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Citations for Polysciences :
151 - 200 of 212 citations for Siglec 10 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Slides were first stained in Weigert’s Iron Hematoxylin solution for 10 min at room temperature (Polysciences Inc., Warrington, PA). This was followed by 2 min wash in tap water ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Developmental Biology 2021Quote: ... elegans larvae were mounted on a 10% agarose pad in a solution of 100 nm polystyrene microbeads (Polysciences Inc.) to stop worm movement ...
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bioRxiv - Neuroscience 2022Quote: ... Worms were mounted on a 10% agarose pad with 1.6 µl of a 1:1 mixture of polystyrene beads (Polybead, catalog #00876-15, Polysciences) and 30 µM Muscimol (catalog #195336 ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: The MPs comprised Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1.00 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in aqueous suspension without sodium azide ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 48 hours at 37°C after which the media was removed and 150 uls of 10% formaldehyde (Polysciences) was added to inactivate the virus ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed 3 times for 30 mins with PBS then incubated through a cryoprotective series of sucrose solutions of increasing concentration from 10-50% for ≥3 hours each before embedding into OCT compound (Tissue Tek) in disposable cryomolds (Polysciences) and frozen in an isopentane bath cooled by liquid nitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Neuroscience 2020Quote: ... One-day-old adults from these strains were mounted on a 10% agarose pad with 1.6 µL of a mixture containing equal measures of polystyrene beads (Polybead, Polysciences Inc.) and 30 mM muscimol (MP Biomedicals) ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and the slides were mounted with Aqua-Poly/Mount (Polysciences).
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Neuroscience 2019Quote: ... The Gorilla Glue Company) to a microscope slide in 2µL 0.05um polybeads (2.5% solids w/v, Polysciences, Inc. #15913-10) plus 3µL of M9 buffer and imaged under a coverslip ...
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bioRxiv - Immunology 2020Quote: Cell numbers per tissue were determined by mixing 20 µl of each single cell suspension into a 96-well plate with 50 μl of 8.4 × 104 Fluoresbrite Carboxylate YG 10 µm microspheres/ml (Polysciences Inc.) and 180 μl staining media (dPBS + 2% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... and then transfected with 0.75 μg of an equimolar mixture of the three third-generation packaging plasmids (pMD2.G, psPAX2, pMDLg/pRRE) and 0.75 μg of LSR vectors using 10 μl of polyethylenimine (PEI, Polysciences #23966) and 200 μl of cold serum free DMEM ...
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bioRxiv - Microbiology 2019Quote: ... 3×105 cells/well (12-well plate) or 2×106 (10-cm dish) and transfected on the following day using polyethylenimine (PolySciences).
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were dissected in 8-10 μL of Shelton’s Growth Medium (Edgar and Goldstein, 2012) and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA with the amount of 10 μg was mixed with 30 μg of linear polyethyleneimine (PEI) (23966-100; Polysciences) in 1 ml of serum-free DMEM ...
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bioRxiv - Cancer Biology 2023Quote: Poly(methyl methacrylate-co-methacrylic acid) (PMMA-MA; 10% methacrylic acid, MW ∼100,000 Da, 17913-500) was from Polysciences (Germany), organic solvents were obtained from Carl Roth (Austria) ...
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bioRxiv - Neuroscience 2023Quote: ... were used The slides were again washed and stained with DAPI (Thermo-Fisher, 300 nM) for 10 min prior to mounting with Aqua PolyMount medium (PolySciences).
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mm dishes of HEK293T cells (∼80% confluent) were transfected with 10 μg of plasmid using 60 μg of linear Polyethylenimine 25 kDa (23966, Polysciences Inc) mixed in 2 mL Opti-MEM (31985070 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed 3 × 30 mins in PBS and then immersed through a gradient of sucrose solutions from 10%-50% at 4°C for > 4 hrs each before being embedded in cryomolds (Polysciences 18646A) using OCT (Sakura Finetek ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Microbiology 2022Quote: ... mice were fasted for two hours and then given a single dose of 1×106 10 μm Fluoresbrite® YG carboxylate microspheres (Polysciences) via oral gavage ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then dehydrated with increasing concentrations of ethanol (50-100%),infiltrated with propylene oxide for 10 min and embedded with a Poly/Bed 812 kit (Polysciences, USA) and polymerized for 18 hours at 60°C ...
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bioRxiv - Bioengineering 2024Quote: ... negative staining was performed by depositing for 10 minutes with the gird membrane side down on a drop of 1% (w/v) uranyl acetate (Polysciences, USA). The remaining solution on the grid was removed using filter paper (Whatman ...
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bioRxiv - Molecular Biology 2024Quote: ... was co-transfected with a pcDNA5/FRT/TO plasmid containing the gene of interest in a 1:10 ratio using polyethylenimine “MAX” (Polysciences, 24765). After medium exchange 6 h post-transfection ...
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bioRxiv - Cell Biology 2024Quote: HEK 293T cells on 15 x cm2 dishes were transfected with 3.33 mg of each construct for a final 10 μg of total DNA using 25 μL polyethylenimine (PEI; Polysciences, Warrington, PA). Cells were lysed mechanically in a hypotonic solution (20 mM Tris-HCl (pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Developmental Biology 2022Quote: Worms were mounted without anesthetic on 10% agarose pads in a 1:1 solution of M9 buffer and 0.1 μM polystyrene beads (Polysciences, Inc., Warrington, PA) essentially as described (Rosu and Cohen-Fix 2020) ...
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bioRxiv - Cancer Biology 2022Quote: Calculation of absolute cell number was performed by adding to each vial a fixed number (10,000) of nonfluorescent 10 μm polybead® carboxylate microspheres (Polysciences, IL, USA) according to the formula ...
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bioRxiv - Bioengineering 2022Quote: PEG-αMA hydrogels were fabricated at 17.5 wt% with a 0.375 molar ratio of 70:30 DTT:MMP2-degradable as described above and swollen overnight in PBS with 2.2 mM LAP and 10 µM methacryloxyethyl thiocarbamoyl rhodamine B (Polysciences Inc., Warrington, PA). Swollen hydrogels were exposed to 10 mW cm−2 365-nm light for five minutes through a chrome-on-quartz photomask with spatially patterned opaque and transparent regions ...
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bioRxiv - Immunology 2023Quote: 50,000 of thymocytes from Satb1+/Venus mice were resuspended in 0.1mL RPMI-1640 medium (10% FBS) and were mounted on a poly-L-lysine-coated glass slide (Polysciences, Inc., REF: 26414) and incubated at 37 °C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: HEK293T cells cultured in DMEM with 10% Fetal Calf Serum were transfected using linear polyethylenimine (MW ∼25,000, Cat#23966 Polysciences Inc., Warrington, PA), 40 µg/ 10 cm dish and plasmid DNA ...