Labshake search
Citations for Polysciences :
601 - 650 of 650 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Microbiology 2022Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells using PEI Max (Polysciences, Cat# 24765-1). At 48 h posttransfection ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 min and coverslips were mounted onto SuperFrost® Plus slides (R. Langenbrink) with fluorescent Aqua-Poly/Mount mounting medium (Polysciences, 18606-20). The cells were imaged using a Leica TCS SP5 confocal laser scanning microscope and an HCX PL APO lambda blue 63× oil UV objective or HCX PL APO 40.0×1.30 OIL objective controlled by the LAS AF scan software (Leica Microsystem ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were seeded for 18–24 hr and transiently transfected with the indicated vectors using either the JetPrime reagent (Polyplus-transfection; 114-15) or polyethylenimine polymer (Polysciences Inc; 23966-1), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.625 µg of pMD2.G) using 15 µL of 1 mg/mL transfection grade linear polyethylenimine hydrochloride (MW 40,000) (PEI-MAX-40K) (Polysciences, Warrington, PA, United States) in 500 µL of Opti-MEM ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ug pHelper plasmid into one 15-cm dish of confluent HEK-293T cells using PEI-Max (Polysciences Inc. catalog # 24765-1). After transfection the next day we changed the medium to 1% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with the transfection solution that was prepared by combining 8 µL (per dish, hereafter) polyethyleneimine (PEI) solution (1 mg/mL; Polysciences, Warrington, PA, USA) and a plasmid mixture consisting of 400 ng human LPAR-encoding plasmids ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was produced in HEK293T cells by transfecting plasmid DNA of interest with packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences, Cat# 23966-1). Media were replaced 6-8 hrs following transfection ...
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bioRxiv - Immunology 2023Quote: ... Thermo Fisher Scientific) for 1 hour after which the coverslips were mounted on slides with aqua-poly/mount (Aqua-Poly/Mount, Polysciences, Warrington, PA, USA) and analyzed by fluorescence microscopy ...
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bioRxiv - Immunology 2024Quote: ... we labeled circulating monocytes by injecting the mice retro-orbitally with 1 µm diameter Flouresbrite flash red plain microspheres beads (Polysciences Inc, Warrington, PA) diluted in sterile PBS 24 hours before harvesting ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... before the ROS generating agent 2,2’-Azobis(2-amidinopropane) dihydrochloride (AAPH; final conc. 1 mM in media; 08963, Polysciences, Inc., Warrington, PA, USA) was added for over-night incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... four L square glass bottles each containing 200 mL of suspension culture at ∼2.5 × 106 cell/mL were transfected using 1 µg/mL DNA and 3 µg/mL polyethylene ‘Max’ 40 kDa (Polysciences, Warrington, PA, #24765). The pre-premixing of DNA and PEI-Max was incubated for 20 min at room temperature before added to the bottles ...
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Cancer Biology 2020Quote: Lentivirus was produced in HEK293T cells by transfecting plasmids together with helper packaging plasmids (VSVG and psPAX2) using polyethylenimine (PEI 25000; Polysciences; Cat. No. 23966-1) transfection reagent ...
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bioRxiv - Neuroscience 2023Quote: ... Transfection with the plasmid expressing PH-PLCδ::GFP probe was done 24 hours before the imaging experiments using Polyethylenimine solution (PEI; Polysciences Inc.; 1 mg/ml) when the cells were around 70% confluent.
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bioRxiv - Immunology 2023Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) were incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... individual animals were placed in a drop of 8 ul of 1 mM levamisole in phosphate buffered saline and diluted 25 um diameter Polybead® Polystyrene beads (PolySciences Inc, Warrington, PA). After addition of a coverslip ...
