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Citations for Polysciences :
251 - 300 of 383 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Material collected for microscopy was fixed in 4% v/v acrolein (Polysciences, New Orleans, Louisiana, USA) in 1X PIPES buffer (50 mmol/L PIPES ...
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bioRxiv - Biophysics 2020Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Cell Biology 2022Quote: ... Larvae were fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2.5% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M sodium-cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Cell Biology 2021Quote: ... Streptavidin-conjugated Yellow-Green latex beads (2 μm diameter, 5 x 108 beads/preparation, Polysciences) were washed with 1% BSA in PBS and incubated with Biotin-SP (long spacer)-conjugated Fab fragments of goat-anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... and covers were mounted with Aqua-Poly/Mount solution (cat#18606-5 from Polysciences Europe) on glass slides ...
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bioRxiv - Neuroscience 2024Quote: ... 5 days after having injected HVC with the retrograde tracer Fast Blue (150nl/HVC, Polysciences) the brain was perfused with 4% paraformaldehyde (PFA) ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free formaldehyde (FA) (Polysciences, 040181) during 2 hours at RT and then rinsed in PBSDT (PBS 1x ...
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bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Segments of 0.5cm from flexible branches were fixed with 4% w/v acrolein (Polysciences, Warrington, PA, USA) in a modified piperazine-N ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Developmental Biology 2024Quote: ... Testes or cauda epididymis were fixed at 4°C in Bouin’s solution (Polysciences, Inc., Warrington, PA, USA) and were processed for paraffin embedding ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μg each of DNA constructs respectively were used with polyethylenimine (PEI; Polysciences, Eppelheim, Germany) as previously described (8 ...
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bioRxiv - Physiology 2020Quote: ... animals were post-fixed with 1% aqueous solution of osmium tetroxide (Polysciences Europe GmbH 0972B-5) and 1.5% potassium ferrocyanide (Sigma Aldrich ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Adeno-associated viruses (AAVs) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... DAPI for 5 minutes and then cover slipping the tissue sections with Aqua-Poly mount (Polysciences). CD25+ cells were detected after the TBS wash step by blocking with 3% H2O2 for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl (10^10 microspheres/mL) of Fluoresbrite® Multifluorescent Microspheres 0.2 µm (PolySciences 24050-5) were spread onto a bilayer plate using sterile glass beads and dried for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: Evaluation of phagocytic uptake was performed using Fluoresbrite® BB Carboxylate Microspheres 1.75µm (Polysciences, 17686-5). Opsonization of the microspheres was performed by incubation in 50% FBS in PBS for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS twice for 5 min and mounted with Aqua PolyMount medium (PolySciences). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... Day-one adults were mounted with 100 nm polystyrene beads (Polysciences #00876-15; 5-fold dilution) on an agarose pad ...
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bioRxiv - Neuroscience 2024Quote: ... following previously published protocol.42 Triple transfection was performed with Transporter 5 Transfection Reagent (Polysciences 26008). AAVs were harvested 72 hours post-transfection and purified on iodixanol gradient ...
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bioRxiv - Cancer Biology 2021Quote: ... All whole digestive tract was removed and fixed in PBS and 4% electron microscopic-grade paraformaldehyde (Polysciences, USA) for 35 min ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... TrpA1-KI-Flag and TrpA1 mutant (different days as specified in legend) were fixed in 4% Formaldehyde (Polysciences) in 1XPBS for 2 hours under room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic Müllerian and adult oviducts were dissected in PBS and fixed in 4% w/v PFA/PBS (Polysciences) for 30 minutes at room temperature followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... At 18 DIV Cells were fixed using 4 % PFA for 15 min and mounted on glass slides using Aqua-Poly/Mount (Polysciences Inc., Eppelheim). The experimenter was blinded to all the conditions ...
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bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Cancer Biology 2024Quote: After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Microbiology 2024Quote: ... After 1-hour incubation at 37°C the treated and the untreated live HEK293 cells expressing HBc and the untransfected HEK293 cells were washed and fixed with 0.1 M sodium phosphate buffer pH 7.4 containing 4% paraformaldehyde (EM grade, Polysciences) and 1% sucrose for 10-20 min at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... The transfection solution was prepared by combining 5 μL of 1 mg/ml polyethylenimine Max solution (Polysciences) and a plasmid mixture consisting of 200 ng ssHA-FLAG-NTS1 and 500 ng alkaline phosphatase (AP)-tagged TGF-α (AP-TGFα ...
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bioRxiv - Neuroscience 2020Quote: ... We mounted sections on coated slides and dipped slides in Ilford K.5 nuclear tract emulsion (Polysciences, 1:1 dilution in double-distilled water ...
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were freeze substituted in 0.1– 5% uranyl acetate in acetone and embedded in Lowicryl HM20 (Polysciences) with automated temperature control (Leica Microsystems EM-AFS2) ...
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bioRxiv - Immunology 2021Quote: ... the fly abdomens were opened in PBS and subsequently fixed for 20 min with 4% PFA in PBS (Polysciences). Aqua Polymount (Polysciences ...
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bioRxiv - Microbiology 2020Quote: ... infected cells then were fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... A minimum of 4 weeks after viral injections fluorophore-conjugated retrograde tracers (Dextran 10,000MW, AlexaFluor 488 and 568, Invitrogen; FastBlue, Polysciences) were injected bilaterally into Area X ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...