleukin 2 Receptor Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vectors Per2::dLuc using PEI Max (Polysciences). At 48 hours post- transfection ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Microbiology 2021Quote: ... one half of the ovaries of each of 6 crickets was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, USA) in 100 mM phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 S gene expression vector (wild type or mutant) in conjunction with p8.9138 and pCSFLW39 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Neuroscience 2019Quote: ... for 10 min on 2% agarose pad and then immobilized using 1.5 μl Polystyrene 0.10 micron microspheres and a coverslip (Polysciences, Inc, Cat# 00876). GCaMP3 imaging was performed on a Zeiss AxioImager microscope using a Q-Imaging Rolera EM-C2 EMCCD camera ...
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bioRxiv - Biochemistry 2020Quote: ... washed in two drops of water and stained 30 s in either 0.75% uranyl formate (UF) or 2% sodium phosphotungstate (NaPT, Polysciences Inc. USA), pH 7.5.
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bioRxiv - Immunology 2020Quote: A microsphere suspension was prepared by diluting 2 μm fluorescent polystyrene microspheres (Fluoresbite® YG Carboxylate Microspheres, Polysciences, 2.5% microsphere suspension) 1:1 (v/v ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Neuroscience 2022Quote: ... The seeded cells were then transfected with a total 2 μg of DNA using polyethylenimine 25 kDa (PEI, Polysciences Inc., Germany) at a 6:1 ratio of PEI to DNA ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 spike gene expression vector in conjunction with lentiviral vectors p8.91 and pCSFLW using polyethylenimine (PEI, Polysciences, Warrington, USA). Pseudotype-containing supernatants were harvested 48 hours post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed and incubated in serum-free MEM media for 2 h prior to addition of 1.75-micron latex beads (Fluoresbrite Carboxylate YG Microspheres, Polysciences, Inc, PA). Cells were incubated with beads at 37°C for an additional 2 h ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted embryos were transferred via a hand drawn glass capillary to a 2 uL drop of M9 buffer containing ∽100 polystyrene beads 20 um in diameter (Polysciences Inc.), sandwiched between two pieces of #1.5 coverglass ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was run twice on a BD Canto II flow-cytometer with 2 μm diameter fluorescent beads (Polysciences, Warminster, PA, USA) as a size and fluorescence standard ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells/mL culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa PEI (Polysciences, 24765-1) per mL culture volume ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Neuroscience 2022Quote: ... mt-GCaMP6f: AAV1) and transfer plasmids were mixed and diluted in saline before mixing with saline-diluted polyethylenimine (PEI, Polysciences #23966-2) and brief vortexing ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The decalcified tibiae were immersed in a 1:1 mixture of 5% potassium dichromate and 2% osmium tetroxide (23310-10, Polysciences, Inc., USA) for 48 h and stained ...
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bioRxiv - Physiology 2024Quote: ... and then transfected with the CYP27C1 construct or a control construct (pCAG-dsRed-2A- GFP) using polyethylenimine (PEI, 23966-2, Polysciences, Warrington, PA). After 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... Calculations of relative fluorescence per cell were done by normalizing red chlorophyll fluorescence per cell to 2 μm reference fluorescent beads (catalog no. 18604; Polysciences, Warrington, PA, USA) as previously described [72] ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were then washed for 10 minutes in PBS (3 times) and stained with a 1:1 mixture of 2% aqueous osmium tetroxide (cat# 23310-10, Polysciences, Inc., Warrington, PA, USA) and 5% potassium dichromate for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: Under isoflurane anaesthesia (1.5-2%) a small incision was made at the Achilles tendon and the tracer Fast Blue (FB, Polysciences, 1.5% in 3 µL PBS) was injected into the soleus muscle and Alexa Fluor 488-conjugated Cholera Toxin subunit B (CTB 488 ...