Labshake search
Citations for Polysciences :
151 - 200 of 291 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: Slices were washed 3 times for 10 minutes in PBS and mounted with Aqua-Poly/Mount (Polysciences). Images were taken using a Zeiss LSM780 confocal microscope and images were processed using ImageJ software ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.4 mg each of TCR and CD3 DNA were mixed with 3 mg of PEI MAX (Polysciences) and added to 0.8 L of HEK293F cells grown in suspension in FreeStyle 293 media (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell counts were calculated by using 15 μm polystyrene microbeads (#18328-5, Polysciences).
-
bioRxiv - Physiology 2021Quote: ... for 5 min at 22 °C and mounted in Mowiol 4–88 (Polysciences) with DABCO (25 mg ml−1 ...
-
bioRxiv - Neuroscience 2020Quote: ... we used FastBlue (5% in distilled water, 17740-1, Polysciences, Inc, Warrington, PA), fluorescent latex microspheres (red retrobeads IX or green retrobeads IX ...
-
bioRxiv - Cell Biology 2021Quote: ... or into a bead slurry containing 20 µm polystyrene beads (Polysciences - #18329-5) in M9 + 0.5% (w/v ...
-
bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfected using the Transporter 5 transfection reagent (Cat#: 26008, Polysciences) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
-
bioRxiv - Neuroscience 2020Quote: ... Finally sections were subsequently washed with PBS (3 x 10 min) and mounted in Aqua/polymount (Polysciences, 18606).
-
bioRxiv - Neuroscience 2024Quote: ... The dyes injected included fluorescent and other tracers: Fast Blue (FB, case AT, 3% dilution, 2 µl; Polysciences), Diamidino Yellow (DY ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 μg DNA/ml transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
-
bioRxiv - Developmental Biology 2022Quote: ... Exogenous plasmid DNA was transfected using Transporter™ 5 transfection reagent (Polysciences, PA, USA). Co-transfection of siRNA with plasmid DNA was performed using jetPRIME® transfection reagent ...
-
bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293T cells were performed using the Transporter 5 Transfection Reagent (Polyethylenimine; Polysciences) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
-
bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
-
bioRxiv - Immunology 2024Quote: ... paraffin-embedded livers were sectioned at 5 µm thickness and stained with hematoxylin (Polysciences) and eosin (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
-
bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...
-
bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
-
bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
-
bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
-
bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
-
bioRxiv - Immunology 2023Quote: ... One coverslip was activated with 3-aminopropyltriethoxysilane using 0.1 M NaOH (Macron Fine Chemicals) and 0.05% glutaraldehyde (Polysciences Inc.) and the other coated with dichloromethylsilane (Sigma) ...
-
bioRxiv - Biophysics 2024Quote: Standard microplastic concentrations were created by an initial dilution of commercial bead samples of known concentration (2.5% w/v 3 µm PS beads, Polysciences; 10% w/v 4 µm PMMA beads ...
-
bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Streptavidin-conjugated Yellow-Green latex beads (2 μm diameter, 5 x 108 beads/preparation, Polysciences) were washed with 1% BSA in PBS and incubated with Biotin-SP (long spacer)-conjugated Fab fragments of goat-anti-mouse IgG (H+L ...
-
bioRxiv - Cancer Biology 2022Quote: ... and covers were mounted with Aqua-Poly/Mount solution (cat#18606-5 from Polysciences Europe) on glass slides ...
-
bioRxiv - Neuroscience 2024Quote: ... 5 days after having injected HVC with the retrograde tracer Fast Blue (150nl/HVC, Polysciences) the brain was perfused with 4% paraformaldehyde (PFA) ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2020Quote: ... MLV-GagPol/pHIV 8.1 and pHIT VSVg at a ratio of 2:3:1 for 48 h using polyethylenimine (Polysciences, Germany). 293T supernatant was collected and filtered through a 0.2-μm filter before being used to transduce MDCK II cells ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected with BirAGFP-KIF13B Tail 2 or Tail 3 constructs (designated C2 and C3, respectively, in (Serra-Marques et al., 2020)) using Polyethylenimine (PEI; Mw 2500; Polysciences) at a 3:1 PEI:DNA ratio (w/w) ...
-
bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
-
bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
-
bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
-
bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
-
bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
-
bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
-
bioRxiv - Immunology 2024Quote: We have previously created FLP using purified fungal carbohydrates which we can associated with amine-coated polystyrene 3 μM microspheres (Polysciences) using either chemical conjugation or adsorption57,58 ...
-
bioRxiv - Molecular Biology 2024Quote: HEK293T cells were transfected with 3 µg pcDNA3 HA-KBTBD4 plasmid and 3 µg pcDNA3 CoREST-FLAG (full-length or truncated) using PEI MAX transfection reagent (Polysciences) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: HEK293T cells were transfected with 2 µg pcDNA3 HA-KBTBD4 plasmid (mutant or WT) and with or without 3 µg pcDNA3 CoREST-FLAG (full-length or truncated) using PEI MAX transfection reagent (Polysciences) according to the manufacturer’s protocol ...