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Citations for Polysciences :
451 - 500 of 620 citations for Hydrotris pyrazol 1 ylborato trichlorotitanium IV since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and sorting efficiency was verified manually by sorting a specified number of 1-μm yellow-green microspheres (Polysciences, USA) onto a glass slide and counting the beads under an epifluorescence microscope ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...
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bioRxiv - Microbiology 2020Quote: ... was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear, MW 25,000, 23966-1-A, Polysciences) transfection method (45) ...
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bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Biophysics 2022Quote: ... The cells in each well were transiently transfected with 1 μg HcKCR1/2-CGFP DNA plasmid using 3μg linear polyethylenimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
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bioRxiv - Cell Biology 2022Quote: ... PRMT6-HA or Pu.1 as indicated using Polyethylenimine according to the manufacturer’s protocol (PEI, Polysciences, Cat#24765-2)
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... or with GFP-tau and vectors pCDNA3 overexpressing Cdk5 and p25 (1:0.85:0.15) using linear Polyethylenimine (PEI; Polysciences, Inc.). Cells were harvested 48 hours after transfection by centrifugation and flash-frozen ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was carried out by placing adult worms in 1.5 μl of M9 mixed with 1.5 μl of 1 μm polysterene microspheres (Polysciences Inc.) on a coverslip which was mounted carefully onto a 5% agarose pad.
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... the left sides of the brains were placed (anterior portion facing downwards) in disposable cryomold (Polysciences Inc. #18986– 1), and frozen in O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Bioengineering 2024Quote: ... 8.3 µg psPAX and 2.5 µg pMD2.G were mixed with 80 µg PEI MAX 40K (Polysciences, 24765–1) in 1.5 ml of Expi293 media ...
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bioRxiv - Neuroscience 2024Quote: ... Cells at ∼80% confluency were transfected with DNA plasmids using polyethyleneimine max according to the manufacturer’s manual (PEI Max; 24765-1, Polysciences). For CaST experiments ...
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bioRxiv - Bioengineering 2024Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed EV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5-1 μg/mL of each construct was transfected with 5 μg/mL polyethyleneimine (PEI-25K; Polysciences, 23966). 24 hr after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-N1-RHBDL4 was transfected as a 1:10 mix with empty pcDNA3.1 plasmid into Hek293T cells (ATTC) using 25-kDa linear polyethylenimine (Polysciences) as had been described (Knopf et al ...
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bioRxiv - Developmental Biology 2024Quote: ... A second droplet of (∼ 10 µl) of a solution of 1 µm polystyrene microspheres (Polysciences, cat. no. 07310-15) was then added and gently mixed ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... MLV-GagPol/pHIV 8.1 and pHIT VSVg at a ratio of 2:3:1 for 48 h using polyethylenimine (Polysciences, Germany). 293T supernatant was collected and filtered through a 0.2-μm filter before being used to transduce MDCK II cells ...
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bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Microbiology 2021Quote: ... the construct was expressed in 293F by transfecting with PEIMAX PEI MAX™ (24765-1, Polysciences, Inc., Warrington, PA, USA) according to the manual ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
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bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenized mixture was then poured into a solution containing 1% polyvinyl alcohol (25 kDa, 88% hydrolys, Polysciences, Warrington, PA) in water under continuous stirring ...
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bioRxiv - Cell Biology 2020Quote: HEK293T stable cell lines for HSPB8-V5 were transiently transfected with different wild type or mutant BAG3-GFP constructs using PEI (23966-1, PolySciences Europe ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...