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Citations for Polysciences :
551 - 600 of 651 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... at a density of 2.5 × 106 cells/ml were transfected with microbody expression vector plasmid DNA using polyethyleneimine (Polysciences, Inc) at a 1:3 plasmid:PEI ratio ...
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bioRxiv - Microbiology 2019Quote: ... 3×105 cells/well (12-well plate) or 2×106 (10-cm dish) and transfected on the following day using polyethylenimine (PolySciences).
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bioRxiv - Immunology 2020Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Biochemistry 2022Quote: Recombinant truncated human TSEN complexes were produced in human embryonic kidney (HEK) 293F cells by cotransfection using linear polyethyleneimine (PEI) (MW 25000, Polysciences) and incubated for 72 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: We transfected lentiviral plasmids together with gag-pol (pCMV Δ R8.91) and V-SVG (pMD.G VSVG) expressing vectors into HEK293T cells using PEI transfection reagent (Polysciences, #24765). We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... pCS2-3xFlag-SLP-L or pCS2-3xFlag-SLP-S was transfected into HEK293T cells by polyethylenimine (PEI) MAX (Polysciences, 24765). Cells were harvested 24-hour post-transfection and lysed by RIPA cell lysis buffer (Beyotime ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV2-SynTetOff-mRFFP1-BGHpA and helper plasmids were transfected into HEK293T cells (RCB2202, RIKEN BioResource Research Center) with polyethylenimine (23966, Polysciences). Virus particles collected from the cell lysate and supernatant were purified by ultracentrifugation with OptiPrep (1114542 ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...
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bioRxiv - Microbiology 2022Quote: ... we used the ratio of the median forward scatter of each cell population to that of the median forward scatter of reference beads (Polysciences™ ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were produced by transient transfection of plasmids into HEK 293F cells using the polyethylenimine (PEI) Max reagent (Polysciences, Inc.) with a 1:3 ratio (wt/wt ...
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bioRxiv - Biophysics 2022Quote: ... The mutant expression vector was co-introduced with pAc-mfT1r2aL-Ce (Nango et al., 2016) to Drosophila S2 cells using Polyethyleneimine (PEI) “MAX” (Polysciences) as previously described (Bleckmann et al ...
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bioRxiv - Microbiology 2022Quote: ... and HNRNPUL1-V5 or MECR-V5 were forward transfected into 293T cells using PEI (PEI MAX, Polysciences; 6-well plates). Cells were lysed in co-IP buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... one day after plating 2ug of each plasmid (maximum 8 ug of plasmid) was transfected into cells using 60 ug of PEI max (Polysciences). Producer cells were then maintained for 3 days after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... pUCmini-iCAP-PHP.eB and pHelper plasmid with the plasmid ratio of 1:4:2 based on micrograms of DNA were transfected into cells at >90% confluency with 1mg/ml polyethyleneimine (PEI, Linear, MW 25000, Polysciences). pUCmini-iCAP-PHP.eB for PHP.eB production was a gift from Dr ...
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bioRxiv - Molecular Biology 2024Quote: One 15 cm plate of HEK293T cells was transfected with the indicated plasmids using Opti-MEMTM (31985062, ThermoFischer) and Polyethylenimine (23966-1, Polysciences), followed by harvest after 48 hours ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Genomics 2023Quote: ... Expi293F cells at a density of 3×106 cells/mL were transfected with 1mg/L of culture of plasmid complexed with PEI Max 40K (cat. 24765-100, Polysciences) in a 1:2 plasmid:PEI w/w ratio in 40mL Opti-MEM (cat ...
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bioRxiv - Molecular Biology 2023Quote: For HIV-1 virus growth assay pNL4-3 and its mutated versions pNL4-3 TC-1G and pNL4-3 TC-3G were transfected into freshly seeded 293T cells using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the constructed plasmid using polyethyleneimine (Polysciences). Transfected cells were imaged using a IX83 wide-field fluorescence microscopy (Olympus ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Immunology 2023Quote: HEK293T cells at 60-70% confluence were transiently transfected with HIV-1 ADA gp145 Env using polyethylenimine (PEI Max, Polysciences) in a 1:3 ratio of plasmid to PEI (weight to weight) ...
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bioRxiv - Biochemistry 2023Quote: ... to cells was 1 µg DNA for 106 cells and the ratio of total DNA to PEI Max transfection agent (Polyethylenimine HCL MAX 40 K linear, Polysciences) for 1 µg DNA for 3 µg transfection agent ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding for HIV Env SOSIP trimers were cotransfected in HEK293F cells using PEI-MAX 4000 transfection reagent (Polysciences, Inc.). After transfected cells were incubated for four days ...
