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Citations for ApexBio :
1 - 50 of 53 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on a 10% SDS gel containing 20 μM Phos-tag acrylamide (ApexBio) and 100 µM MnCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... containing protease inhibitor (ApexBio Technology ...
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bioRxiv - Cell Biology 2022Quote: ... RIPA buffer containing FLAG peptide (ApexBio) by incubating on ice for 5 min ...
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bioRxiv - Immunology 2022Quote: ... containing 160µg/mL 1xFLAG peptide (ApexBio) pH 7.5 following a 1-hour incubation with mild agitation ...
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bioRxiv - Physiology 2020Quote: ... containing protease inhibitor (Mammalian ProteaseArrest-APExBIO K1008). The tissue was then vortexed and incubated on ice for 30 min with occasional further disruption with surgical scissors ...
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bioRxiv - Microbiology 2020Quote: ... 0.5%sodium deoxycholate containing 1×Protease Inhibitor Cocktail (APeXBio Technology ...
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bioRxiv - Biochemistry 2023Quote: ... We added 0.5 mL of solution containing 2× APEX2/BxxT (APExBIO) in PBS (U2OS in 3.5 cm dish ...
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bioRxiv - Plant Biology 2023Quote: ... The purified protein was digested with PreScission Protease (APEXBIO) to remove GST tag and treated with 25 mM DTT or 50 μM H2O2 at 25 °C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1x Laemmli Buffer containing 1x Protease Inhibitor Cocktail (APExBio, Cat. # K1007) and 1x Phosphatase Inhibitor Cocktail 1 (APExBio ...
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bioRxiv - Cell Biology 2021Quote: ... samples were run on a 6% SDS-PAGE gel containing 100 μM Phosbind (Apexbio) according to the manufacturer’s protocol to separate phosphorylated isoforms of Sts5 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pellet was resuspended in RIPA buffer containing both protease inhibitors (ApexBio®) and tyrosine-phosphatase inhibitors (Sigma® ...
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bioRxiv - Pathology 2023Quote: ... and half of the wells were switched to HBSS containing 10μm forskolin (Apexbio, B1421). Organoids were imaged every 10 m after initial switching to HBSS by using a BZ-X810 Microscope (Keyence ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 M sucrose) containing 1x Protease Inhibitor and 1x Phosphatase Inhibitor Cocktail I (ApexBio). Cells were allowed to swell for 10 min on ice and then homogenized with 30 strokes of a glass Dounce homogenizer ...
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bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... at a density of 300,000 cells per well in mTeSR1 medium containing 10 µM Y27632 (APExBIO) (Day -4) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed with PBS and then incubated with HBSS containing 0.5mM biotin phenol (APExBIO A8011) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in cold DMEM-F12 w/HEPES containing 10 µM Y27632 (APExBIO, USA) and the resulting suspension was filtered through a 70-µm nylon mesh and centrifuged at 1,000 x g for 5 min ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were rested in SFEM II containing StemSpan cytokines and 5 µM Z-VAD-FMK (ApexBio) for 72 hours and then directed to granulocytic differentiation as described above ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved by SDS-PAGE and stained with InstaBlue (APExBIO). The intensity of gel bands was quantified with ImageJ (38 ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lamin and GST-dKeap1 proteins were treated with PreScission Protease (APExBIO). GST pull downs were conducted using Glutathione Sepharose 4B beads (Cytiva 17075601 ...
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bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
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bioRxiv - Molecular Biology 2023Quote: ... Flag-TOE1 was eluted with 100 µl of NET-2 containing 150 ng/µl FLAG peptide (ApexBio) and 10% glycerol by rotating at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Resin was washed again with 3 bed volumes of buffer and then eluted in 5×0.5 bed volumes of buffer containing 0.25 mg/mL 3×FLAG peptide (APExBio). The protein was further purified by gel filtration (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2024Quote: ... pH 6.5 prior to competitive elution with ∼2.5 column volumes of buffer containing 0.3 mg/mL of DYKDDDK tag peptide (A6002, APExBIO). The eluted protein was concentrated on a 10 kDa MWCO PES membrane Vivaspin 6 Centrifugal Concentrator (Sartoris ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with Neurobasal media containing 5 μM of kinase inhibitor from the DiscoveryProbeTM Kinase Inhibitor Library (APExBIO). The following inhibitors were used ...
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bioRxiv - Bioengineering 2023Quote: ... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... N-2a cells were cultured for 1 hour in DMEM (+) media containing kinase inhibitors from the DiscoveryProbeTM Kinase Inhibitor Library (APExBIO). After 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Dolastatin-induced tubulin-rings (D-rings) were polymerized by diluting tubulin to 40 μM in BRB80 buffer containing 80 μM dolastatin-10 (APExBIO) and incubating the reaction for 1 hour at 25 °C ...
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bioRxiv - Cell Biology 2023Quote: ... APEX labelling was initiated by replacing the medium with fresh medium containing 500 μM biotin-phenol (APExBIO, cat#A8011-1000) and incubated in 5% CO2 at 37 ℃ for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein samples were resolved by SDS-PAGE with or without 50 μM phos-tag (ApexBio F4002). OsBZR1 was detected by the commercial anti-OsBZR1 and anti-HSP was used as equal loading control (BPI ...
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bioRxiv - Physiology 2023Quote: ... Freshly isolated SAN cells were put into 35 mm tissue culture plates with Media 199 containing Blebbistatin 10 mM (ApexBio B1387-10), 5% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with buffer A supplemented with 0.2 mg/ml 3× FLAG peptide (APExBio A6001), concentrated in a 30-kD MWCO Amicon centrifugal filtration device ...
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bioRxiv - Biochemistry 2022Quote: ... and the proteins were eluted by incubating the beads with 200 μg/mL 3xFLAG peptide (APExBIO, A6001) for 2 hours at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was eluted with buffer A supplemented with 0.25 mg/ml 3× FLAG peptide (A6001; APExBio) for biochemical assays ...
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bioRxiv - Immunology 2023Quote: ... Cy5 Goat Anti-Rabbit IgG (H+L) (K1212) secondary antibodies (APExBIO); anti-HA (66006-2-Ig) ...
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bioRxiv - Biochemistry 2023Quote: ... Oocytes expressing paddle chimera or designed channels were collected and lyzed using Tris buffer (pH 8) containing protease inhibitors (APExBIO, catalog No. K1007). The lyzed oocytes were manually homogenized and centrifuged at 1500 g for 15 minutes ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... we incubated the monoclonal Dynabeads MyOne Streptavidin T1 (Thermo) was incubated with purified protein diluent and biotin (APExBIO) or biotin-poly (I:C ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 5 CV of purification buffer supplemented with 150 μg/mL 3X-FLAG peptide (Apexbio). Fractions containing NavPas were pooled and concentrated ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed with PBS and pre-treated with 5 µmol L-1 inhibitor of apoptosis protein antagonist BV6 (APExBIO) and 20 µmol L-1 pan-caspase-inhibitor zVAD-fmk (Bachem ...
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bioRxiv - Cancer Biology 2019Quote: ... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Culture media was washed from cells twice with 1x PBS and then cell surface exposed proteins were labeled with sulfo-NHS-SS-biotin (APExBio) at a final concentration of 1 mg/mL in 1x PBS at 4℃ for 20 minutes ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: ... Protein was eluted twice from the resin by incubating with 300 µL of 500 µg/mL 3X Flag Peptide (APExBIO, A6001) rotating at 4 °C for 20 minutes ...