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bioRxiv - Cancer Biology 2024Quote: ... Warm cross-link reversal buffer (200 µl 800 mM Tris-HCl pH8.0) was added followed by addition of 6 µl of 1:10 Biomag amine paramagnetic beads (48 mg/ ml, Polysciences cat. no. 86001-10). Homogenization was repeated ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ug of the MAXI-prep DNA that was pre-diluted in 13 ml of Opti-MEM® I Reduced Serum Medium (Thermo, 31985-062) and 800 ug Polyethylenimine (PEI 25K, Polysciences CatNo 23966-1) likewise pre-diluted in 13 ml of Opti-MEM media ...
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bioRxiv - Immunology 2024Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) and incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Neuroscience 2020Quote: IgSF8-Fc and Tenascin-R-Fc proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Systems Biology 2021Quote: ... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids for these proteins were co-transfected with AnkR-GFP in HEK293T cells using PEI Max (Polysciences, 24765) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 µm diameter or Dynabeads T1: 1 µm diameter) at concentrations of 5×106/µl (high) or 5×105/µl (low) or polystyrene beads (Polysciences, 64090-15: 6 µm diameter) at concentrations of 1×105/µl (high ...
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bioRxiv - Pathology 2022Quote: The blood flow during vascular colonization was monitored by perfusing 1 μm diameter fluorescent microspheres (Yellow/Green Fluoresbrite carboxylate, Polysciences, 107 microspheres/ml 1x PBS) at a rate of 15 μl/min before each z-stack series and images acquired on a single plane at high speed (50 frames per second ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc., cat# 08791–500; Dodecenylsuccinic anhydride (DDSA, Polysciences Inc., cat# 00563–450), nadic methyl anhydride (NMA ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Immunology 2020Quote: Samples from NHP were depleted using purified DENV or BSA control protein adsorbed onto Polybeads polystyrene microspheres (Polysciences, Inc.) as reported earlier (11 ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Molecular Biology 2024Quote: The pcDNA3.4 plasmid containing His-tagged SARS-CoV-2 spike protein was transfected to Expi293T cells using PEI max (Polysciences). After 18–21 h post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... with rat GRIK1-1aEMplasmid [or baculovirus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Molecular Biology 2022Quote: Proteins were produced by transient transfection of plasmids into HEK 293F cells using the polyethylenimine (PEI) Max reagent (Polysciences, Inc.) with a 1:3 ratio (wt/wt ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Genomics 2024Quote: ... CaGHV-1 RTA was expressed as an N-terminally 3xFLAG-tagged protein in HEK293T cells by transfecting the cells with PEI (Polysciences). The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega ...
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bioRxiv - Biophysics 2021Quote: ... in a microcentrifuge tube and resuspending in coupling buffer (400 μl in first wash and 170 μl in second washing) (PolyLink Protein Coupling Kit, Polysciences Inc.). Then 20 μl of the freshly prepared EDCA solution (20 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen sections and plated cells were mounted following antibody staining using AquaPolymount (Polysciences, Heidelberg, Germany) and imaged using a Zeiss Apotome ImagerZ2 (Zeiss ...
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bioRxiv - Immunology 2020Quote: ... BioInvent n-CoDeR® scFv were selected using biotinylated in-house produced recombinant LILRB3-hFc recombinant fusion proteins (captured with streptavidin-coated Dynabeads®) with or without competition or LILRB1-hFc coated to etched polystyrene balls (Polysciences, US) or plastic immunotubes ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells at 70% confluency in 15-cm culture plates were transfected with pFUW-GFP-AR, along with viral packaging and envelope proteins (pRSV/REV, pMDLg/RRE, and pVSV-G) using polyethylenimine transfection reagent (Polysciences; cat. #23966). 48 h post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... single-round HIV-1 Env-/Vif-/Vpr-/Nef-/CFP reporter virus with 1ug plasmid encoding the G protein from vesicular stomatitis virus (VSV-G) for virion pseudotyping) in 10cm dishes using polyethylenimine (PEI; catalog no. 23966; Polysciences Inc., Warrington, PA, USA). Culture media were replaced at 24 hours post-transfection ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...