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Citations for Polysciences :
201 - 250 of 980 citations for 6 BROMO 4 4 DIETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Bioengineering 2019Quote: ... agarose RoCs were fixed in 4% paraformaldehyde in 0.1 M phosphate buffered saline (pH 7.4) (Polysciences, Warrington Pa., USA) for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: Cells destined for immunofluorescence were washed with PBS once and fixed using 4% wt/vol paraformaldehyde (PFA, Polysciences Inc.) for 20min ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Immunology 2023Quote: ... 3 μl of linear PEI (1mg/ml, Cat. No. 23966-1, Polysciences) transfection reagent was used per 1 μg of plasmid DNA ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... or PEI Max (3 µg PEI per 1 µg DNA) (Polysciences 24765) (for substrate only transfection for investigating endogenous PS (Fig 5 & 6) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Developmental Biology 2019Quote: Flies of both genders were raised as described above and larvae from second instar to pre-pupal stages collected and dissected directly into fresh 4% EM grade paraformaldehyde solution (from a 16% stock. Polysciences) in PBS with 0.3% TritonX-100 then incubated for 25 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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bioRxiv - Microbiology 2019Quote: ... Pandoravirus massiliensis virions were then placed for 1h in a mix of LR-White resin 100% (Ref. 17411, MUNC-500; Polysciences) and ethanol 96% in a 2:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl polyethylenimine (PEI) 25K (1mg/ml; Polysciences, 23966-1) per μg of plasmid DNA were mixed with pre-warmed Opti-MEM media (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al. 2020) with 24 μg linear PEI (Polysciences Inc., Warrington, PA) for 6 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and 10 ng of the TetR reporter plasmid pTS1017) was added into 300 μL DMEM and mixed with 4 μg of polyethyleneimine (Polysciences Inc.). The transfection mixture was vortexed and incubated for 20 min before adding into the inner 60 wells of the cell culture plate ...
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bioRxiv - Bioengineering 2020Quote: The pore size of mineralized collagen and mineralized collagen-amnion scaffolds was analyzed following a JB-4 (Polysciences, Inc., Warrington, PA) embedding procedure (9,25) ...
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bioRxiv - Physiology 2021Quote: ... The 4-μm bone sections were stained with the von Kossa method and counterstained with tetrachrome (Polysciences Inc., Warrington, PA, USA). The 8-μm sections remained unstained to measure fluorochrome-based indices of bone formation.
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bioRxiv - Neuroscience 2019Quote: ... We then sterilized 1- and 6-µm diameter polystyrene microbeads (Polysciences, Cat#: 07310-15 and 07312-5) as noted in Cone et al. ...
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following by curing the resin overnight at 4°C in a plastic embedding mold (Cat no.-15899-50, Polysciences Europe GmbH, Germany). Polymerized sample blocks were further hardened by overnight baking at 70°C.
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Cell Biology 2019Quote: The tail fin was amputated up to the level of the notochord and larvae were fixed 20-24 hours later in 1ml of 4% ethanol-free formaldehyde (Polysciences, Warrington, PA) in phosphate-buffered saline (PBS ...
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bioRxiv - Plant Biology 2020Quote: ... Dehydration was performed in an acetone series, followed by treatment with propylene oxide (SERVA, Heidelberg, Germany) at 4°C and embedding in Spurr’s resin (PolySciences, Niles, IL, USA). The embedded specimens were sectioned with a Reichert-Jung Ultracut E (Reichert-Jung Optical Company ...
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bioRxiv - Cell Biology 2022Quote: T24 cells were fixed in 2% glutaraldehyde and 2% paraformaldehyde at 4°C overnight and then postfixed in 2% osmium tetraoxide for 1.5 h at 4°C followed by dehydration with ethanol and embedding in Spurr resin (Polysciences Inc., Warrington, PA). Ultrathin sections of the samples were prepared using Ultramicrotome (Leica EM UC7 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...
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bioRxiv - Cell Biology 2024Quote: Midguts were dissected in 1X PBS (Oxoid, BR0014G) and fixed for 20 minutes at room temperature in fresh 4% methanol-free paraformaldehyde (Polysciences, 18814-10) diluted in PBS ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Immunology 2023Quote: Polybead amino 6 µm-diameter microspheres (Polysciences) were prepared to covalently couple rCD6 or rICAM1 ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...