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bioRxiv - Microbiology 2024Quote: ... vesicular stomatitis virus G (VSV-G)-pseudotyped HIV-1 viruses were produced by co-transfection of HEK293T cells with an HIV-1 proviral construct and a VSV-G-encoding vector using the PEI reagent (Polysciences). Two days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: HEK293 or HEK293T cells were transfected with plasmids expressing GFP-fused NCAN using Lipofectamine 3000 (Thermo) or PEI Max (Polysciences). After three or four days ...
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bioRxiv - Molecular Biology 2024Quote: ... with modified pCW57.1 target vector in a ratio of 5 : 1 : 5 : 5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966). Three days post-transfection ...
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bioRxiv - Immunology 2022Quote: ... the pCAGGS-RBD plasmid was transfected into ∼5×107 adherent HEK-293/T17 cells (ATCC CRL-11268) in a T-175 using PEI (Polyethylenimine, linear 25,000 m.w.(Polysciences, Warrington, PA, USA). Plasmid was mixed at a 1:3 ratio with PEI (20ug of plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mm dishes of HEK293T cells (∼80% confluent) were transfected with 10 μg of plasmid using 60 μg of linear Polyethylenimine 25 kDa (23966, Polysciences Inc) mixed in 2 mL Opti-MEM (31985070 ...
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bioRxiv - Immunology 2019Quote: ... 293F cells were co-transfected with BG505-ST-710 and furin DNA vectors (500 and 250 µg per 1 L of cells, respectively) using PEI (PolySciences, Inc). The cells were harvested by centrifugation (3,000 RCF ...
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bioRxiv - Biophysics 2021Quote: 1×107 cells were seeded in 75cm2 flasks 24 h prior to transfection with Polyethylenimine (PEI 25K, Polysciences Europe GmbH, Germany) at a DNA to PEI ratio (w/w ...
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bioRxiv - Developmental Biology 2022Quote: ... The CSII-CMV-MCS-IRES2-Bsd vector (Fbln7 FL, dCC or control eGFP) was transfected into 293T cells using polyethylenimine reagent (PEI, Polysciences Inc.) together with lentivirus packaging vectors (pRSV-Rev ...
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bioRxiv - Biochemistry 2022Quote: ... The two constructed pSpCas9(BB)-2A-Puro (PX459) vectors against individual genes were transiently transfected into HCT116 cells in a six-well plate using polyethyleneimine “Max” (Polysciences, Inc.). At 48 h post-transfection ...
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bioRxiv - Biophysics 2020Quote: ... the plasmids pCAGGS-SARS2-S and pCAGGS-SARS-S were transiently expressed on HEK 293T cells using Polyethylenimine (PEI) (Polysciences, USA). 24 h post-transfection ...
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bioRxiv - Immunology 2020Quote: ... pPPI4 DNA vectors carrying free SOSIP trimers or SOSIP-based antigen-bearing components were transfected into FreeStyle 293F cells using polyethyleneimine (Polysciences, Inc) as described previously(6) ...
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bioRxiv - Cell Biology 2022Quote: 200,000 HeLa cells/35-mm well were seeded onto coverslips (Marienfeld, 630-2190) and transfected using polyethylenimine transfection reagent (PEI, Polysciences, 23966) in a 1:6 (w/w ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... and HAP1 cells were initially tested for absence of mycoplasma contamination (UC Berkeley Cell Culture facility) by fluorescence microscopy of methanol fixed and Hoechst 33258 (Polysciences, #09460) stained samples ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Hek293T-cells were transfected with pNL4-3 or pCH058/2960 or pNL4-3 and pMM310 (pBlam-Vpr) via polyethylenimine (Polysciences Inc.)[67] ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Immunology 2020Quote: ... 1 milligram of plasmid DNA encoding HIV-1 Env per 1 liter of cells was diluted in DMEM and mixed with polyethyleneimine (PEI, Polysciences, Inc.). The DNA was allowed to complex with PEI for 25 min and then cells were added ...
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bioRxiv - Neuroscience 2022Quote: ... vectors were transfected to 50-80% confluent HEK293T cells in 35-mm dish using PEI-MAX (25 mM, 5 μL/dish; Polysciences, Inc.). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then transfected with 1 μg of JFH1-QL/GLuc2A in vitro transcribed RNA (1 μg) using polyethylenimine (Polysciences, Inc.). Supernatants were harvested and mixed with Renilla luciferase buffer and substrate ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Neuroscience 2022Quote: ... cell nuclei were stained by DAPI for 1 min at RT and tissues were mounted in Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using Zeiss LSM 880 confocal microscope with a Zeiss 20x/0.8 Plan-Apochromat objective and later analyzed using ImageJ.