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Citations for Polysciences :
51 - 100 of 596 citations for Nectin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Developmental Biology 2019Quote: Fluorescent red beads of 1 uM diameter (1:500, 18660-5, Polysciences) were injected into the KV of embryos at the 6-somite stage ...
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bioRxiv - Microbiology 2019Quote: ... followed by 1 h of fixation in 1% osmium tetroxide (Polysciences Inc.). After extensive wash with dH20 ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 1% tannic acid and 1% uranyl acetate (Polysciences Inc. ...
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bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... and then mixed 1:1 with 0.2 M sodium carbonate–bicarbonate buffer (24095, Polysciences) containing 1 M sodium chloride (S671-3 ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent beads (1 μm, Polysciences) were added for internal calibration (105 mL−1) ...
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bioRxiv - Neuroscience 2020Quote: Fast Blue (Polysciences, 17740-1), CM-DiI (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... postfixed in 1% OsO4 (Polysciences) in 0.1M cacodylate buffer for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Polyethylenimine “Max” (24765-1, Polysciences) was used for transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Polyethyenimine (PEI) (Polysciences, 23966-1) was used as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... 1ml Polyethylenimine (23966-1, Polysciences), pLV delta 8.9 (16.5μg) ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Microbiology 2023Quote: ... then infiltrated in 1:1 mixture of propylene oxide:Polybed 812 epoxy resin (Polysciences, Warrington, PA) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized using isoflurane and 1-2μl of FB (1% in saline; Polysciences Inc.) was injected subcutaneously using a Hamilton syringe connected to a 30-gauge needle to label sensory afferents that innervate the injection sites ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was mixed 1:3 (w/w) with PEI 25 K (Polysciences, 23966-1) for 20 min at room temperature and then used to transfect HEK293S GnTi- cells at 37°C in 5% CO2 (ATCC ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then mounted in a 1:1 mixture of Aqua-Poly/Mount (Polysciences, 18606) and RapiClear (SUNJin Lab ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL PEI (Polysciences, #23966-1) in 50 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... or PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Microbiology 2020Quote: ... or PEI MAX40 (Polysciences 24765-1) following the manufacturers’ recommendation.
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bioRxiv - Developmental Biology 2022Quote: ... with PEI reagent (Polysciences, 23966-1). Lentiviral particles were collected 48 h and 72 h after transfection and filtered.
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bioRxiv - Genetics 2021Quote: ... or PEI MAX (Polysciences, 24765-1) according to manufacturer instructions.
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bioRxiv - Bioengineering 2022Quote: 1 µm yellow-green beads (Polysciences) were flowed through a capillary tube by pulling at 250 nL/min (rectangular capillary ...
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bioRxiv - Microbiology 2022Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer at 4ºC for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer for 30 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... PEI at 1 mg/mL (Polysciences) was used as the transfection agent ...
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bioRxiv - Bioengineering 2021Quote: ... 1 uL (or 1.5 uL for target-binding experiments) of 1 mg/mL PEI (Polysciences, 24765-2) dissolved in water was added to DNA in media and vortexed for 10 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...