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Citations for Polysciences :
51 - 100 of 966 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 1 μg ml-1 DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences), pre-incubated for 15 min in Optimem medium (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... 1.1 mg of a 1:1 (w/w) mixture of the GABAAR α1 subunit and NACHO DNA and 3 mg of Polyethylenimine “Max” (Polysciences #24765) were dissolved separately in 30 mL Expi293 expression media ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of plasmid was incubated with 3 mg of Linear PEI 25K (Polysciences) in 100 mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Food was prepared by resuspending 1 g of sera micron (Sera 00720) in 45 mL of 1/3 MR with 30 µL of 6 µm fluorescent bead solution (Polysciences 18862-1) added ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent beads (Fluoresbrite, 2 mm diameter, Polysciences, #18338-5) were diluted 1:500 in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... we used FastBlue (5% in distilled water, 17740-1, Polysciences, Inc, Warrington, PA), fluorescent latex microspheres (red retrobeads IX or green retrobeads IX ...
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bioRxiv - Physiology 2020Quote: ... were transiently co-transfected with either A1R-Nluc or A3R-Nluc and β-arrestin2-YFP (total 2 μg, at a 1:6 ratio) using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... neurons were stained with 1 mL of 2% uranyl acetate (Polysciences #21447-25) in water for 30 min at room temperature and then briefly washed with 50% ethanol (Pharmco #111000200 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg/μL PEI (Polyethylenimine HCl MAX Linear MW 40000 (PolySciences, 24765–2)) and 1 μg/ μL DNA (3:1 ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of 1 mg/ml linear Polyethylenimine (PEI; Polysciences, Inc, 24765-2) and 1-2 µg of each plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected using polyethylenimine (PEI) (Polysciences, 23966-2, 1 mg/mL) and 3rd generation lentivirus packaging vectors (pMDLg ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μl of 1 mg/ml linear Polyethylenimine (PEI; Polysciences, Inc, 24765-2) and 1–2 μg of each plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg DNA/ml transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mM spermidine and 1× protease inhibitor) with activated Concanavalin A Magnetic Beads (Polysciences, 86057-3) for 10 mins at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells/mL culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa PEI (Polysciences, 24765-1) per mL culture volume ...
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bioRxiv - Neuroscience 2021Quote: ... or 5 µl of 2% Fast-Blue (Polysciences, Warrington, PA, USA). Tracer leakage was prevented by sealing the reservoir around the nerve with Vaseline (Vaselinum album ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: A stock solution of 1 mg / ml linear Polyethylenimine (PEI; Polysciences, Inc 24765-2) was prepared in a buffer containing 25 mM Hepes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were picked up onto 1×2 mm formvar coated copper slot grids (Polysciences), and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Cancer Biology 2024Quote: ... The transfection was performed accordingly: 2 μl polyethylenimine (PEI) 25K (1 mg/ml; Polysciences) was mixed with each μg of plasmid in Opti-MEM medium (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... C3H10T1/2 cells were transfected with Transporter 5® Transfection Reagent (Polysciences) following manufacture’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Third-generation VSV.G pseudotyped HIV-1 lentiviruses were produced by polyethylenimine (PEI, Polysciences #23966-2)-transfection of HEK293T cells and packaged with VSVG-coats using established methods ...
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bioRxiv - Neuroscience 2022Quote: ... 4.1 µg of VSVG and 110 µL of Polyethylenimine (1 µg/µL; Polysciences, 23966-2). This solution was incubated for 10 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... bacmid (1 µg/mL culture volume) was mixed with 2 µg PEI 25K (Polysciences, Inc.) per µg bacmid in 200 µL warm PBS and incubated at RT for 30 mins ...
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bioRxiv - Physiology 2023Quote: ... Sections were picked up on 1 x 2-mm formvar-coated copper slot grids (Polysciences) and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Physiology 2020Quote: ... animals were post-fixed with 1% aqueous solution of osmium tetroxide (Polysciences Europe GmbH 0972B-5) and 1.5% potassium ferrocyanide (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Live worms were mounted on 2% agarose dry pads with 2% (for young adults) or 5% (for L1 larvae) polystyrene beads (Polybeads, Polysciences) in M9 buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 µm diameter or Dynabeads T1: 1 µm diameter) at concentrations of 5×106/µl (high) or 5×105/µl (low) or polystyrene beads (Polysciences, 64090-15: 6 µm diameter) at concentrations of 1×105/µl (high ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 1 mg/mL plasmid DNA was mixed with 2 mg/mL linear 25 kDa polyethyleneimine (Polysciences) and transfected in OptiPRO SFM medium (4% final volume) ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Biophysics 2022Quote: ... The transfection solution was prepared by combining 5 μL of 1 mg/ml polyethylenimine Max solution (Polysciences) and a plasmid mixture consisting of 200 ng ssHA-FLAG-NTS1 and 500 ng alkaline phosphatase (AP)-tagged TGF-α (AP-TGFα ...
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bioRxiv - Biophysics 2020Quote: ... and GFP in 1:1:1 ratio using polyethyleneimine (54) (Polysciences, Inc.; 23966-1; lot: 705149). We verified each construct by sequencing before and after subcloning ...
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bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...