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Citations for Polysciences :
501 - 550 of 745 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: N2A-Pz1-KO cells were transfected using PEI-Max (Polysciences Inc, Cat. Nu. 24765-100) with 1500 ng ptdTomato-N1 vector ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... or pMSCV-puro-based constructs were transiently transfected in HEK293T cells using polyethylenimine (Polysciences, 24765). For lentivirus production ...
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bioRxiv - Cell Biology 2024Quote: Equal numbers of HEK293T cells were seeded Wnt7a plasmid was transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with benzidine in combination with the Differential Quick Stain Kit (PolySciences, Warrington, PA) (manufacturers protocol) ...
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bioRxiv - Cancer Biology 2019Quote: Retrovirus was produced in Plat-E cells by transient transfection with PEI (Polysciences; Cat#23966-1) according to standard protocols ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The fixed and stained cells were washed with PBS and mounted using Aqua-Poly/Mount (Polysciences). Confocal images of infected cells were acquired using a Leica SP8 Scanning Confocal Microscope controlled by Leica-X software ...
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bioRxiv - Biochemistry 2022Quote: ... 30,000 cells per 48 well were plated and transfected with 1 μl PEI (Polysciences, PEI 25K), 75 ng of promoter reporter plasmids (3xMBS-pGL4.10 or pGl4.10 empty vector ...
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bioRxiv - Microbiology 2022Quote: ... or the SLC35A1-targeting plasmid to generate SLC35A1KO cells using polyethylenimine (PEI; Polysciences, Warrington, PA, USA). At 24 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were dehydrated with increasing amounts of ethanol and embedded in epon epoxy resin (Polysciences). Ultrathin sections of 60 nm were contrasted with uranyl acetate and lead citrate using an AC20 automatic contrasting system (Leica ...
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bioRxiv - Immunology 2020Quote: ... The vector with antigen-bearing component was transfected into FreeStyle 293F cells using polyethylenimine (Polysciences, Inc) as described previously 7 ...
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bioRxiv - Biochemistry 2020Quote: ... The expression construct was transiently transfected in HEK 293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained in FreeStyle 293 medium and transfected using the PEI Max reagent (Polysciences Inc.) with a 1:3 ratio (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Each expression vector was transiently transfected into Expi293 cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2021Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 1.25 μg/well of the reporter plasmids by polyethyleneimine reagent (Polysciences, #23966) and differentiated in DMEM/10% FBS with 1µM rosiglitazone for two days ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed freshly prepared mixture of 1% glutaraldehyde (Polysciences Inc, Warrington, PA) and 1% osmium tetroxide (Polysciences Inc. ...
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bioRxiv - Immunology 2022Quote: ... cultures of FreeStyle 293 (Thermo) cells were transiently transfected with the constructs using PEI MAX (Polysciences) at a 1:4 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with AAV plasmid and helper plasmid using PEI Max 40000 (PolySciences, USA). Twenty-four hours later ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... SV589 cells grown on glass coverslips were transfected with pEGFP-GM130 FL using PEI Max (Polysciences) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was transfected into 293T cells using PEI (Polysciences, high potency linear, MW = 40 000) transfection reagent ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (ATCC, Manassas, USA) were transfected with pNL-EGFP/pVSV using polyethylenimine (PEI; Polysciences, Germany) (PEI/DNA 6/1 ratio ...
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bioRxiv - Immunology 2023Quote: Raw264.7 cells in a confluent 15cm dish were incubated with ∼108 magnetic beads (1μm size, Polysciences) for 4h ...
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bioRxiv - Cell Biology 2023Quote: Immunogold electron microscopy was performed on thin sections of cells embedded in Lowicryl K4M (Polysciences Inc.). HAP1 WT or mini-NEAT1 cells fixed in situ for 1 h at 4°C with 4% formaldehyde in 0.1 M Sörensen phosphate buffer pH 7.3 were scraped off and centrifuged ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were transfected with GPΔmuc and GPΔmucHRV3C plasmid using polyethyleneimine (PEI MAX, Polysciences, Warrington, PA) at a mass ratio of 1:3 PEI MAX:DNA ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, 111-30-8) in 0.01 M PBS (Fisher) ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Adeno-associated viruses (AAVs) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Plasmid transfection of 293T and HeLa cells was performed using polyethyleneimine (Polysciences Inc., Warrington, PA, USA) and HilyMax (DOJINDO Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... The Ecad and Dsc3 plasmids were transiently transfected into HEK 293T cells using Polyethylenimine (PEI) (Polysciences, Inc.). Four days post transfection ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were plated in two 10 cm2 dishes and transfected using PEI MAX (Polysciences, PA USA) with vRNP expression plasmids (3 µg/mL each of pCAGGS/PB2-FLAG ...
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bioRxiv - Neuroscience 2021Quote: ... The cells in each well were transfected with 1.6 μg PEI (Polyethylenimine, Linear, MW 25000, Polysciences, U.S.A.) and 400 ng of pMU2-Camk2b plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus was produced by transient transfection of HEK293T cells (ATCC) using linear 25 kDa polyethyleneimine (PEI; Polysciences). Briefly ...
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bioRxiv - Immunology 2021Quote: rAAV-DJ were produced in HEK293T cells (ATCC) by triple transient transfection using polyethylenimine (PEI, Polysciences Inc). For each vector ...
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bioRxiv - Microbiology 2020Quote: ... triple plasmid transfection of 293 adherent cells was performed with a polyethylenimine (PEI)-MAX (Polysciences, MW, 40,000) to DNA ratio of 3:1 (a mass-per-mass ratio) ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was added at a final concentration of 0.675 μg/mio cells and polyethylenimine (Polysciences, #23966-1) was added to a final concentration of 0.01 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... plasmids encoding DISP1-A-NNN-SBP were transfected into the HEK293F cells using linear polyethylenimines (PEIs, Polysciences). Both DISP1-A and DISP1-A-NNN were purified essentially as previously described[17] ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were incubated using 1 mg/mL of Polyethylenimine (PEI, Linear, MW 25000, Polysciences, PA, USA) at a ratio of 1:6 (DNA:PEI) ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were transiently transfected with appropriate cDNA using PEI (Polyethylenimine, linear, MW 25000; Polysciences, Inc).
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bioRxiv - Biochemistry 2020Quote: ... HDX-MS and MALS analysis were expressed in HEK293-GnT1 suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...