Labshake search
Citations for Polysciences :
201 - 250 of 383 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Transfection complexes were prepared by mixing plasmid DNA with polyethyleneimine (PEI Max 40k, Polysciences Inc., Warrington, PA) in a 1:4 mass ratio ...
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bioRxiv - Immunology 2021Quote: ... PEI precipitation was performed by incubating the plasmids with 420 μg PEI (molecular weight 25,000, Polysciences, Inc) in 10 mL Opti-MEM (Life technologies ...
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bioRxiv - Microbiology 2020Quote: ... triple plasmid transfection of 293 adherent cells was performed with a polyethylenimine (PEI)-MAX (Polysciences, MW, 40,000) to DNA ratio of 3:1 (a mass-per-mass ratio) ...
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bioRxiv - Biophysics 2020Quote: ... plasmids encoding DISP1-A-NNN-SBP were transfected into the HEK293F cells using linear polyethylenimines (PEIs, Polysciences). Both DISP1-A and DISP1-A-NNN were purified essentially as previously described[17] ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) or TransIT-293 (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... and 8µg custom generated lentiviral CMV-PR or 2x/4x/6xPRE-GFP plasmids using PEI (Polysciences, #23966). Medium was refreshed after 24 hours ...
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bioRxiv - Biophysics 2023Quote: ... A ratio of 1.2 µg Plasmid DNA per 106 cells and 40 kDa linear PEI MAX (Polysciences) transfection reagent was used for protein expression ...
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bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
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bioRxiv - Molecular Biology 2023Quote: Equal numbers of HEK293T cells were seeded and the different plasmids were transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T cells were co-transfected with 100 ng of corresponding plasmids using linear PEI (Polyethyleneimine, Polysciences). 24h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids expressing Fcα–J and FcRL4 was co-transfected into the HEK293F cells by polyethylenimine (Polysciences). The cells were cultured at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding HLA class I/II alleles [46] were transfected in HEK293T cells with PEI MAX (Polysciences).
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Microbiology 2023Quote: ... SIRT3 and scramble control under U6 promoter together with helper plasmid using PEI Max 40000 (Polysciences USA). Next day transfection medium was removed ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids were diluted in DMEM and incubated for 20 min at RT with Polyethylenimine (PEI, 23966, Polysciences) at a ratio 1:3 DNA:PEI ...
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bioRxiv - Microbiology 2024Quote: HIV-1 Gag-iFRET plasmid DNA was transfected into HEK293T cells using a PEI transfection method (Polysciences) and into Jurkat cells using Lipofectamine LTX transfection reagent (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were transiently transfected with ADAMTS6 WT or ADAMTS6 EA expression plasmids using PEI MAX (24765; Polysciences) and were co-cultured with Fbn1-/- mouse embryo fibroblasts (MEFs ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
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bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were transiently transfected with 2mg expression plasmid per 1 liter using the Polyethylenimine (PEI) MAX (Polysciences) at a density of 1.0 x 106 cells/ml in fresh Union 293 medium ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
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bioRxiv - Immunology 2022Quote: ... The S2P or S6P pCMV plasmids were used to transiently transfect HEK293F cells with polyethylenimine (PEI) (Polysciences, #24765). The recombinant S6P was affinity purified from the cell supernatant by using StrepTactin resin (IBA) ...
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bioRxiv - Cell Biology 2019Quote: ... MDCK-II cells were transfected with 1 μg plasmid DNA per 106 cells using either polyethlyenimine (Polysciences Inc) or Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2021Quote: ... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 Freestyle™ were transiently transfected at a density of 2.5 106 cells/mL in 100mL Freestyle medium (Thermo-Fisher) by addition of 150 μg plasmid and 1.8 mL of linear polyethylenimine (PEI, 0.5 mg/ml) (Polysciences). After 24h ...
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bioRxiv - Immunology 2020Quote: ... pEE12.4-RBD-SpyTag-Histag plasmids were transfected with PEI (PEI MAX-Transfection Grade LinearPolyethylenimine Hydrochloride, Polysciences 24765-1) into 293F cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids for these proteins were co-transfected with AnkR-GFP in HEK293T cells using PEI Max (Polysciences, 24765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Physiology 2022Quote: ... 16 μg AAV-U6-sgRNA-TBG-Cre plasmid and 144 μl of 1 mg/ml Polyetherimide (PEI) (Polysciences) was mixed in 9 ml OptiMEM (Thermo) ...
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bioRxiv - Bioengineering 2022Quote: ... 750 ng of ABEs and 250 ng of sgRNA expression plasmids were co-transfected using polyethyleneimine (PEI, Polysciences) following the manufacturer’s recommended protocol ...
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bioRxiv - Biochemistry 2023Quote: HEK cells were transiently transfected at 80% confluency with CibN and CryC plasmids using polyethylenimine (PEI) (Polysciences, Inc.). Plasmids and PEI were diluted in Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with 5 µg plasmid DNA and 15 µg Polyethylenimine (PEI 25K™, Polysciences, 23966-100), both previously prepared in OptiMEM (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
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bioRxiv - Biochemistry 2024Quote: HELBKO HEK293T cells were transfected with plasmids encoding pcDNA5/FRT/TO-SFB-HELB variants using PEI (Polysciences, Inc). After 24 hours ...