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Citations for ApexBio :
1 - 50 of 96 citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... We added 0.5 mL of solution containing 2× APEX2/BxxT (APExBIO) in PBS (U2OS in 3.5 cm dish ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on a 10% SDS gel containing 20 μM Phos-tag acrylamide (ApexBio) and 100 µM MnCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-TOE1 was eluted with 100 µl of NET-2 containing 150 ng/µl FLAG peptide (ApexBio) and 10% glycerol by rotating at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... containing protease inhibitor (ApexBio Technology ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... RIPA buffer containing FLAG peptide (ApexBio) by incubating on ice for 5 min ...
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bioRxiv - Immunology 2022Quote: ... containing 160µg/mL 1xFLAG peptide (ApexBio) pH 7.5 following a 1-hour incubation with mild agitation ...
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bioRxiv - Physiology 2020Quote: ... containing protease inhibitor (Mammalian ProteaseArrest-APExBIO K1008). The tissue was then vortexed and incubated on ice for 30 min with occasional further disruption with surgical scissors ...
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bioRxiv - Microbiology 2020Quote: ... 0.5%sodium deoxycholate containing 1×Protease Inhibitor Cocktail (APeXBio Technology ...
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bioRxiv - Biochemistry 2023Quote: ... The second round of PCR (8-9 cycles) added i5 and i7 indexes using the Index Kit 2 for Illumina (Apexbio Technology LLC). The libraries were cleaned up using AMPure XP beads ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
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bioRxiv - Cell Biology 2023Quote: ... 6 mM of 2-deoxyglucose (Apexbio) and 10 mM of sodium azide (G-Biosciences ...
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bioRxiv - Plant Biology 2023Quote: ... The purified protein was digested with PreScission Protease (APEXBIO) to remove GST tag and treated with 25 mM DTT or 50 μM H2O2 at 25 °C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1x Laemmli Buffer containing 1x Protease Inhibitor Cocktail (APExBio, Cat. # K1007) and 1x Phosphatase Inhibitor Cocktail 1 (APExBio ...
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bioRxiv - Cell Biology 2021Quote: ... samples were run on a 6% SDS-PAGE gel containing 100 μM Phosbind (Apexbio) according to the manufacturer’s protocol to separate phosphorylated isoforms of Sts5 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pellet was resuspended in RIPA buffer containing both protease inhibitors (ApexBio®) and tyrosine-phosphatase inhibitors (Sigma® ...
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bioRxiv - Pathology 2023Quote: ... and half of the wells were switched to HBSS containing 10μm forskolin (Apexbio, B1421). Organoids were imaged every 10 m after initial switching to HBSS by using a BZ-X810 Microscope (Keyence ...
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bioRxiv - Cell Biology 2021Quote: Akt1/2/3 inhibitor MK-2206 dihydrochloride (ApexBio, A3010), Rapamycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (APExBIO, Cat. No. B8337) was injected intraperitoneally at a concentration of 50 mg/kg body weight and mice were euthanized by cervical dislocation 12 or 24 h later.
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bioRxiv - Neuroscience 2023Quote: ... The small molecule inhibitors rimonabant (ApexBio, Lot #B1429-2) and istradefylline (Tokyo Chemical Industry ...
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bioRxiv - Cancer Biology 2023Quote: ... The IKKβ inhibitor IKK-2 inhibitor VIII (ApexBio, #A3485), puromycin dihydrochloride (Thermo Fisher #A1113803) ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 M sucrose) containing 1x Protease Inhibitor and 1x Phosphatase Inhibitor Cocktail I (ApexBio). Cells were allowed to swell for 10 min on ice and then homogenized with 30 strokes of a glass Dounce homogenizer ...
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bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... 2% BSA and 10 µM Y-27632 (APExBIO, Cat#3008) and then filtered through a 30 µM mesh ...
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bioRxiv - Cell Biology 2020Quote: ... at a density of 300,000 cells per well in mTeSR1 medium containing 10 µM Y27632 (APExBIO) (Day -4) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed with PBS and then incubated with HBSS containing 0.5mM biotin phenol (APExBIO A8011) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in cold DMEM-F12 w/HEPES containing 10 µM Y27632 (APExBIO, USA) and the resulting suspension was filtered through a 70-µm nylon mesh and centrifuged at 1,000 x g for 5 min ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were rested in SFEM II containing StemSpan cytokines and 5 µM Z-VAD-FMK (ApexBio) for 72 hours and then directed to granulocytic differentiation as described above ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved by SDS-PAGE and stained with InstaBlue (APExBIO). The intensity of gel bands was quantified with ImageJ (38 ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lamin and GST-dKeap1 proteins were treated with PreScission Protease (APExBIO). GST pull downs were conducted using Glutathione Sepharose 4B beads (Cytiva 17075601 ...
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bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
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bioRxiv - Systems Biology 2021Quote: Caco-2 cells were pre-treated with Camostat Mesilate (ApexBio, B2082), Apilimod (Selleckchem ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 µM R428 (Tyrosine kinase receptor AXL inhibitor) (ApexBio, A8329). From d1 to d11 media was changed every day and from d12 to d27 media was changed every other day ...
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bioRxiv - Cell Biology 2021Quote: ... Resin was washed again with 3 bed volumes of buffer and then eluted in 5×0.5 bed volumes of buffer containing 0.25 mg/mL 3×FLAG peptide (APExBio). The protein was further purified by gel filtration (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2024Quote: ... pH 6.5 prior to competitive elution with ∼2.5 column volumes of buffer containing 0.3 mg/mL of DYKDDDK tag peptide (A6002, APExBIO). The eluted protein was concentrated on a 10 kDa MWCO PES membrane Vivaspin 6 Centrifugal Concentrator (Sartoris ...
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bioRxiv - Cell Biology 2024Quote: ... (2- (Dimethylamino)-N-(6-oxo-5,6-dihydrophenanthridin-2-yl)acetamide hydrochloride (PJ34; A41159, ApexBio) N-(p-amylcinnamoyl ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with Neurobasal media containing 5 μM of kinase inhibitor from the DiscoveryProbeTM Kinase Inhibitor Library (APExBIO). The following inhibitors were used ...
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bioRxiv - Biophysics 2022Quote: ... HMQC spectra were recorded with 0 and 2 mM gadodiamide (APExBIO, Houston, TX). These spectra were collected on an 850 MHz spectrometer ...
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bioRxiv - Bioengineering 2023Quote: ... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... N-2a cells were cultured for 1 hour in DMEM (+) media containing kinase inhibitors from the DiscoveryProbeTM Kinase Inhibitor Library (APExBIO). After 1 hour ...
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bioRxiv - Biochemistry 2022Quote: Dolastatin-induced tubulin-rings (D-rings) were polymerized by diluting tubulin to 40 μM in BRB80 buffer containing 80 μM dolastatin-10 (APExBIO) and incubating the reaction for 1 hour at 25 °C ...
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bioRxiv - Cell Biology 2023Quote: ... APEX labelling was initiated by replacing the medium with fresh medium containing 500 μM biotin-phenol (APExBIO, cat#A8011-1000) and incubated in 5% CO2 at 37 ℃ for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The CHEK1/2 inhibitors AZD7762 (Selleckchem, Houston, TX) and CHIR-124 (ApexBio, Houston, TX) were applied at 8 different concentrations between 10 µM and 4.6 nM (3-fold dilution steps) ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...