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Citations for Polysciences :
551 - 600 of 1041 citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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bioRxiv - Physiology 2020Quote: ... animals were post-fixed with 1% aqueous solution of osmium tetroxide (Polysciences Europe GmbH 0972B-5) and 1.5% potassium ferrocyanide (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Calculations of relative fluorescence per cell were done by normalizing red chlorophyll fluorescence per cell to 2 μm reference fluorescent beads (catalog no. 18604; Polysciences, Warrington, PA, USA) as previously described [72] ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2019Quote: ... We then sterilized 1- and 6-µm diameter polystyrene microbeads (Polysciences, Cat#: 07310-15 and 07312-5) as noted in Cone et al. ...
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bioRxiv - Biophysics 2022Quote: ... The transfection solution was prepared by combining 5 μL of 1 mg/ml polyethylenimine Max solution (Polysciences) and a plasmid mixture consisting of 200 ng ssHA-FLAG-NTS1 and 500 ng alkaline phosphatase (AP)-tagged TGF-α (AP-TGFα ...
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bioRxiv - Cancer Biology 2024Quote: ... Fast Blue (5%, Polysciences) or rAAV-hSyn-EGFP (1012vp/ml ...
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bioRxiv - Neuroscience 2021Quote: ... or Aquamount (#18606-5, Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, 111-30-8) in 0.01 M PBS (Fisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescent polychromatic beads (Polysciences, 19507-5) with a 0.5 μm particle size were added to each well at a final concentration of 3.64×108 beads/mL and incubated at 20°C for 10 minutes with shaking ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 475 ng either of pCAG-hSTAT6 (VT→AA, Y→F) or pcDNA was transfected by using PEI MAX (Polysciences, Warrington, PA, USA) according to the manifacture’s guide ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
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bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
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bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
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bioRxiv - Systems Biology 2022Quote: ... pMDLg/pRRE) and 8 ug of rTetR-domain library vectors using 50 mL of polyethylenimine (PEI, Polysciences #23966). pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Synthetic Biology 2022Quote: ... pMDLg/pRRE) and 8 ug of rTetR-concatenation library vectors using 50 mL of polyethylenimine (PEI, Polysciences #23966). Packaging plasmids were gifts from Didier Trono ...
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bioRxiv - Microbiology 2020Quote: ... pCAG-HIVgp (5 μg) and pCMV-VSV-G-RSV-Rev (5 μg) (Riken BioResource Research Center) using PEImax solution (60 μL) (Polysciences) prepared in KnockoutDMEM/F-12 (500 μL ...
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bioRxiv - Biochemistry 2020Quote: ... 5 µm Polybead carboxylate microspheres (Polysciences, Warrington, PA) were covalently coupled with goat anti-human pAb using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/EDC chemistry (49) ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids and PEI (product #260008-5; Polysciences) were separately diluted in Opti-MEM1 (product #31985-047 ...
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bioRxiv - Cell Biology 2024Quote: ... Polystyrene microspheres (Polysciences #00876-15; 5-fold dilution) were used to immobilize late-L4 animals on 10% agarose pads ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were collected and mounted in 8-10 μL of egg buffer supplemented with 18.8μm polystyrene beads (Polysciences, Inc.).
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were transfected in 100 mm dishes using 8 µg plasmid DNA and 24 µl PEI (Polysciences, Inc). DNA was mixed with PEI in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected at a confluence between 50 % and 70 % with 8 μg of plasmid DNA per dish using polyethyleneimine 25,000 MW (Polysciences) solution as described previously (Guaitoli et al. ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were dissected in 8-10 μL of Shelton’s Growth Medium (Edgar and Goldstein, 2012) and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip ...
