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Citations for Polysciences :
101 - 150 of 1050 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cells in a single well of 6-well plate (confluency ≥80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1% glutaraldehyde (Polysciences) in 0.1 M cacodylate buffer (Polysciences ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... with 1 mg of total DNA and 4 mg of PEI (Polysciences, 24765) per 1 liter of cells at a density of 1.5-2 x 106 cells/ml ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences, #23966-1) diluted in 18.8 mL Opti-MEM® ...
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bioRxiv - Immunology 2022Quote: ... 1 mg of plasmid was mixed with 4 mg of polyethyleneimine (PEI; PolySciences Inc.) in Opti-MEM cell culture medium (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 60mL ice-cold 4% paraformaldehyde (PFA) (ref # 00380-1, Polysciences, Warrington, PA) in 0.1 M phosphate buffer (80 mM Na2HPO4 and 19 mM NaH2PO4 ...
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bioRxiv - Physiology 2024Quote: ... Hearts were then embedded using a JB-4 kit (Catalogue number 00226-1, Polysciences). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Immunology 2024Quote: HEK-293T cells were co-transfected with Env-expressing psvIII plasmid and a HIV Env deficient backbone plasmid (pSG3△Env) at a mass ratio of 1:2 using Polyethylenimine (PEI) max (Polysciences, Bergstrasse, Germany) at a DNA:PEI mass ration of 1:3 and incubated for 48 hours at 37°C 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... into one 15-cm plate of confluent HEK-293T cells using PEI Max (Polysciences Inc., catalog # 24765-1). At one day post-transfection we changed the medium to low serum (1% FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 1% tannic acid and 1% uranyl acetate (Polysciences Inc. ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Molecular Biology 2024Quote: ... before the ROS generating agent 2,2’-Azobis(2-amidinopropane) dihydrochloride (AAPH; final conc. 1 mM in media; 08963, Polysciences, Inc., Warrington, PA, USA) was added for over-night incubation ...