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Citations for Polysciences :
101 - 144 of 144 citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Samples from NHP were depleted using purified DENV or BSA control protein adsorbed onto Polybeads polystyrene microspheres (Polysciences, Inc.) as reported earlier (11 ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Biochemistry 2019Quote: ... followed by ∼3 microliters of the final assay solution containing 5 ng mL-1 500 nm diameter beads (PolySciences Inc,), coated nonspecifically with neutravidin18(ThermoFisher Inc.).
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... transient transfections of 5 µg of pcDNA3.1-based vectors were performed using 25 µg of PEI MAX (Polysciences, Warrington, PA). After an additional incubation for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the pNL4-3-inGluc and S constructs in a 2:1 ratio using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) in order to generate viral particles ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly seeded 293T cells grown in DMEM supplemented with 10% FBS + 50 µg/mL gentamycin at 37°C with 5% CO2 (hereafter, supplemented DMEM) were transfected using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 8 µg of plasmid DNAs (pCMV-Thy1.1-F2A-dsGFP/muSOX/SOX/vhs/BGLF5) using 48 μl Transporter 5 (Polysciences #26008) and 1ml Opti-MemI (Gibco #31985070) ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Slit2-Fc (N-(aa1-840) and C-terminal (aa841-1290) fragments) proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were produced by transient transfection of plasmids into HEK 293F cells using the polyethylenimine (PEI) Max reagent (Polysciences, Inc.) with a 1:3 ratio (wt/wt ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Neuroscience 2021Quote: ... The surface of the substrate was plasma treated at 500 W for 5 min and coated with an aqueous solution of 10% (wt/vol) Poly(acrylic acid) (MW 50000, Polysciences, USA) at 2000 rpm for 15 s using a spin-coater (WS-650-23 ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Neuroscience 2022Quote: ... vectors were transfected to 50-80% confluent HEK293T cells in 35-mm dish using PEI-MAX (25 mM, 5 μL/dish; Polysciences, Inc.). Twenty-four hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Control and 5-FU-miR-129 groups were treated with either 75 μg of PEI-MAX (control) (Polysciences, Warrington, PA, USA) or 75 μg PEI-MAX + 75 μg 5-FU-miR-129 (5-FU-miR-129) ...
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bioRxiv - Neuroscience 2022Quote: ... was injected into the gastrocnemius muscles and the tracer Fast Blue tracer (Polysciences cat.no. 17740-5 at 1.5% in 3μL PBS) was injected into the soleus muscles ...
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bioRxiv - Synthetic Biology 2023Quote: ... they were transfected by mixing roughly 600 ng of plasmid mixture per well with 1.6 μL Transporter 5 Transfection Reagent (Polysciences, Warrington, PA) in 60 μL 150 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... and NiV-M-GFP at a 9:1:5 ratio by using polyethylenimine (PEI) at 1 mg/ml (Polysciences, 23966-100). At 48hrs posttransfection ...
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bioRxiv - Biophysics 2021Quote: ... in a microcentrifuge tube and resuspending in coupling buffer (400 μl in first wash and 170 μl in second washing) (PolyLink Protein Coupling Kit, Polysciences Inc.). Then 20 μl of the freshly prepared EDCA solution (20 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Pathology 2019Quote: ... Sections were washed with PBS/5% goat serum 3 times for 3 minutes each and fixed with Aqua-Mount (Polysciences, Warrington, PA). Control kidney sections lacked primary antibodies.
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bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
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bioRxiv - Molecular Biology 2021Quote: 100 million cells were seeded in 15 cm dishes and the protein-DNA contacts were cross-linked using 1% (v/v) formaldehyde (Polysciences, 04018-1) for 10 min at room temperature with shaking ...
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bioRxiv - Immunology 2020Quote: ... BioInvent n-CoDeR® scFv were selected using biotinylated in-house produced recombinant LILRB3-hFc recombinant fusion proteins (captured with streptavidin-coated Dynabeads®) with or without competition or LILRB1-hFc coated to etched polystyrene balls (Polysciences, US) or plastic immunotubes ...
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bioRxiv - Cell Biology 2020Quote: ... The TEM samples were prepared by dropping 5 μl of solution onto a 400-mesh carbon film-coated copper grids (24945-50, Polysciences, Inc., PA, USA), which were glow-discharged by a plasma cleaner ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Biophysics 2023Quote: ... A volume of 20 μL of 25 mM levamisole mixed with 5 μL 200-nm-sized polystyrene beads (Polysciences Inc., Warrington, PA, USA) was dropped onto the cover glass ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cell Biology 2019Quote: ... 1.5 × 108 cells expressing GFP-Nup131 were fixed in 1 mL of a mixture of 4% formaldehyde (18814-10, Polysciences, Inc, Warrington, PA, USA) and 0.01% glutaraldehyde (1909-10 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...