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Citations for Polysciences :
251 - 300 of 1151 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Transfection solution was prepared by combining 6 µL (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 200 µL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Biochemistry 2020Quote: ... Contrasting for TEM was done by embedding the grids in 1.8% methyl cellulose and 0.4% uranyl acetate (Polysciences, Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...
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bioRxiv - Bioengineering 2020Quote: ... After washing with acetic acid (1%, Fluka) samples were incubated with safranin O (0.1%, 15 min, Polysciences) to stain proteoglycans in red ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: Cells for ICC were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Pathology 2022Quote: ... Slides were placed in phosphomolybdic acid (Polysciences) for two minutes ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... with 1 mg of total DNA and 4 mg of PEI (Polysciences, 24765) per 1 liter of cells at a density of 1.5-2 x 106 cells/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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bioRxiv - Microbiology 2021Quote: ... spores were harvested in KK2 buffer and fixed with 2% paraformaldehyde with 2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Live animals were immobilized with 0.01% tetramisole and 0.1% tricaine in M9 buffer and mounted on 2% agarose pads or with 0.05 μm Polybead microspheres (Polysciences) diluted 1:2 in water and mounted on 5% agarose pads ...
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bioRxiv - Molecular Biology 2022Quote: ... 293T media was changed and the cells were transfected using 1mg/ml of polyethylenimine (PEI) (Polysciences, 23966-2).
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20U/ml) for 2 min at 35°C followed by 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% formaldehyde (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 4µg pMDG.2 plasmid and 4µg psPAX plasmid by using the transfection reagent Polyethylenimine (PEI) (Polysciences #23966-100) at a PEI:DNA ratio of 4:1 ...
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bioRxiv - Immunology 2022Quote: ... HEK293E cells were transfected at a density of 106 cells/mL using 0.5 mg/mL of pTT3-RBD-HISAVI DNA and 2 mg/mL filter sterilized pH 7.0 Polyethylenimine (PEI) MAX (40,000 MW, Polysciences). Six days later ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by 2 minutes incubation of the slices in 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences). rinsed 5 × 2 min in chilled buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20 U/ml) for 2 min at 35°C followed by 0.15 M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% paraformaldehyde (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Immunology 2023Quote: Polybead amino 6 µm-diameter microspheres (Polysciences) were prepared to covalently couple rCD6 or rICAM1 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were properly oriented and embedded in Poly/Bed® 812 (Dodecenyl Succinic Anhydride, Nadic Methyl Anhydride, Poly/Bed 812 Resin and Dimethylaminomethyl, Polysciences). Thin (300 nm ...
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bioRxiv - Immunology 2022Quote: ... 1 mg of plasmid was mixed with 4 mg of polyethyleneimine (PEI; PolySciences Inc.) in Opti-MEM cell culture medium (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 60mL ice-cold 4% paraformaldehyde (PFA) (ref # 00380-1, Polysciences, Warrington, PA) in 0.1 M phosphate buffer (80 mM Na2HPO4 and 19 mM NaH2PO4 ...
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bioRxiv - Physiology 2024Quote: ... Hearts were then embedded using a JB-4 kit (Catalogue number 00226-1, Polysciences). Briefly ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...