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Citations for Polysciences :
51 - 100 of 136 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... incubated with DAPI for 20 min and washed again before being mounted using Aqua-Poly/Mount (Polysciences). Sections were stored at 4°C until imaging ...
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Developmental effects of oxytocin neurons on social affiliation and processing of social informationbioRxiv - Neuroscience 2021Quote: ... mounted dorsally on a slide in mounting medium (Aqua-Polymount, Polysciences Inc., Warrington PA, CAT# 18606-20) and imaged by a Zeiss LSM 800 scanning confocal microscope.
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bioRxiv - Immunology 2022Quote: ... and mixed with 20 μl IMDM medium containing 1.2 μl of 1 mg/mL polyethylenimine (PEI, Polysciences). Following a 20 min incubation step at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids were diluted in DMEM and incubated for 20 min at RT with Polyethylenimine (PEI, 23966, Polysciences) at a ratio 1:3 DNA:PEI ...
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bioRxiv - Developmental Biology 2020Quote: ... For imaging and quantification immunohistochemistry brain slices mounted in Aqua-Poly/Mount (Cat No. 18606-20, Polysciences, Inc) with #1.5 thickness coverslips were imaged with a laser scanning confocal microscope Leica TCS SP5 ...
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bioRxiv - Cell Biology 2021Quote: ... counterstained in Mayers Hematoxylin solution (Fluka) and mounted under coverslips in Aqua Poly/Mount (18606-20, Polysciences, Inc).
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bioRxiv - Biophysics 2021Quote: ... Transfection solution was prepared by combining 20 μL (per dish, hereafter) of polyethylenimine solution (Polysciences; 1 mg/mL) and a plasmid mixture consisting of 500 ng Lg-βarr1 or Lg-βarr2 and 1μg μOR-Sm in 1 mL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Embryos were transferred with a glass needle and mounted in ~1.5μL of Boyd buffer containing ~50 polystyrene-based microspheres (20 μm in diameter, Polysciences) between a 24 × 50 mm glass slide and an 18 × 18 mm glass coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times with DPBS before embedding with Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) and a cover glass ...
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bioRxiv - Immunology 2021Quote: ... the fly abdomens were opened in PBS and subsequently fixed for 20 min with 4% PFA in PBS (Polysciences). Aqua Polymount (Polysciences ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were rewashed with 1x PBS and milli-Q water and finally mounted with AquaMount (Polysciences, 18606-20). To assess cell death ...
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bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed with 250 μL OptiMEM containing 20 μg of polyethylenimine (PEI – maximum molecular mass of 40,000 Da; Polysciences). This mixture was added to 20.0 × 106 Expi293F GnTI-Cells (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Cell Biology 2022Quote: ... animals were soaked in ScaleA2 (4M urea, 20% glycerol, 0.1% Triton X-100, 2.5% DABCO)38 and mounted in Aqua-Polymount (Polysciences Inc. 18606). Some in situ hybridizations were carried out in a CEM InSituPro Hybridization robot up to the development stage ...
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bioRxiv - Bioengineering 2022Quote: ... Methacrylation (20% theoretical) was performed to obtain OMA (5OX30MA) by reacting oxidized alginate with 2-aminoethyl methacrylate hydrochloride (AEMA, Polysciences). To synthesize OMA ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were dissected in 8-10 μL of Shelton’s Growth Medium (Edgar and Goldstein, 2012) and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip ...
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bioRxiv - Cell Biology 2023Quote: ... 4.5μg of lentiviral donor plasmid was added to the mixture and incubated for 20 min with 10μL of polyethylenimine (PEI, Polysciences 23966) dissolved in OptiMEM (Gibco 31985062) ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted embryos were transferred via a hand drawn glass capillary to a 2 uL drop of M9 buffer containing ∽100 polystyrene beads 20 um in diameter (Polysciences Inc.), sandwiched between two pieces of #1.5 coverglass ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
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bioRxiv - Neuroscience 2019Quote: ... slices were floated onto Superfrost™ glass slides with the membrane confetti in contact with the slide and Menzel-Gläser #1.5 glass coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Neuroscience 2019Quote: ... slides were dipped in distilled water and Menzel-Gläser #1,5 coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
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bioRxiv - Neuroscience 2019Quote: ... slices were floated onto Superfrost glass slides with the membrane confetti in contact with the slide and Menzel-Gläser #1,5 glass coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
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bioRxiv - Cell Biology 2019Quote: The tail fin was amputated up to the level of the notochord and larvae were fixed 20-24 hours later in 1ml of 4% ethanol-free formaldehyde (Polysciences, Warrington, PA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: Midguts were dissected in 1X PBS (Oxoid, BR0014G) and fixed for 20 minutes at room temperature in fresh 4% methanol-free paraformaldehyde (Polysciences, 18814-10) diluted in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 min and coverslips were mounted onto SuperFrost® Plus slides (R. Langenbrink) with fluorescent Aqua-Poly/Mount mounting medium (Polysciences, 18606-20). The cells were imaged using a Leica TCS SP5 confocal laser scanning microscope and an HCX PL APO lambda blue 63× oil UV objective or HCX PL APO 40.0×1.30 OIL objective controlled by the LAS AF scan software (Leica Microsystem ...
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bioRxiv - Biophysics 2023Quote: ... A volume of 20 μL of 25 mM levamisole mixed with 5 μL 200-nm-sized polystyrene beads (Polysciences Inc., Warrington, PA, USA) was dropped onto the cover glass ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Developmental Biology 2020Quote: Third instar larvae and adult CNS were dissected in 1x PBS and tissue was fixed for 20 minutes in 4% formaldehyde (Polysciences, Inc., 10% methanol-free) diluted in PBST (0.3% Triton X-100 in PBS) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: ... the PolyLink Protein Coupling Kit (Polysciences) was used as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: PolyLink Protein Coupling Kit (Polysciences, Inc) was used to prepare 50µl of fluorescence beads solution ...
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bioRxiv - Cell Biology 2020Quote: Ten million mouse embryonic fibroblasts (ATCC) were transfected with 90uL of Polyethylenimine (PEI MAX #24765 Polysciences) diluted in 240uL NaCl 150mM and 15ug of the pX335-gRNA and pUC19-homology arms-mCherry plasmids also diluted in 240uL NaCl 150mM ...
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bioRxiv - Biophysics 2019Quote: ... we used a protein coupling kit (Polysciences) to coat micro-spheres with WGA14 ...
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bioRxiv - Cancer Biology 2022Quote: ... Picrosirius Red stain Kit (Polysciences Inc., PA, USA) was used with a slight modification of the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The Fungi-Fluor® Kit (Polysciences, PA, USA) was applied following the manufacturers protocol ...
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bioRxiv - Biophysics 2021Quote: ... via Carbodiimide reaction (PolyLink Protein Coupling Kit, Polysciences Inc.). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... and Picrosirius red staining kit (Polysciences, Inc., Warrington, PA) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μg of human PANX1 plasmid and 25 μg of mouse Src Y529F mutant plasmid was used) was mixed with 150ug of PEI 25K (Polysciences) in a and incubated at room temperature for 30 min ...
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bioRxiv - Genetics 2019Quote: ... Samples were embedded using the Poly/Bed 812 Kit (Polysciences). After pure fresh resin embedding and polymerization in a 65°C oven (TD-700 ...
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bioRxiv - Cancer Biology 2021Quote: ... we used Rapid PTAH Stain Kit (Polysciences, Warrington, PA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were stained with Weigert’s Hematoxylin Kit (Polysciences, Warrington, USA) for 8 min and washed for 10 min under running tap water ...