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Citations for Polysciences :
251 - 300 of 438 citations for 4 2 Trimethylsilyl Ethyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... An aqueous 10µg/ml stock of linear 25kDa PEI (Polysciences, catalogue number 23966-2) was used for the transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µl of 6-µm-diameter polystyrene MB suspension (containing 6.3 × 106 beads, Polysciences), and 1 µl of PBS containing 30% Healon was injected into the anterior chamber using a 32-gauge needle (Hamilton Company ...
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bioRxiv - Cancer Biology 2022Quote: ... and 19.5 μg of psPAX2 were transfected using polyethylenimine (1μg/μl, Polysciences # 23966-2) into 293T cells plated in 6-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... The transfection was performed accordingly: 2 μl polyethylenimine (PEI) 25K (1 mg/ml; Polysciences) was mixed with each μg of plasmid in Opti-MEM medium (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... All whole digestive tract was removed and fixed in PBS and 4% electron microscopic-grade paraformaldehyde (Polysciences, USA) for 35 min ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... TrpA1-KI-Flag and TrpA1 mutant (different days as specified in legend) were fixed in 4% Formaldehyde (Polysciences) in 1XPBS for 2 hours under room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic Müllerian and adult oviducts were dissected in PBS and fixed in 4% w/v PFA/PBS (Polysciences) for 30 minutes at room temperature followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... At 18 DIV Cells were fixed using 4 % PFA for 15 min and mounted on glass slides using Aqua-Poly/Mount (Polysciences Inc., Eppelheim). The experimenter was blinded to all the conditions ...
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bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Microbiology 2024Quote: ... After 1-hour incubation at 37°C the treated and the untreated live HEK293 cells expressing HBc and the untransfected HEK293 cells were washed and fixed with 0.1 M sodium phosphate buffer pH 7.4 containing 4% paraformaldehyde (EM grade, Polysciences) and 1% sucrose for 10-20 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... the fly abdomens were opened in PBS and subsequently fixed for 20 min with 4% PFA in PBS (Polysciences). Aqua Polymount (Polysciences ...
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bioRxiv - Microbiology 2020Quote: ... infected cells then were fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... A minimum of 4 weeks after viral injections fluorophore-conjugated retrograde tracers (Dextran 10,000MW, AlexaFluor 488 and 568, Invitrogen; FastBlue, Polysciences) were injected bilaterally into Area X ...
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bioRxiv - Cancer Biology 2024Quote: ... Grids were stained in uranyl-oxalate at pH 7 and contrasted in a mixture of 4% uranyl acetate (Polysciences) and 2 % methyl cellulose (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: Guts from adult female flies were dissected in PBS and immediately fixed in 4% formaldehyde (FA, EM grade, Polysciences) diluted in PBS for 1h at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: Third-generation VSV.G pseudotyped HIV-1 lentiviruses were produced by polyethylenimine (PEI, Polysciences #23966-2)-transfection of HEK293T cells and packaged with VSVG-coats using established methods ...
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bioRxiv - Microbiology 2021Quote: ... highly synchronized infected RBCs were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Streptavidin-conjugated Yellow-Green latex beads (2 μm diameter, 5 x 108 beads/preparation, Polysciences) were washed with 1% BSA in PBS and incubated with Biotin-SP (long spacer)-conjugated Fab fragments of goat-anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
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bioRxiv - Physiology 2023Quote: ... Sections were picked up on 1 x 2-mm formvar-coated copper slot grids (Polysciences) and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Neuroscience 2022Quote: ... 4.1 µg of VSVG and 110 µL of Polyethylenimine (1 µg/µL; Polysciences, 23966-2). This solution was incubated for 10 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... bacmid (1 µg/mL culture volume) was mixed with 2 µg PEI 25K (Polysciences, Inc.) per µg bacmid in 200 µL warm PBS and incubated at RT for 30 mins ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with one or more constructs using polyethylenimine (PEI, Polysciences, Inc.; Warrington, PA, USA, 23966-2).
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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Brain-wide mapping of oligodendrocyte organization and oligodendrogenesis across the murine lifespanbioRxiv - Neuroscience 2024Quote: ... the tissue was washed 4 times with 20mM PB buffer and sections were mounted onto slides with Aqua Polymount (18606, Polysciences) and allowed to dry for 24-48 hours at 4°C prior to imaging ...
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bioRxiv - Biophysics 2024Quote: ... diluted 1:100 in nanopure water were diluted 1:10 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in nanopure water and spin coated (WS-650 ...