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Citations for Polysciences :
501 - 550 of 664 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Microbiology 2020Quote: Constructs were introduced into HEK293F cells by high-density transfection with PEI MAX 40K (Polysciences, Inc., Warrington, PA, USA) (47 ...
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bioRxiv - Immunology 2020Quote: ... some of the prepared BALF cell spots were fixed and treated with Wright-Giemsa stain (Polysciences, Inc., Warrington, PA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The pTT5-VHH72hFc1X7 plasmid was transiently expressed in CHO55E1 cells (34) using PEI-Max transfection reagent (Polysciences, Warrington, PA) and a slightly modified protocol as described previously (35) ...
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Microbiology 2022Quote: Soluble PCDH1 variants cloned into pcDNA3.1 (see above) were expressed in 293F cells in shaker flasks by transient transfection with linear polyethyleneimine (Polysciences), as described previously (56) ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counterstained with DAPI (nuclear stain) in PBS and mounted with Aqua-Poly/Mount medium (18606-5, Polysciences). Representative pictures for figures were exported from whole slide scans using the snapshot function of CaseViewer (Build 2.4.0.119028).
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bioRxiv - Biochemistry 2024Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the eLACCO2.1 and deLACCO1 using polyethyleneimine (Polysciences) prior to imaging ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: AAV8 viruses were produced in HEK293FT cells grown on 5 plates (linear PEI, Polysciences Europe Cat. No. 23966-100) or 10 plates (branched PEI ...
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bioRxiv - Neuroscience 2023Quote: ... medium was reduced to half and cells were transfected with a mixture of DNA/polyethyleneimine (PEI) complexes (MW40000, PolySciences). Complexes were formed by combining 500 ng of plasmid DNA in 10 μL of serum free DMEM and 3 μL of PEI (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated to 1.5 x 105 cells per well in 12-well plates 24 hours prior to transfection with polyethylenimine PEI (Polysciences). The primary transfection mixtures contained 125 ng pFLuc-TAR-140 reporter plasmid ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with shRNA constructs and packaging plasmids (psPAX2 and VSV-G) using PEI (polyethylenimine MW25,000, Polysciences). Viral supernatants were collected at 48h and 72h post-transfection and mixed ...
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bioRxiv - Cell Biology 2023Quote: ... lentiviral vector was co-transfected with pSPAX2 and pMD2.G in 293T cells using 40-kDa linearpolyethylenimine (Polysciences, 24,765). Retrovirus generation was by using PLAT-A cells (Cell Biolab) ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were infiltrated with a 1:1 mixture of propylene oxide and Polybed 812 epoxy resin (Polysciences, Inc.) for 2 hours and infiltrated overnight ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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bioRxiv - Genetics 2024Quote: ... The enhancer luciferase reporter constructs with the control luciferase construct Renilla were co-transfected into cells using PEI (Polysciences). The luciferase signal was first normalized to that of the Renilla luciferase signal ...
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bioRxiv - Molecular Biology 2024Quote: HEK 293T cells were transfected with lentiviral constructs and packaging plasmids (psPAX2 and pMD2.G) using PEI transfection (Polysciences). Two days post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: The pcDNA3.4 plasmid containing His-tagged SARS-CoV-2 spike protein was transfected to Expi293T cells using PEI max (Polysciences). After 18–21 h post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-N1-RHBDL4 was transfected as a 1:10 mix with empty pcDNA3.1 plasmid into Hek293T cells (ATTC) using 25-kDa linear polyethylenimine (Polysciences) as had been described (Knopf et al ...
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bioRxiv - Cell Biology 2024Quote: ... 15μg of the MigR1-OsTIR2-TagBFP construct and 15μg of pCL-Eco packaging plasmid was co-transfected into HEK293T cells using PEI (Cat#23966, Polysciences). 48 and 72 hours later ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2020Quote: Cells at 30-40% confluency were transfected with 2µg DNA in six well plates using polyethylenimine (PEI; #23966, Polysciences Inc.). pcDNA3.1 or pBlueScript were used as empty vector controls ...
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bioRxiv - Immunology 2022Quote: ... The S-2P and S-6P plasmids were transiently transfected into HEK293F cells using 25-kDa linear polyethyleneimine (PEI) (Polysciences) with the PEI:DNA mass ratio of 3:1 and 1 mg DNA for per liter of culture when the cell density reached 2 × 106 cells per mL ...
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bioRxiv - Molecular Biology 2021Quote: Retrovirus packaging cells were transfected with pQCXIP-hMETTL18-HA or pQCXIP-hMETTL18-Asp193Lys-Gly195Arg-Gly197Arg-HA using PEI transfection reagent (Polysciences) and cultured for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 500 ng of GFP-LRRK2 construct and 500 ng of GFP-Rab10 using polyethylenimine (PEI, Polysciences). After 36 h ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: ... Env plasmids were co-transfected in Human Embryonic Kidney 293T or GnT1-293S cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid (23 ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Biochemistry 2021Quote: ... and wildtype b0,+AT (the pEZT-C-TwinStrep-mCherry-OaSLC7A9 plasmid) were transfected into HeLa cells at 1:1 molar ratio by using PEI MAX (40,000 MW Linear; Polysciences, Inc.). The ratio of DNA ...
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bioRxiv - Biochemistry 2020Quote: Plasmids encoding human EMC4 with an N- or C-terminal 3xHA tag in the pcDNA5/FRT/TO vector were transiently transfected into HEK293T cells using PEI “MAX” (Polysciences). At 3 days after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... All protein encoding sequences were subcloned to pCAG vector with N-terminal FLAG-tag for expression in HEK293F cell lines transfected by polyethylenimines (PEIs, Polysciences). Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with FACS buffer and fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Warrington, PA) diluted in FACS buffer (fixation buffer) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the constructed plasmid using polyethyleneimine (Polysciences). Transfected cells were imaged using a IX83 wide-field fluorescence microscopy (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Purified cDNA was incorporated into a pcDNA3.1 vector and transfected into HEK293T cells using PEI max transfection reagent (Polysciences, Germany) as described previously (18) ...
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bioRxiv - Cell Biology 2020Quote: ... Each plasmid and packaging vectors (pCMV-VSV-G-RSV-Rev and pCAG-HIVgp) were used to transfect HEK293T cells with Polyethylenimine MAX transfection reagent (Polysciences), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: Subconfluent 293T cells in a T75 flask were transfected with 15 μg of the SARS-CoV-2 S plasmid using polyethylenimine (Polysciences), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...