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Citations for Polysciences :
51 - 100 of 179 citations for N BOC 3 FLUORO L TYROSINE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... All protein encoding sequences were subcloned to pCAG vector with N-terminal FLAG-tag for expression in HEK293F cell lines transfected by polyethylenimines (PEIs, Polysciences). Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Genomics 2024Quote: ... CaGHV-1 RTA was expressed as an N-terminally 3xFLAG-tagged protein in HEK293T cells by transfecting the cells with PEI (Polysciences). The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg polyethylenimines (PEIs, Polysciences) in 50 mL SMM 293T-II medium (Sino Biological Inc. ...
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bioRxiv - Cell Biology 2021Quote: Latex NH2-beads (3 μm diameter, 3.5 x 108 beads/preparation, Polysciences) were activated with 8% glutaraldehyde in 0.5 ml PBS for 120 min under rotation at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: Aliquot 15 μL of Concanavalin A (ConA) beads (Polysciences cat#86057-3) and bind to magnet for 5 minutes ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... or PEI Max (3 µg PEI per 1 µg DNA) (Polysciences 24765) (for substrate only transfection for investigating endogenous PS (Fig 5 & 6) ...
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bioRxiv - Immunology 2023Quote: ... 3 μl of linear PEI (1mg/ml, Cat. No. 23966-1, Polysciences) transfection reagent was used per 1 μg of plasmid DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... BioMag®Plus Concanavalin A-coated magnetic beads (#86057-3, Polysciences, USA), previously activated with 20 mM HEPES pH 7.9 ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2.0×106 cells/ml and co-transfected with pMlink-N-FLAG-p22phox-NOX2 and pMlink-EROS using PEI 4000 reagent (Polysciences, Warrington, PA). The transfected cells were cultured for 48 h and collected by centrifuged (4,000 × g ...
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bioRxiv - Biochemistry 2024Quote: ... was expressed with a TEV protease cleavable N-terminal 6xHis-tag and IgK secretion signal in HEK293 GnTI-cells upon transient transfection with PEI MAX (Polysciences Inc., 24765). The IgK secretion signal allowed for the secretion of LyLAP recombinant protein ...
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bioRxiv - Developmental Biology 2021Quote: ... After washing 3 times in PBS sections were mounted with Aquapolymount (Polysciences Inc.), and coverslipped (Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... bEnd.3 cells were fixed in 4% cold PFA (Polysciences, Inc. PA, USA), and the immunofluorescence staining was performed following the same procedures as stated above.
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with plasmids of interest (Table 3) using polyethylenimine (PEI, Polysciences). At 4 h post-transfection ...
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bioRxiv - Bioengineering 2020Quote: ... templating rods were placed and porous sponges composed of 50% poly-L-lactic acid (PLLA) (Polysciences, Warrington) and 50% poly(lactic-co-glycolic acid (PLGA ...
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bioRxiv - Synthetic Biology 2024Quote: Transient transfection was performed using polyethylenimine (PEI) (Polysciences, 23966, prepared as a .323 g l-1 stock). For each transfected well of a 24-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... rinsed in PBS for 3 h and embedded in polyethylene glycol (MW = 1500; Polysciences). Six-ųm sagittal sections were cut on a rotary microtome ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of plasmid was mixed with 3 μg of polyethylenimine (MW 25,000; Polysciences) or Expifectamine (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2021Quote: ... 500,000 OSCs were bound to 10 µl Concanavalin A-coated magnetic beads (Polysciences, #86057-3) and lysed using Dig-wash buffer (20 mM HEPES pH 7.3 ...
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bioRxiv - Cell Biology 2021Quote: ... about 1.5 mg of the plasmid was premixed with 3 mg of polyethylenimines (PEIs) (Polysciences) in 50 mL of fresh medium for 15 mins before adding to cell culture ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 3 time in 1xPBS before being mounted with Aqua Polymount (Polysciences, 18606-20). The following primary antibodies were used ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Developmental Biology 2023Quote: ... Strips were washed 3 times with PBS and mounted on slides using Aquamount (18606, Polysciences) with the flat side of the strips facing the coverslip ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was mixed 1:3 (w/w) with PEI 25 K (Polysciences, 23966-1) for 20 min at room temperature and then used to transfect HEK293S GnTi- cells at 37°C in 5% CO2 (ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... packaging vectors at a ratio of 4:3:1 using polyethyleneimine (PEI, Polysciences, #23966-2). Virus-containing medium was collected at 48 and 72 hours post-transfection ...
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bioRxiv - Genetics 2022Quote: ... pCS2-3xFlag-SLP-L or pCS2-3xFlag-SLP-S was transfected into HEK293T cells by polyethylenimine (PEI) MAX (Polysciences, 24765). Cells were harvested 24-hour post-transfection and lysed by RIPA cell lysis buffer (Beyotime ...
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bioRxiv - Genomics 2023Quote: ... Expi293F cells at a density of 3×106 cells/mL were transfected with 1mg/L of culture of plasmid complexed with PEI Max 40K (cat. 24765-100, Polysciences) in a 1:2 plasmid:PEI w/w ratio in 40mL Opti-MEM (cat ...
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bioRxiv - Cancer Biology 2023Quote: Transient transfection for fluorescence microscopy was performed directly on coverslips coated with poly-L-lysine (see above) placed in 24-well plates using the transfection reagent Polyethylenimine (PEI; Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transiently transfected at a density of 3 × 106 cells/mL using linear polyethylenimine (Polysciences). 24 h following transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Cell Biology 2020Quote: ... MO)(Escherichia coli (0111:B4) and coated onto 3-micron polystyrene beads (Polysciences Inc., Warrington, PA) as previously described (Mantegazza & Marks ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... and LPS (100 μg/ml) coupled to 3-μm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mM spermidine and 1× protease inhibitor) with activated Concanavalin A Magnetic Beads (Polysciences, 86057-3) for 10 mins at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... at a 2:1:1 ratio using 3 μg 25 kDa linear polyethylenimine (PEI) (Polysciences Inc.) per μg DNA ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 minutes and mounted using AquaPolymount (Polysciences, Warrington, USA). Images were obtained on a Confocal and STED FACILITY line microscope (Abberior Instruments ...
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bioRxiv - Biochemistry 2020Quote: Plasmids (500 ng DNA per ml culture) and polyethylenimine (MW 25,000; Polysciences; 3 µg per ml culture) were mixed with OptiMEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Neuroscience 2020Quote: ... sections were subsequently washed with PBS 1X (3 × 10 min) and mounted in Aqua/polymount (Polysciences 18606).
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...