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bioRxiv - Synthetic Biology 2024Quote: ... one day after plating 2ug of each plasmid (maximum 8 ug of plasmid) was transfected into cells using 60 ug of PEI max (Polysciences). Producer cells were then maintained for 3 days after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... were transfected between 50% and 70% confluence with 8 µg of plasmid DNA/ 14 cm culture dish using polyethyleneimine (PEI) 25 kDa (Polysciences), and cultured post-transfection for 48 hrs in 14 cm dishes in DMEM (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... 5% agarose pads and 0.1 μm polystylene beads (Polysciences) as described (Niwa ...
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bioRxiv - Immunology 2023Quote: ... at 5 ×106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...
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bioRxiv - Genomics 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery96 ...
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bioRxiv - Immunology 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...
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bioRxiv - Neuroscience 2023Quote: ... or FastBlue (FB, 5% in in 0.9% saline, Polysciences), which was iontophoretically injected (2/2 s on/off duty cycle ...
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bioRxiv - Neuroscience 2020Quote: ... cells were transfected with 8 μ of plasmid DNA (SF-tagged LRRK2 WT, or SF-tagged LRRK2 G2019S) per 10cm2 dish using polyethyleneimine (Polysciences) solution ...
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bioRxiv - Genetics 2020Quote: ... and then transfected with 8 μg of an equimolar mixture of the three third-generation packaging plasmids and 8 μg of rTetR-domain library vectors using 50 μl of polyethylenimine (PEI, Polysciences #23966). After 48 hours and 72 hours of incubation ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared mixing 8-12 μg of bacmid DNA in 100 μL 1xPBS and 12 μg polyethyleneimine (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) in 100 μL 1xPBS and the reaction was allowed to proceed for 20-30 minutes before addition to an Sf9 monolayer containing well ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection reactions were prepared mixing 8-12 μg of bacmid DNA in 100 μL 1xPBS and 12 μg polyethyleneimine (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) in 100 μL 1xPBS and the reaction was allowed to proceed for 20-30 minutes before addition to an Sf9 monolayer containing well ...
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bioRxiv - Biochemistry 2021Quote: ... Expression plasmids (Nsp1/Nsp2/N) and PEI (Polysciences, 260008-5) were separately diluted in Opti-MEM1 (Gibco 31985-047 ...
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bioRxiv - Biophysics 2020Quote: ... Transporter 5™ transfection reagent (PEI MAX) was from Polysciences Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... followed with mounting in Aqua-Poly/Mount (Polysciences, 18606-5).
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bioRxiv - Cell Biology 2023Quote: ... and embedded in Mowiol (Polysciences cat. No. 9002-89-5) containing anti-fading agent DABCO (Merck) ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with the indicated construct using PEI (Transporter 5, Polysciences), then cells were collected two days post transfection with 10 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... using the polyethylenimine(PEI)-based Transporter 5 transfection reagent (Polysciences, #26008) and a 2nd generation viral packaging system comprising gag/pol/tat/rev (psPAX2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were transfected in DMEM supplemented with 5% FBS using polyethylenimine (Polysciences). Media was changed after 4 to 6 h and cells were stimulated with light starting 24 h after transfection for the durations specified below and at the intensities specified in the main text.
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bioRxiv - Evolutionary Biology 2019Quote: ... Animals were exposed to 0.5 μm fluorescent microspheres (Polysciences, cat. # 19507-5) at a final concentration of 7.28 × 108 particles/mL and 5 μL of 1 mg/mL HB101 bacterial lysate to promote feeding for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and inverted onto 5 µL of Aqua-Poly/Mount (Polysciences, Warrington, PA) on a slide ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were mounted with glass coverslips using Aqua Polymount (Polysciences, 18606-5). Images were taken using a Leica SP8 confocal microscope or a Keyance fluorescence microscope and processed using ImageJ (Fiji) ...
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bioRxiv - Immunology 2023Quote: ... 500 μl of Fluoresbrite YG Carboxylate Microspheres 1.75 μm (PolySciences, 17687-5) were opsonized in 4500 μl of Normal Human Serum (GeminiBio ...