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Citations for Polysciences :
651 - 678 of 678 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... or HEK293T cells were seeded for 24h and transiently transfected with the indicated vectors using either polyethyleneimine polymer (Polysciences Inc; 23966-1) TransIT-X2 Dynamic Delivery System (Mirus Bio ...
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bioRxiv - Biochemistry 2021Quote: ... Stable cell lines expressing N-terminal Flag tagged receptor constructs in pcDNA3.1 vector were generated by transfecting HEK-293 cells with 7 μg of plasmid DNA using polyethylenimine (PEI) (Polysciences, Cat. no. 19850), followed by selection using G418 (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Plat-E cells were co-transfected with target gene vectors and an amphotropic envelope vector using polyethylenimine (PEI; Polysciences, Warrington, PA, USA) as described previously39 ...
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... cells on every dish were transfected with 15 μg of pcDNA3-HA-BACH1 using the PEI MAX reagent (24765, Polysciences, Warrington, PA). At 48 h after transfection ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2.0×106 cells/ml and co-transfected with pMlink-N-FLAG-p22phox-NOX2 and pMlink-EROS using PEI 4000 reagent (Polysciences, Warrington, PA). The transfected cells were cultured for 48 h and collected by centrifuged (4,000 × g ...
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bioRxiv - Biochemistry 2023Quote: HEK293T cells cultured in DMEM with 10% Fetal Calf Serum were transfected using linear polyethylenimine (MW ∼25,000, Cat#23966 Polysciences Inc., Warrington, PA), 40 µg/ 10 cm dish and plasmid DNA ...
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bioRxiv - Cell Biology 2024Quote: Lentiviral vectors were produced by co-transfecting the lentiCRISPR v2 containing the sgRNA with pSPAX2 and VSVg vectors in HEK293T cells using polyethylenimine (PEI - 23966-100 - Polysciences Europe GmbH) in a 1μg:2μL DNA:PEI ratio ...
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bioRxiv - Biophysics 2020Quote: ... 1 L of cells at 1×106 cells were transfected with complexes composed of 250 μg of plasmid DNA and 750 μg of polyethylenimine (Polysciences, Cat. # 24765-1). For the expression involving kifunensine ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of pHEF-VSV-G into 70% confluent monolayers of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Microbiology 2022Quote: ... Vero/TMPRSS2 cells were transfected with pCAGGS-blast-RcACE2 or pCAGGS-blast-hACE2 plasmids using the PEI MAX transfection reagent (Polysciences, Warrington, PA, USA). Transfected cells were treated with 10 μg/mL blasticidin S (Kaken Pharmaceutical ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was produced in HEK293T cells by transfecting plasmid DNA of interest with packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences, Cat# 23966-1). Media were replaced 6-8 hrs following transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ug pHelper plasmid into one 15-cm dish of confluent HEK-293T cells using PEI-Max (Polysciences Inc. catalog # 24765-1). After transfection the next day we changed the medium to 1% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cancer Biology 2021Quote: High titer lentivirus was generated by transfecting HEK293FT cells with a mixture containing three plasmids and 25-kDa linear polyethyleneimine (PEI) (Polysciences, Inc. PA, USA, 239662). In brief ...
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bioRxiv - Cancer Biology 2020Quote: Lentivirus was produced in HEK293T cells by transfecting plasmids together with helper packaging plasmids (VSVG and psPAX2) using polyethylenimine (PEI 25000; Polysciences; Cat. No. 23966-1) transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... 1.5 × 108 cells expressing GFP-Nup131 were fixed in 1 mL of a mixture of 4% formaldehyde (18814-10, Polysciences, Inc, Warrington, PA, USA) and 0.01% glutaraldehyde (1909-10 ...
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bioRxiv - Cancer Biology 2022Quote: ... These vectors along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293FT cells by polyelthylenimine (Polysciences, Inc; 60 μg/mL) mediated transfection ...
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bioRxiv - Neuroscience 2024Quote: ... FreestyleTM 293F cells were transiently transfected with the mixture of DNA plasmid and polycation polyethylenimine (PEI; 25 kDa linear PEI, Polysciences, Inc., cat. No. 23966). The transfected cells were incubated at 125 rpm in FreestyleTM 293 Expression Medium for suspension culture at 37°C with 5% CO2 ...
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bioRxiv - Genetics 2024Quote: ... 1 million HEK 293T cells in a single well of a 6-well plate were transfected using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 1 μg of PsPax2 (Addgene # 12260) ...
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bioRxiv - Evolutionary Biology 2023Quote: 1,5×105 Cells were seeded on 15 mm round glass coverslips coated with polyethylenimine (PEI) solution (25 g/ml PEI, Polysciences Inc. and 150 nM NaCl) for 48 hours ...