Labshake search
Citations for Polysciences :
551 - 600 of 1047 citations for 6H 1 3 Thiazin 6 one 2 ethoxy 4 hydroxy 5 nitro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
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bioRxiv - Microbiology 2019Quote: ... were co-transfected with 3 µg Gibson Assembly product DNA plus 330 ng pHEF-VSV-G using polyethylenimine (Polysciences, Inc., Warrington, PA) at a ratio of 1 µg total DNA to 4 µg polyethylenimine in 800 µl of 150 mM NaCl [72] ...
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bioRxiv - Physiology 2024Quote: ... The balloon was inflated after the first diagonal branch of the LAD in each animal and 3 ml polystyrene microspheres (Polybead, 90 μm, Polysciences, Warrington, PA) were slowly injected via the lumen of the angioplasty catheter ...
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bioRxiv - Biophysics 2020Quote: ... Beaded cantilevers were generated by first drying 6.0 μm carboxylate beads onto a coverslip (17141-5, Polysciences, Inc); a small amount of UV-curable glue (NOA81 ...
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bioRxiv - Molecular Biology 2021Quote: ... was transfected into each 10 cm dish of HEK293T cells together with the packaging vectors psPax2 (7.5 μg) and pCMV-VSV-G (5 μg) using PEImax (67.5 μg; Polysciences) or Lipofectamine-2000 (50 μl ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with 5 µg plasmid DNA and 15 µg Polyethylenimine (PEI 25K™, Polysciences, 23966-100), both previously prepared in OptiMEM (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
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bioRxiv - Neuroscience 2024Quote: Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Developmental Biology 2021Quote: Adult guts were dissected in PBS and fixed twice during 20 min in fresh 4% paraformaldehyde diluted in PBS (18814-10, Polysciences) with a 10 min wash in PBT 0,1% (PBS + Triton X100 Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Developmental Biology 2019Quote: Flies of both genders were raised as described above and larvae from second instar to pre-pupal stages collected and dissected directly into fresh 4% EM grade paraformaldehyde solution (from a 16% stock. Polysciences) in PBS with 0.3% TritonX-100 then incubated for 25 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2020Quote: ... The pore size of isotropic and anisotropic mineralized collagen scaffolds was analyzed following a JB-4 (Polysciences, Inc., Pennsylvania, USA) embedding procedure 30,47 ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... and either fixed for 4 h in 4% PFA in PBS at 4 °C and cryopreserved in 20% sucrose in PBS overnight at 4 °C or directly cryopreserved and then frozen in OCT compound (Polysciences). Sections were prepared at 14 μm ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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bioRxiv - Synthetic Biology 2019Quote: ... A master stock of PEI was made by dissolving linear PEI (Polysciences Cat. No. 23966-2) in deionized water at a concentration of 0.323g/L ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1 µg per one million cells of the plasmids containing CMV promoter and 3×FLAG-tagged geneswere mixed with a three-fold excess (w/w) of polyethylenimine MAX 40 kDa (Polysciences, Warrington, PA, USA) in 4 ml OptiPro (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were incubated for 3 h at RT in the mixture of EtOH and LR White resin (Polysciences, Inc., Warrington, PA, USA) in the following proportions ...
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bioRxiv - Microbiology 2022Quote: ... followed by a 1:2 mixture of propylene oxide for and Polybed 812 epoxy resin for 3 hours and transferred to 100% resin to embed overnight (Polysciences, Inc.,Warrington, PA). Ultrathin sections (70-80 nm ...
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bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Bioengineering 2020Quote: ... and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al. 2020) with 24 μg linear PEI (Polysciences Inc., Warrington, PA) for 6 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and 10 ng of the TetR reporter plasmid pTS1017) was added into 300 μL DMEM and mixed with 4 μg of polyethyleneimine (Polysciences Inc.). The transfection mixture was vortexed and incubated for 20 min before adding into the inner 60 wells of the cell culture plate ...
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bioRxiv - Bioengineering 2020Quote: The pore size of mineralized collagen and mineralized collagen-amnion scaffolds was analyzed following a JB-4 (Polysciences, Inc., Warrington, PA) embedding procedure (9,25) ...
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bioRxiv - Physiology 2021Quote: ... The 4-μm bone sections were stained with the von Kossa method and counterstained with tetrachrome (Polysciences Inc., Warrington, PA, USA). The 8-μm sections remained unstained to measure fluorochrome-based indices of bone formation.
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Cell Biology 2020Quote: ... various stages of the transgenic animals were mounted on 5 % agar pads with polystyrene nanoparticles (Polysciences, 2.5 % by volume) as previously described43 and imaged at an AxioImager M.2 fluorescence microscope (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: AAV8 viruses were produced in HEK293FT cells grown on 5 plates (linear PEI, Polysciences Europe Cat. No. 23966-100) or 10 plates (branched PEI ...
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bioRxiv - Neuroscience 2023Quote: ... Worms were mounted with cover glass on 5% agarose pads with a drop of polystyrene beads (Polysciences 00876-15) as a friction raising agent ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counterstained with DAPI (nuclear stain) in PBS and mounted with Aqua-Poly/Mount medium (18606-5, Polysciences). Representative pictures for figures were exported from whole slide scans using the snapshot function of CaseViewer (Build 2.4.0.119028).
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed using 3% formaldehyde and fixed cells were placed on poly-L-lysine coated multi-well slides (Polysciences Inc., Warrington, PA, USA). Cells were then blocked for one hour using phosphate-buffed saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Microbiology 2019Quote: ... followed by 1 h of fixation in 1% osmium tetroxide (Polysciences Inc.). After extensive wash with dH20 ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...