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Citations for Polysciences :
151 - 200 of 1114 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... were stained with 2% uranyl acetate (Polysciences; Cat#6159-44-0) and lead citrate (Polysciences ...
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bioRxiv - Cancer Biology 2022Quote: ... and pLP/VSVG (2 µg) (clontech) using Polyethylenimine (PEI) Max (Polysciences). Virus containing supernatant was collected 48 h after transfection and cleared through a 0.45-µm PDF filter ...
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bioRxiv - Neuroscience 2020Quote: ... and 48 μl of 2 mg/mL polyethyleneimine (PEI; Polysciences, 24765) were mixed and transfected into Lenti-X 293T (Clontech ...
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bioRxiv - Physiology 2021Quote: ... Cells were transfected with Polyethylenimine-PEI Transfection Reagent (Polysciences, 23966-2) carrying the LC3-eGFP plasmid (60 ...
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bioRxiv - Cell Biology 2023Quote: NGN2-iN lentiviruses were produced by polyethylenimine (Polysciences, PEI, 23966-2,) in HEK 293T cells (ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... using Polyethylenimine according to the manufacturer’s instructions (PEI, Polysciences #24765-2).
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bioRxiv - Cancer Biology 2023Quote: ... were transfected into HEK293FT cells using 2 μg of Polyethylenimine (Polysciences). Lentiviral particle containing media was filtered using 0.45μm cellulose acetate syringe filter and used for transduction ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfection of packaging cells was performed using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... with 2 μg Polyethylenimine in water (PEI, pH 7.3, 23966, Polysciences), 50 ng plasmid DNA and 150 ng mock-DNA (empty plasmid) ...
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bioRxiv - Cell Biology 2024Quote: ... with 2 µg Polyethylenimine in water (PEI, pH 7.3, 23966, Polysciences), 50 ng plasmid DNA and 150 ng mock-DNA (empty plasmid) ...
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... and 2,4,6-Tris-(dimethylaminomethyl)phenol (DMP- 30, Polysciences, Inc., cat# 00553–100). On the next day ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3μl of 1.25% (w/v) polystyrene microspheres (Polybead Microspheres 0.05μm, Polysciences) and 0.2mM levamisole (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently transferred to 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... were produced as previously described using a PEI (polyethylenimine, Polysciences, 24765-2) transfection protocol followed by iodixanol gradient purification ...
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bioRxiv - Neuroscience 2023Quote: ... with 2 μl of 100 nm polystyrene beads (Cat# 00876-15, Polysciences) and 2 μl of levamisole (from 500 μM stock ...
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bioRxiv - Cell Biology 2022Quote: ... and 48 μL of Polyethylenimine ‘Max’ MW 40,000 Da (Polysciences; 24765-2) per plate ...
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bioRxiv - Pathology 2024Quote: ... Vector preparations were generated by polyethylenimine or PEI (Polysciences, Cat #24765-2) triple transfection of pACM-Delta ITR-flanked transgene ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS or HeLa cells were transfected using polyethylenimine (PEI) (23966-2 Polysciences) at a 1:3 plasmid to PEI ratio ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Immunology 2024Quote: HEK-293T cells were co-transfected with Env-expressing psvIII plasmid and a HIV Env deficient backbone plasmid (pSG3△Env) at a mass ratio of 1:2 using Polyethylenimine (PEI) max (Polysciences, Bergstrasse, Germany) at a DNA:PEI mass ration of 1:3 and incubated for 48 hours at 37°C 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... with modified pCW57.1 target vector in a ratio of 5 : 1 : 5 : 5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966). Three days post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with PEI (Polyethylenimine 25kD linear from Polysciences, cat# 23966-2). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection was performed using polyethylenimine (#23966-2; Polysciences Inc., Warrington, PA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... All transfections were carried out using linear polyethylenimine (PEI; Polysciences cat. #23966-2) by standard protocols.
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bioRxiv - Microbiology 2020Quote: ... All samples included 2 μm diameter Fluoresbrite beads (Polysciences Inc., Warrington, PA, USA) for size reference and alignment purposes ...
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bioRxiv - Cell Biology 2023Quote: ... and psPAX.2 (3.75 μg) with PEI MAX 40K (Polysciences Inc., # 24765-100) (1:3 DNA to PEI ratio) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... HEK cells were transfected using Polyethylenimine (PEI) (1mg/mL) (Polysciences, cat# 23966-2). 3µg plasmid DNA (6-well dishes ...
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bioRxiv - Genomics 2022Quote: ... the samples were contrasted by 2% uranylacetate (Polysciences Inc, Cat No 21447-25) in water for 1 minute ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were fixed with 2% osmium tetroxide in PBS (Polysciences, 23310-10) for membrane preservation ...
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bioRxiv - Cancer Biology 2024Quote: Transfections in HEK293T cells were performed using polyethylenimine (PEI; Polysciences Inc #23966-2) following standard protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% formalin (Polysciences, 04018-1), permeabilized with 0.3% Triton X-100 in 1X PBS for 20 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... The 4 plasmids were incubated for 15 minutes with 5 μL of polyethylenimine (PEI, Polysciences #23966) before transfection ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...