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Citations for Polysciences :
701 - 750 of 987 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Slides were rinsed before being cover-slipped with mounting media (Mowiol 4-88, Polysciences, 17951) containing an anti-fade reagent (p-Phenylenediamine ...
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bioRxiv - Cell Biology 2020Quote: EVs were incubated overnight (4°C) with polystyrene microspheres 4.5 µm (Polysciences INC, Warrington, PA). The microspheres were blocked with 2% BSA (v/w ...
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bioRxiv - Microbiology 2020Quote: ... and both the apical and basal parts were fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.), and blocked with 10% goat serum (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips with the hippocampal cultures were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Neuronal images were acquired using an Olympus IX81 inverted epifluorescence microscope at 63× optical magnification with a CCD camera (Princeton MicroMax ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed in 4% paraformaldehyde/0.01% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... and both the apical and basal parts were fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.) in 1x PBS for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... The fixed suspension of bacteria was washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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Novel amiloride derivatives that inhibit bacterial motility across multiple strains and stator typesbioRxiv - Microbiology 2021Quote: ... and incubated for 10 mins and washed with motility buffer and flushed with 1 μm polystyrene bead (Polysciences, Inc) solution ...
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bioRxiv - Immunology 2021Quote: ... 480µg of each plasmid was co-transfected with 120µg of BirA (Howarth et al., 2008) and 12mg PEI-Max (1 mg/mL solution, Polysciences) in the presence of 200 µM biotin (final concentration) ...
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and sorting efficiency was verified manually by sorting a specified number of 1-μm yellow-green microspheres (Polysciences, USA) onto a glass slide and counting the beads under an epifluorescence microscope ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear, MW 25,000, 23966-1-A, Polysciences) transfection method (45) ...
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Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was carried out by placing adult worms in 1.5 μl of M9 mixed with 1.5 μl of 1 μm polysterene microspheres (Polysciences Inc.) on a coverslip which was mounted carefully onto a 5% agarose pad.
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... or with GFP-tau and vectors pCDNA3 overexpressing Cdk5 and p25 (1:0.85:0.15) using linear Polyethylenimine (PEI; Polysciences, Inc.). Cells were harvested 48 hours after transfection by centrifugation and flash-frozen ...
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bioRxiv - Neuroscience 2021Quote: ... temporal overlapping calibration of the pump and Stokes beams at the fingerprint and C-H vibration regions was performed by adjusting a built-in delay stage based on the SRS signal of 6 μm polystyrene beads (Polysciences, Inc., Warrington, PA), Olive oil and heavy water (99% pure ...
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bioRxiv - Neuroscience 2019Quote: ... and Madin-Darby canine kidney (MDCK-II) were grown in 6-well plates until 80% confluency and transfected using polyethylenimine (PEI; Polysciences Inc, Warrington, USA) (Cording et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Plat-E cells were seeded into 6-well plates and transfected with pMXs-Venus-IRES-PuromycinR and pMXs-HOXB7-IRES-PuromycinR plasmid DNAs using 6 μg polyethylenimine (PEI; Polysciences, Inc., PA, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Exogenous plasmid DNA was transfected using Transporter™ 5 transfection reagent (Polysciences, PA, USA). Co-transfection of siRNA with plasmid DNA was performed using jetPRIME® transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293T cells were performed using the Transporter 5 Transfection Reagent (Polyethylenimine; Polysciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 2-3μl of 1.25% (w/v) polystyrene microspheres (Polybead Microspheres 0.05μm, Polysciences) and 0.2mM levamisole (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently transferred to 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... were produced as previously described using a PEI (polyethylenimine, Polysciences, 24765-2) transfection protocol followed by iodixanol gradient purification ...
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bioRxiv - Molecular Biology 2019Quote: Overexpression of vectors was performed using Polyethylenimine MAX 4000 (Polysciences, 24885-2) with a DNA:PEI ratio of 1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... and 48 μL of Polyethylenimine ‘Max’ MW 40,000 Da (Polysciences; 24765-2) per plate ...
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bioRxiv - Neuroscience 2023Quote: ... with 2 μl of 100 nm polystyrene beads (Cat# 00876-15, Polysciences) and 2 μl of levamisole (from 500 μM stock ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free FA (Polysciences, 040181) overnight at 4°C and stored in 100% methanol at -20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and chemically fixed by re-suspending in a buffer containing 4% formaldehyde (Polysciences, Inc., Warrington, USA), 80 mM HEPES-K ...
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bioRxiv - Biophysics 2021Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Plant Biology 2021Quote: Material collected for microscopy was fixed in 4% v/v acrolein (Polysciences, New Orleans, Louisiana, USA) in 1X PIPES buffer (50 mmol/L PIPES ...
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bioRxiv - Biophysics 2020Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
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bioRxiv - Cell Biology 2022Quote: ... Larvae were fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2.5% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M sodium-cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...