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Citations for Polysciences :
251 - 300 of 339 citations for 7 Fluoro 4 hydroxy 3 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... All whole digestive tract was removed and fixed in PBS and 4% electron microscopic-grade paraformaldehyde (Polysciences, USA) for 35 min ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... TrpA1-KI-Flag and TrpA1 mutant (different days as specified in legend) were fixed in 4% Formaldehyde (Polysciences) in 1XPBS for 2 hours under room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic Müllerian and adult oviducts were dissected in PBS and fixed in 4% w/v PFA/PBS (Polysciences) for 30 minutes at room temperature followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... At 18 DIV Cells were fixed using 4 % PFA for 15 min and mounted on glass slides using Aqua-Poly/Mount (Polysciences Inc., Eppelheim). The experimenter was blinded to all the conditions ...
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bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
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bioRxiv - Bioengineering 2019Quote: Neurons were fixed in methanol-free 4% formaldehyde ultra-pure EM grade (Polysciences, Inc., cat. no. 18814-10) + 4% sucrose in PBS for 20 min at room temperature and permeabilized with 0.2% Triton X-100 for 5-10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg): PEI (μL) (Polysciences, USA) at 1mg/mL in molecular grade water ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Immunology 2023Quote: ... 0.5 mM spermidine in 1X protease inhibitor buffer) and incubated with activated concanavalin A (ConA) coated magnetic beads (Polysciences - 86057-3) in PCR strip tubes at room temperature for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... the fly abdomens were opened in PBS and subsequently fixed for 20 min with 4% PFA in PBS (Polysciences). Aqua Polymount (Polysciences ...
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bioRxiv - Microbiology 2020Quote: ... infected cells then were fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Bioengineering 2019Quote: ... agarose RoCs were fixed in 4% paraformaldehyde in 0.1 M phosphate buffered saline (pH 7.4) (Polysciences, Warrington Pa., USA) for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: Cells destined for immunofluorescence were washed with PBS once and fixed using 4% wt/vol paraformaldehyde (PFA, Polysciences Inc.) for 20min ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
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bioRxiv - Microbiology 2019Quote: ... were co-transfected with 3 µg Gibson Assembly product DNA plus 330 ng pHEF-VSV-G using polyethylenimine (Polysciences, Inc., Warrington, PA) at a ratio of 1 µg total DNA to 4 µg polyethylenimine in 800 µl of 150 mM NaCl [72] ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Physiology 2024Quote: ... The balloon was inflated after the first diagonal branch of the LAD in each animal and 3 ml polystyrene microspheres (Polybead, 90 μm, Polysciences, Warrington, PA) were slowly injected via the lumen of the angioplasty catheter ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...