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Citations for Polysciences :
151 - 200 of 267 citations for 2 Pyridylacetic d2 Acid OD DCl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 1 μg ml-1 DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences), pre-incubated for 15 min in Optimem medium (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of plasmids were pre-incubated with 2 mg of 40-kDa linear polyethylenimine (PEI) (Polysciences) in 25 ml of Opti-MEM™ (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg/mL of each expression vector using 2 μg/mL Polyethyleneimine “MAX” (PolySciences) (Hirayama et al. ...
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bioRxiv - Microbiology 2021Quote: ... 1 cm catheter with biofilm formed on inside surface was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... spores were harvested in KK2 buffer and fixed with 2% paraformaldehyde with 2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer (pH 7.2 ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Live animals were immobilized with 0.01% tetramisole and 0.1% tricaine in M9 buffer and mounted on 2% agarose pads or with 0.05 μm Polybead microspheres (Polysciences) diluted 1:2 in water and mounted on 5% agarose pads ...
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20U/ml) for 2 min at 35°C followed by 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% formaldehyde (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20 U/ml) for 2 min at 35°C followed by 0.15 M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% paraformaldehyde (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Immunology 2023Quote: ... 4µg pMDG.2 plasmid and 4µg psPAX plasmid by using the transfection reagent Polyethylenimine (PEI) (Polysciences #23966-100) at a PEI:DNA ratio of 4:1 ...
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bioRxiv - Synthetic Biology 2022Quote: Worms were immobilized by 0.5 M NaN3 treatment (Figs. 1 and 2) or polystyrene nanoparticles (2.5% by volume, 0.1 μm diameter, Polysciences) (Figs ...
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bioRxiv - Molecular Biology 2022Quote: ... 293T media was changed and the cells were transfected using 1mg/ml of polyethylenimine (PEI) (Polysciences, 23966-2).
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bioRxiv - Immunology 2022Quote: ... HEK293E cells were transfected at a density of 106 cells/mL using 0.5 mg/mL of pTT3-RBD-HISAVI DNA and 2 mg/mL filter sterilized pH 7.0 Polyethylenimine (PEI) MAX (40,000 MW, Polysciences). Six days later ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by 2 minutes incubation of the slices in 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences). rinsed 5 × 2 min in chilled buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The dyes injected included fluorescent and other tracers: Fast Blue (FB, case AT, 3% dilution, 2 µl; Polysciences), Diamidino Yellow (DY ...
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bioRxiv - Cell Biology 2022Quote: ... Whole larva were then mounted between a 2% M9 agarose pad and coverslip in M9 containing 0.1μm polystyrene beads (Polysciences, Inc.) and 10 mM Levamisole to induce worm paralysis (Reich et al. ...
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bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...
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bioRxiv - Immunology 2020Quote: Mice were given a single oral gavage of 2 × 1011 of Fluoresbrite Yellow Green labelled 200 nm microbeads (Polysciences, Hirschberg an der Bergstrasse ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Biophysics 2022Quote: ... The cells in each well were transiently transfected with 1 μg HcKCR1/2-CGFP DNA plasmid using 3μg linear polyethylenimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and 2 μm-diameter fluorescent beads were added to each sample as an internal standard (Polysciences, Warminster, PA, USA). The samples were analyzed on a FACSCanto™ II Flow Cytometry Analyzer Systems (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 100 mm diameter dishes to 90% confluency and were subsequently transfected with 6 μg of pCG1_SARS-2-S plasmid using polyethylenimine (PEI) Max (MW = 40,000KDa, Polysciences, Germany) as transfection reagent ...
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bioRxiv - Immunology 2023Quote: ... luciferase encoding reporter plasmid (pTG126) and plasmid encoding Wuhan strain SARS-CoV-2 S using 24 μL polyethylenimine (Polysciences) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using PolyJet™ DNA In Vitro Transfection Reagent (Signagen, #SL100688) or PEI (Polysciences Inc, 23966-2) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: ... PRMT6-HA or Pu.1 as indicated using Polyethylenimine according to the manufacturer’s protocol (PEI, Polysciences, Cat#24765-2)
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bioRxiv - Microbiology 2022Quote: Leishmania promastigotes were harvested in logarithmic growth phase and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM phosphate buffer ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Molecular Biology 2024Quote: The pcDNA3.4 plasmid containing His-tagged SARS-CoV-2 spike protein was transfected to Expi293T cells using PEI max (Polysciences). After 18–21 h post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated for 24 hours at 37°C before the cells were transfected with 2 µg cDNA coding for hASIC1a (inserted into pcDNA3.1+ vector upstream IRES GFP) using Polyethylenimine (PEI) 25K (Polysciences) in a 1:3 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK 293T cells were transfected with 8 µg expression vectors and 2 µg of third-generation lentiviral packaging system in complex with 24µg PEI (Polysciences) in 1.2 ml of 0.9% NaCl ...
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bioRxiv - Cell Biology 2024Quote: Worms were anesthetized in 0.1 mg/ml tetramisole in 1X M9 buffer on empty NGM plates and mounted on thin 2% agarose pads on glass slides with 0.05 mm Polybead microspheres (Polysciences) for immobilization ...
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bioRxiv - Cell Biology 2020Quote: ... MLV-GagPol/pHIV 8.1 and pHIT VSVg at a ratio of 2:3:1 for 48 h using polyethylenimine (Polysciences, Germany). 293T supernatant was collected and filtered through a 0.2-μm filter before being used to transduce MDCK II cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cutaneous afferents innervating both hind paws of male C57BL/6J mice were labelled with the retrograde tracer Fast Blue (2% w/v in sterile PBS; Polysciences) when animals were aged 9 weeks ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...