Labshake search
Citations for Polysciences :
801 - 850 of 970 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed with 1 μg of DNA per single well of a 6-well plate using PEI MAX (Polysciences, INC.) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... -eG-DECAI-FP or recombined forms of eG-SCON-FP or eG-DECAI-FP) was mixed with 8 μl of polyethyleneimine (1 mg/ ml; Polysciences, 23966) and incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Biophysics 2021Quote: 1×107 cells were seeded in 75cm2 flasks 24 h prior to transfection with Polyethylenimine (PEI 25K, Polysciences Europe GmbH, Germany) at a DNA to PEI ratio (w/w ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared by the addition of 2-10 ug of bacmid DNA to 100 μl 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine “Max” MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were washed 3x 20 minutes in 1% Triton X-100 PBS at room temperature and tissues preserved under coverslips using Aqua-Poly/Mount (Polysciences, 18606). The following primary and secondary antibodies at indicated concentrations were used ...
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bioRxiv - Microbiology 2021Quote: ... Optimization and calibration of the quantification parameters were achieved using yellow-green 1 μm microspheres (441/485 ex/em; Polysciences, PA). Live cyanobacteria were also quantified using Syto9 using FITC filters ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection reactions were prepared by the addition of 2-10 μg of bacmid DNA to 100 μL 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 100 μg plasmid in complex with 250 μg PEI MAX 4000 (Polysciences, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 5.7 μg/150 mm dish of construct DNA was transfected with 22.8 μg/150 mm dish of pAAV9 capsid plasmid and 11.45 μg/150 mm dish of pAdDeltaF6 helper plasmid using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... was co-transfected with a pcDNA5/FRT/TO plasmid containing the gene of interest in a 1:10 ratio using polyethylenimine “MAX” (Polysciences, 24765). After medium exchange 6 h post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... negative staining was performed by depositing for 10 minutes with the gird membrane side down on a drop of 1% (w/v) uranyl acetate (Polysciences, USA). The remaining solution on the grid was removed using filter paper (Whatman ...
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bioRxiv - Bioengineering 2022Quote: ... and gRNA expression plasmid (6 μg) were co-transfected with transfection reagent at a 2.6/1 ratio of Polyethylenimine (PEI; Polysciences, Warrington, USA) (μg)/total DNA (μg) ...
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bioRxiv - Microbiology 2022Quote: ... mice were fasted for two hours and then given a single dose of 1×106 10 μm Fluoresbrite® YG carboxylate microspheres (Polysciences) via oral gavage ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Neuroscience 2022Quote: ... cell nuclei were stained by DAPI for 1 min at RT and tissues were mounted in Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using Zeiss LSM 880 confocal microscope with a Zeiss 20x/0.8 Plan-Apochromat objective and later analyzed using ImageJ.
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bioRxiv - Biochemistry 2024Quote: ... HEK293 and HEK293 β-arrestin1/2 KO cells were transfected in suspension with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 million cells ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was added to the PBS at 1:2500 for nuclear staining) and then mounted on charged glass-slides and cover-slipped in Aqua Poly/Mount (Polysciences, Inc.). Primary antibodies used ...
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bioRxiv - Neuroscience 2024Quote: ... AAV vectors were generated through a transient transfection method utilizing three plasmids and linear polyethylenimine (Polysciences, Warrington, PA, USA, 24765-1) for the process ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected at 60-70% confluence using polyethyleneimine (PEI) as the transfection reagent from Polysciences (cat. no. #24765-1).The cell medium was replaced with conditioned culture medium 5 hours post-transfection ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 × 106 cells ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected with BirAGFP-KIF13B Tail 2 or Tail 3 constructs (designated C2 and C3, respectively, in (Serra-Marques et al., 2020)) using Polyethylenimine (PEI; Mw 2500; Polysciences) at a 3:1 PEI:DNA ratio (w/w) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
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bioRxiv - Immunology 2024Quote: We have previously created FLP using purified fungal carbohydrates which we can associated with amine-coated polystyrene 3 μM microspheres (Polysciences) using either chemical conjugation or adsorption57,58 ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells were transfected with 3 µg pcDNA3 HA-KBTBD4 plasmid and 3 µg pcDNA3 CoREST-FLAG (full-length or truncated) using PEI MAX transfection reagent (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells were transfected with 2 µg pcDNA3 HA-KBTBD4 plasmid (mutant or WT) and with or without 3 µg pcDNA3 CoREST-FLAG (full-length or truncated) using PEI MAX transfection reagent (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μg each of DNA constructs respectively were used with polyethylenimine (PEI; Polysciences, Eppelheim, Germany) as previously described (8 ...
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bioRxiv - Immunology 2022Quote: ... DAPI for 5 minutes and then cover slipping the tissue sections with Aqua-Poly mount (Polysciences). CD25+ cells were detected after the TBS wash step by blocking with 3% H2O2 for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl (10^10 microspheres/mL) of Fluoresbrite® Multifluorescent Microspheres 0.2 µm (PolySciences 24050-5) were spread onto a bilayer plate using sterile glass beads and dried for 30 minutes ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Adeno-associated viruses (AAVs) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Evaluation of phagocytic uptake was performed using Fluoresbrite® BB Carboxylate Microspheres 1.75µm (Polysciences, 17686-5). Opsonization of the microspheres was performed by incubation in 50% FBS in PBS for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS twice for 5 min and mounted with Aqua PolyMount medium (PolySciences). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... Day-one adults were mounted with 100 nm polystyrene beads (Polysciences #00876-15; 5-fold dilution) on an agarose pad ...
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bioRxiv - Neuroscience 2024Quote: ... following previously published protocol.42 Triple transfection was performed with Transporter 5 Transfection Reagent (Polysciences 26008). AAVs were harvested 72 hours post-transfection and purified on iodixanol gradient ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells grown in 10 ml of medium (3.0 x 106 cells/ml) were transfected with 6 µg of pCAGGS-NP (1-395) using Polyethyleneimine MAX (Polysciences, Warrington, PA, USA). Three days post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... DexMA was dissolved at 0.5 g mL-1 in a 1:1 mixture of milli-Q water and dimethylformamide with 1% (w/v) Irgacure 2959 photocrosslinker and 0.625 mM methacrylated rhodamine (Polysciences, Inc., Warrington, PA). For matrix displacement studies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 100 µg plasmid in complex with 250 µg PEI MAX 4000 (Polysciences, Inc, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... the next day cells were first transfected with 1 μg/well of the paT7 plasmids using polyethyleneimine (PEI) (Polysciences, Inc. Warrington, PA); 2 hours later ...
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bioRxiv - Cancer Biology 2022Quote: ... or HEK293T cells were seeded for 24h and transiently transfected with the indicated vectors using either polyethyleneimine polymer (Polysciences Inc; 23966-1) TransIT-X2 Dynamic Delivery System (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells grown to 70-80% confluency were transfected with 6 μg ITR-containing plasmid and 22 μg pDGM6 using 112 μg acidified PEI (Polysciences, 23966-1). 48-72 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...