Labshake search
Citations for Polysciences :
751 - 800 of 845 citations for 6 4 methylpiperazin 1 yl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... or with GFP-tau and vectors pCDNA3 overexpressing Cdk5 and p25 (1:0.85:0.15) using linear Polyethylenimine (PEI; Polysciences, Inc.). Cells were harvested 48 hours after transfection by centrifugation and flash-frozen ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Cell Biology 2019Quote: ... were placed within a of droplet of 1 µl M9 buffer containing 1mM levamisole and 0.1 µm polystyrene microbeads (Polysciences, USA) on a 10 % agarose pad ...
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bioRxiv - Cell Biology 2019Quote: Age-synchronized males (3 d after bleaching adult hermaphrodites fertilized by males) were placed in droplets of 1 µl polystyrene microbeads solution (bead diameter of 0.1 µm; Polysciences, USA) on 10% agarose pads ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Microbiology 2021Quote: ... the construct was expressed in 293F by transfecting with PEIMAX PEI MAX™ (24765-1, Polysciences, Inc., Warrington, PA, USA) according to the manual ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenized mixture was then poured into a solution containing 1% polyvinyl alcohol (25 kDa, 88% hydrolys, Polysciences, Warrington, PA) in water under continuous stirring ...
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bioRxiv - Cell Biology 2020Quote: HEK293T stable cell lines for HSPB8-V5 were transiently transfected with different wild type or mutant BAG3-GFP constructs using PEI (23966-1, PolySciences Europe ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated for 2h at RT and then washed in PBS and further incubated for 30 minutes in 1 ml 50 mM ethanolamine (Polysciences). The beads were then washed 3 times in PBS and left in PBS on a cold room rotator.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cells in a single well of 6-well plate (confluency ≥80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Genetics 2022Quote: Circulating blood monocytes were labeled in vivo by retroorbital intravenous injection of 1 μm Flouresbrite YG microspheres (Polysciences Inc, PA) diluted 1:4 in sterile PBS in mice undergoing regression 3 days prior to harvest ...
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bioRxiv - Microbiology 2019Quote: ... expression medium was added to reduce cell density to 1.0×106 ml-1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Immunology 2020Quote: ... the cells were transfected using 6 μg p509 and 6 μg p2670 plasmid DNAs (Neuhierl et al., 2002) mixed with 72 μl of a 1 mg/ml stock solution of polyethylenimine (#24765; Polysciences) in water (pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Four hundred μl of undiluted culture was then mixed with 50 μl of a 1:100 dilution of 1.75 μm fluorescent microspheres (Polysciences 19392). Four hundred fifty μl of the bead/culture suspension was added to the epithelial surface of individual tissue sections ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Each culture well was transfected with 1 μg of FLAG-LRRK1 construct and 500 ng of EGFP-Rab7 using polyethylenimine (Polysciences) and cultured at 37°C with 5% CO2 for 36 h before harvesting.
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bioRxiv - Genetics 2023Quote: ... or recombined forms of eG-SCON-FRT-FP was mixed with 8 μl of polyethyleneimine (1 mg/ml; Polysciences, 23966). These solutions were then incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Immunology 2023Quote: HEK293T cells at 60-70% confluence were transiently transfected with HIV-1 ADA gp145 Env using polyethylenimine (PEI Max, Polysciences) in a 1:3 ratio of plasmid to PEI (weight to weight) ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Molecular Biology 2023Quote: ... with modified pCW57.1 target vector in a ratio of 5:1:5:5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966) as transfection reagent ...
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bioRxiv - Biochemistry 2023Quote: ... pCAG-FIP200NTD (1-640) was co-transfected with pCAG-TSF-ATG13363-517-ULK1MIT (836-1050) using the polyethylenimine (PEI) (Polysciences) transfection system ...
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bioRxiv - Bioengineering 2024Quote: ... AORBs were then transferred to optimal cutting temperature (Tissue-Tek, OCT) compound (Sakura, 4583) in a cryomold (Polysciences, 18986-1), with as little PBS as possible ...
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bioRxiv - Molecular Biology 2024Quote: One 15 cm plate of HEK293T cells was transfected with the indicated plasmids using Opti-MEMTM (31985062, ThermoFischer) and Polyethylenimine (23966-1, Polysciences), followed by harvest after 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Immunology 2019Quote: ... 293F cells were co-transfected with BG505-ST-710 and furin DNA vectors (500 and 250 µg per 1 L of cells, respectively) using PEI (PolySciences, Inc). The cells were harvested by centrifugation (3,000 RCF ...
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bioRxiv - Genetics 2021Quote: ... -eG-DECAI-FP or recombined forms of eG-SCON-FP or eG-DECAI-FP) was mixed with 8 μl of polyethyleneimine (1 mg/ ml; Polysciences, 23966) and incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Biophysics 2021Quote: 1×107 cells were seeded in 75cm2 flasks 24 h prior to transfection with Polyethylenimine (PEI 25K, Polysciences Europe GmbH, Germany) at a DNA to PEI ratio (w/w ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared by the addition of 2-10 ug of bacmid DNA to 100 μl 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine “Max” MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were washed 3x 20 minutes in 1% Triton X-100 PBS at room temperature and tissues preserved under coverslips using Aqua-Poly/Mount (Polysciences, 18606). The following primary and secondary antibodies at indicated concentrations were used ...
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bioRxiv - Microbiology 2021Quote: ... Optimization and calibration of the quantification parameters were achieved using yellow-green 1 μm microspheres (441/485 ex/em; Polysciences, PA). Live cyanobacteria were also quantified using Syto9 using FITC filters ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection reactions were prepared by the addition of 2-10 μg of bacmid DNA to 100 μL 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...