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Citations for Polysciences :
151 - 200 of 658 citations for 2' 5' Dichloro 3 3 4 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg): PEI (μL) (Polysciences, USA) at 1mg/mL in molecular grade water ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Immunology 2023Quote: ... 0.5 mM spermidine in 1X protease inhibitor buffer) and incubated with activated concanavalin A (ConA) coated magnetic beads (Polysciences - 86057-3) in PCR strip tubes at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... to induce LD formation and then cells were transfected with 3 µg of plasmid DNA/mL using Polyethylenimine HCl MAX (Polysciences, Inc) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... 1.1 mg of a 1:1 (w/w) mixture of the GABAAR α1 subunit and NACHO DNA and 3 mg of Polyethylenimine “Max” (Polysciences #24765) were dissolved separately in 30 mL Expi293 expression media ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
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bioRxiv - Physiology 2024Quote: ... The balloon was inflated after the first diagonal branch of the LAD in each animal and 3 ml polystyrene microspheres (Polybead, 90 μm, Polysciences, Warrington, PA) were slowly injected via the lumen of the angioplasty catheter ...
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bioRxiv - Neuroscience 2024Quote: ... Food was prepared by resuspending 1 g of sera micron (Sera 00720) in 45 mL of 1/3 MR with 30 µL of 6 µm fluorescent bead solution (Polysciences 18862-1) added ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1 µg per one million cells of the plasmids containing CMV promoter and 3×FLAG-tagged geneswere mixed with a three-fold excess (w/w) of polyethylenimine MAX 40 kDa (Polysciences, Warrington, PA, USA) in 4 ml OptiPro (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were incubated for 3 h at RT in the mixture of EtOH and LR White resin (Polysciences, Inc., Warrington, PA, USA) in the following proportions ...
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bioRxiv - Microbiology 2022Quote: ... followed by a 1:2 mixture of propylene oxide for and Polybed 812 epoxy resin for 3 hours and transferred to 100% resin to embed overnight (Polysciences, Inc.,Warrington, PA). Ultrathin sections (70-80 nm ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Synthetic Biology 2022Quote: Worms were immobilized by 0.5 M NaN3 treatment (Figs. 1 and 2) or polystyrene nanoparticles (2.5% by volume, 0.1 μm diameter, Polysciences) (Figs ...
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bioRxiv - Immunology 2023Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) were incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Immunology 2024Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) and incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected at a cell density of 2.5×106 cells/mL by co-transfecting plasmids (1.1 mg total DNA/L cells) using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells).
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Neuroscience 2024Quote: ... with rat GRIK1-1aEMplasmid [or baculovirus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... retinas were washed 4 times with PBS for 5 minutes prior to whole mounting onto slides with fluorescent mounting medium (Polysciences, Cat#18606-20). Slides were allowed to dry prior to imaging ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The decalcified tibiae were immersed in a 1:1 mixture of 5% potassium dichromate and 2% osmium tetroxide (23310-10, Polysciences, Inc., USA) for 48 h and stained ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transporter 5™ reagent (Polysciences, Inc, Cat #26008-5) was used to transfect parental HEK293T/17 cells ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Neuroscience 2024Quote: ... They were fixed overnight at 4°C in 4% paraformaldehyde (PFA) (Polysciences) diluted in 1X Phosphate Buffered Saline ...
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bioRxiv - Biochemistry 2023Quote: ... -MCM9 M45E or -GFP control (Transporter 5, Polysciences 26008-5) and harvested 3 days later for HA-immunoprecipitation as previously described39 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Molecular Biology 2024Quote: ... with modified pCW57.1 target vector in a ratio of 5 : 1 : 5 : 5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966). Three days post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Fast Blue (5%, Polysciences) or rAAV-hSyn-EGFP (1012vp/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 2 ml 2% PFA (Polysciences, Inc., 18814). After 30 min incubation at room temperature (RT) ...