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Citations for Polysciences :
151 - 200 of 231 citations for 4 Nitrophenyl beta D glucuronic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Plant Biology 2021Quote: Material collected for microscopy was fixed in 4% v/v acrolein (Polysciences, New Orleans, Louisiana, USA) in 1X PIPES buffer (50 mmol/L PIPES ...
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bioRxiv - Biophysics 2020Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Cell Biology 2022Quote: ... Larvae were fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2.5% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M sodium-cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Synthetic Biology 2024Quote: ... The 4 plasmids were incubated for 15 minutes with 5 μL of polyethylenimine (PEI, Polysciences #23966) before transfection ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free formaldehyde (FA) (Polysciences, 040181) during 2 hours at RT and then rinsed in PBSDT (PBS 1x ...
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bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Segments of 0.5cm from flexible branches were fixed with 4% w/v acrolein (Polysciences, Warrington, PA, USA) in a modified piperazine-N ...
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bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed 4 x 5 min in PBS and mounted with Aqua-Poly/mount (Polysciences, 18606). STED images were acquired by sequential acquisition of each channel ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Developmental Biology 2024Quote: ... Testes or cauda epididymis were fixed at 4°C in Bouin’s solution (Polysciences, Inc., Warrington, PA, USA) and were processed for paraffin embedding ...
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bioRxiv - Cancer Biology 2021Quote: ... All whole digestive tract was removed and fixed in PBS and 4% electron microscopic-grade paraformaldehyde (Polysciences, USA) for 35 min ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... TrpA1-KI-Flag and TrpA1 mutant (different days as specified in legend) were fixed in 4% Formaldehyde (Polysciences) in 1XPBS for 2 hours under room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic Müllerian and adult oviducts were dissected in PBS and fixed in 4% w/v PFA/PBS (Polysciences) for 30 minutes at room temperature followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... At 18 DIV Cells were fixed using 4 % PFA for 15 min and mounted on glass slides using Aqua-Poly/Mount (Polysciences Inc., Eppelheim). The experimenter was blinded to all the conditions ...
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bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Cell Biology 2023Quote: For Standard Transmission Electron Microscopy (TEM) a 4-5 mm long roots were fixed in 3% glutaraldehyde (Polysciences) in PBS pH 7.2 overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Immunology 2021Quote: ... the fly abdomens were opened in PBS and subsequently fixed for 20 min with 4% PFA in PBS (Polysciences). Aqua Polymount (Polysciences ...
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bioRxiv - Microbiology 2020Quote: ... infected cells then were fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... A minimum of 4 weeks after viral injections fluorophore-conjugated retrograde tracers (Dextran 10,000MW, AlexaFluor 488 and 568, Invitrogen; FastBlue, Polysciences) were injected bilaterally into Area X ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Grids were stained in uranyl-oxalate at pH 7 and contrasted in a mixture of 4% uranyl acetate (Polysciences) and 2 % methyl cellulose (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: Guts from adult female flies were dissected in PBS and immediately fixed in 4% formaldehyde (FA, EM grade, Polysciences) diluted in PBS for 1h at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...