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Citations for Polysciences :
151 - 200 of 210 citations for 3 3 Dimethyl 6 oxa 3 phosphoniabicyclo 3.1.0 hexane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... four L square glass bottles each containing 200 mL of suspension culture at ∼2.5 × 106 cell/mL were transfected using 1 µg/mL DNA and 3 µg/mL polyethylene ‘Max’ 40 kDa (Polysciences, Warrington, PA, #24765). The pre-premixing of DNA and PEI-Max was incubated for 20 min at room temperature before added to the bottles ...
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bioRxiv - Immunology 2023Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) were incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were then washed for 10 minutes in PBS (3 times) and stained with a 1:1 mixture of 2% aqueous osmium tetroxide (cat# 23310-10, Polysciences, Inc., Warrington, PA, USA) and 5% potassium dichromate for 48 hours ...
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bioRxiv - Immunology 2024Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) and incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Neuroscience 2024Quote: Under isoflurane anaesthesia (1.5-2%) a small incision was made at the Achilles tendon and the tracer Fast Blue (FB, Polysciences, 1.5% in 3 µL PBS) was injected into the soleus muscle and Alexa Fluor 488-conjugated Cholera Toxin subunit B (CTB 488 ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected at a cell density of 2.5×106 cells/mL by co-transfecting plasmids (1.1 mg total DNA/L cells) using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells).
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Neuroscience 2024Quote: ... with rat GRIK1-1aEMplasmid [or baculovirus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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bioRxiv - Immunology 2023Quote: Polybead amino 6 µm-diameter microspheres (Polysciences) were prepared to covalently couple rCD6 or rICAM1 ...
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bioRxiv - Cell Biology 2021Quote: ... 200 μL of 6% paraformaldehyde (Polysciences 18814-10) in PBS was added to each well and incubated for 10 min ...
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bioRxiv - Biophysics 2020Quote: ... we dispensed 6 μm diameter polystyrene beads (Polybead microspheres, Polysciences) diluted in HBSS into recording μ-slide channels and let them settle to the bottom of the μ-slide ...
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bioRxiv - Biophysics 2023Quote: ... Latex microspheres of 6 µm diameter (Polysciences Inc., PA, USA) were used to determine the zero-pressure level ...
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bioRxiv - Cell Biology 2022Quote: ... After 6 h of LPS treatment 16% Formaldehyde (18814-20, Polysciences Inc.) was added to the medium in the dish to a concentration of 1% and incubated (gently mixing ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Immunology 2022Quote: ... and 6 µl of Fluoresbrite Polychromatic Red Microspheres (Polysciences Inc., Warrington, PA) and the mix was top-plated onto 6-cm NGM plates ...
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bioRxiv - Neuroscience 2023Quote: ... were vortexed with 4–6 µg polyethylenimine (PEI Max, Polysciences, Inc., Warrington, PA) and incubated for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µl of 6-µm-diameter polystyrene MB suspension (containing 6.3 × 106 beads, Polysciences), and 1 µl of PBS containing 30% Healon was injected into the anterior chamber using a 32-gauge needle (Hamilton Company ...
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bioRxiv - Genetics 2024Quote: ... 6-10 larvae were placed in molds (Peel-A-Way disposable embedding molds, Polysciences) and sucrose solution removed ...
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bioRxiv - Immunology 2021Quote: 2000 synchronized L1 worms were mixed with 6 μl fluorescent beads (Fluoresbrite Polychromatic Red Microspheres, Polysciences Inc.), 25 μl 10X concentrated OP50 E ...
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bioRxiv - Bioengineering 2021Quote: ... Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photoinitiator (6 v/v %) and methacrylated rhodamine (2.5 v/v %) (Polysciences, Inc.) were added to the solution to facilitate photoinitiated crosslinking and fluorescent visualization ...
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bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
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bioRxiv - Immunology 2021Quote: 1200 synchronized L1 worms were mixed with 6 μl fluorescent beads (Fluoresbrite Polychromatic Red Microspheres, Polysciences Inc.). 25 μl 10X concentrated OP50-1 bacteria and M9 to bring the total volume to 300 ul ...
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bioRxiv - Immunology 2023Quote: 1,200 synchronized L1 worms were mixed with 6 μl fluorescent beads (Fluoresbrite Polychromatic Red Microspheres, Polysciences Inc.), 25 μl 10X concentrated OP50-1 E ...
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 100 mm diameter dishes to 90% confluency and were subsequently transfected with 6 μg of pCG1_SARS-2-S plasmid using polyethylenimine (PEI) Max (MW = 40,000KDa, Polysciences, Germany) as transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Immunology 2024Quote: ... 1,200 synchronized L1-stage worms were combined with 6 µl of fluorescent beads (Fluoresbrite Polychromatic Red Microspheres, Polysciences Inc.), 25 µl of a 10X concentrated OP50-1 E ...
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A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordatesbioRxiv - Developmental Biology 2024Quote: ... and empty vector was used to transfect each of 6 well plate(s) and 7.5 µL of linear polyethylenimine (PEI; Polysciences), MW 25,000 (1mg/mL ...
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bioRxiv - Zoology 2024Quote: ... Exsheathed larvae and adult worms were cultured in the presence/absence of 0.5 mm FITC labelled and/or 6 mm PE labelled fluorescent beads (Polysciences) for 5 days at 0.5µM (1 x107 beads/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and the slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Physiology 2020Quote: ... were transiently co-transfected with either A1R-Nluc or A3R-Nluc and β-arrestin2-YFP (total 2 μg, at a 1:6 ratio) using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Microbiology 2020Quote: 293T cells were grown to ~70% confluence and transfected with a mixture of 2 μg of DNA encoding the gRNA and 6 μL of Polyethylenimine (PEI, Polysciences catalogue #24765 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µg of DNA diluted in 100 µL of NaCl 150 mM was mixed with 6 µL of PEI Max 40000 (Polysciences) at 2 mg/mL diluted in 100 µL of NaCl 150 mM ...
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bioRxiv - Microbiology 2022Quote: ... and HNRNPUL1-V5 or MECR-V5 were forward transfected into 293T cells using PEI (PEI MAX, Polysciences; 6-well plates). Cells were lysed in co-IP buffer ...
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bioRxiv - Biophysics 2024Quote: ... Transfection solution was prepared by combining 6 µL (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 200 µL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were subsequently co-transfected with corresponding plasmid mixtures for 6 hours with 50 μL PEI (Catalog no. 24765, Polysciences; molecular weight 40,000 ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were transfected at 70% confluency in a 6-well plate or 12-well plate using PEI MAX® - Transfection Grade Linear Polyethyleneimine Hydrochloride (MW 40,000) (Polysciences) at a 1:3 DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed with 1 μg of DNA per single well of a 6-well plate using PEI MAX (Polysciences, INC.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... one half of the ovaries of each of 6 crickets was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, USA) in 100 mM phosphate buffer ...
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bioRxiv - Bioengineering 2022Quote: ... and gRNA expression plasmid (6 μg) were co-transfected with transfection reagent at a 2.6/1 ratio of Polyethylenimine (PEI; Polysciences, Warrington, USA) (μg)/total DNA (μg) ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 and HEK293 β-arrestin1/2 KO cells were transfected in suspension with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 million cells ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 × 106 cells ...