Labshake search
Citations for Polysciences :
801 - 850 of 975 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... samples were washed 3 times for 30 mins with PBS then incubated through a cryoprotective series of sucrose solutions of increasing concentration from 10-50% for ≥3 hours each before embedding into OCT compound (Tissue Tek) in disposable cryomolds (Polysciences) and frozen in an isopentane bath cooled by liquid nitrogen ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells grown in 10 ml of medium (3.0 x 106 cells/ml) were transfected with 6 µg of pCAGGS-NP (1-395) using Polyethyleneimine MAX (Polysciences, Warrington, PA, USA). Three days post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... DexMA was dissolved at 0.5 g mL-1 in a 1:1 mixture of milli-Q water and dimethylformamide with 1% (w/v) Irgacure 2959 photocrosslinker and 0.625 mM methacrylated rhodamine (Polysciences, Inc., Warrington, PA). For matrix displacement studies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 100 µg plasmid in complex with 250 µg PEI MAX 4000 (Polysciences, Inc, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
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bioRxiv - Immunology 2022Quote: ... the next day cells were first transfected with 1 μg/well of the paT7 plasmids using polyethyleneimine (PEI) (Polysciences, Inc. Warrington, PA); 2 hours later ...
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bioRxiv - Cancer Biology 2022Quote: ... or HEK293T cells were seeded for 24h and transiently transfected with the indicated vectors using either polyethyleneimine polymer (Polysciences Inc; 23966-1) TransIT-X2 Dynamic Delivery System (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells grown to 70-80% confluency were transfected with 6 μg ITR-containing plasmid and 22 μg pDGM6 using 112 μg acidified PEI (Polysciences, 23966-1). 48-72 hours after transfection ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2020Quote: ... to 100 μL and then added to 6.0 μg of polyethylenimine (PEI) (1 mg/mL, linear, 25 kDa; Polysciences, Warrington, FL, USA) diluted in PBS to 100 μL (3:1 ratio of PEI to DNA (w/w)) ...
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... Solutions were then placed into fresh tubes along with a single polystyrene bead (1/8 inch Polyballs, Cat. No. 17175; Polysciences, Warrington, PA) and agitated again for 30 min to remove trace hexadecane still present in solution ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... 100-200 µg of purified bacmid in 1 mL water were incubated with 400 µg of 25000 MW polyethyleneimine (PEI; Polysciences Cat# 23966) in 1 mL water at 55 degC for 30-45 minutes to sterilize ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
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bioRxiv - Cancer Biology 2021Quote: ... All whole digestive tract was removed and fixed in PBS and 4% electron microscopic-grade paraformaldehyde (Polysciences, USA) for 35 min ...
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bioRxiv - Developmental Biology 2022Quote: ... TrpA1-KI-Flag and TrpA1 mutant (different days as specified in legend) were fixed in 4% Formaldehyde (Polysciences) in 1XPBS for 2 hours under room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic Müllerian and adult oviducts were dissected in PBS and fixed in 4% w/v PFA/PBS (Polysciences) for 30 minutes at room temperature followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... At 18 DIV Cells were fixed using 4 % PFA for 15 min and mounted on glass slides using Aqua-Poly/Mount (Polysciences Inc., Eppelheim). The experimenter was blinded to all the conditions ...
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bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
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bioRxiv - Bioengineering 2019Quote: Neurons were fixed in methanol-free 4% formaldehyde ultra-pure EM grade (Polysciences, Inc., cat. no. 18814-10) + 4% sucrose in PBS for 20 min at room temperature and permeabilized with 0.2% Triton X-100 for 5-10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed and fixed with 2% ultra-pure formaldehyde (Polysciences, Warrington, PA) for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 μl retrograde tracer Fast Blue (2% w/v in 0.9% saline; Polysciences, Germany) was injected into the operated stifle joints 7 days prior to mouse sacrifice to label knee innervating neurons.
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bioRxiv - Biophysics 2021Quote: ... The water surrounding the spheres was seeded with 2 µm polystyrene spheres (Polysciences 19814). PIV analysis was performed using PIVlab [53] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with polyethylenimine (PEI; linear, MW 25,000; Polysciences, cat. No. 23966-2) at a 2:1 ratio (PEI:DNA ...
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bioRxiv - Immunology 2021Quote: ... Small-scale vector preparations were generated by polyethylenimine or PEI (Polysciences, Cat #24765-2) triple transfection of AC1 or AC3 ITR-flanked transgene ...
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bioRxiv - Biochemistry 2022Quote: ... An aqueous 10µg/ml stock of linear 25kDa PEI (Polysciences, catalogue number 23966-2) was used for the transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... and 19.5 μg of psPAX2 were transfected using polyethylenimine (1μg/μl, Polysciences # 23966-2) into 293T cells plated in 6-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µl of 6-µm-diameter polystyrene MB suspension (containing 6.3 × 106 beads, Polysciences), and 1 µl of PBS containing 30% Healon was injected into the anterior chamber using a 32-gauge needle (Hamilton Company ...
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bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected at a density of 2.5 × 106 / ml with a DNA concentration of 3 μg/ml and polyethyleneimine (Linear PEI 25 kDa molecular weight, PolySciences Inc) at 9 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Immunology 2023Quote: ... 0.5 mM spermidine in 1X protease inhibitor buffer) and incubated with activated concanavalin A (ConA) coated magnetic beads (Polysciences - 86057-3) in PCR strip tubes at room temperature for 10 minutes ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...