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Citations for ApexBio :
1 - 50 of 94 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 1 mM HPDP-biotin (A8008; APExBIO, Houston, TX), 0.2% TritonX-100 (T8787 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... we incubated the monoclonal Dynabeads MyOne Streptavidin T1 (Thermo) was incubated with purified protein diluent and biotin (APExBIO) or biotin-poly (I:C ...
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bioRxiv - Cell Biology 2023Quote: ... Culture media was washed from cells twice with 1x PBS and then cell surface exposed proteins were labeled with sulfo-NHS-SS-biotin (APExBio) at a final concentration of 1 mg/mL in 1x PBS at 4℃ for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30 minutes and biotin (APExBIO A8010) was added at 50 μM and incubated for another 2.5 hours ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated with 500 μM biotin-phenol (APExBIO) at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: We mixed 97 μL of 100 mM Tris-HCl (pH 7.5) buffer with 2 μL of APEX2 and 1 μL of 100 mM Biotin-tyramide (BT, APExBIO) in DMSO onto a 96-well microplate ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µM Aurora B inhibitor AZD1152 (ApexBio, A4112-APE-10mM), 25-100 nM PP1/PP2A inhibitor calyculin A (TOCRIS ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µM Aurora B inhibitor ZM 447439 (ApexBio, A4113-APE-10mM), 10 µM Aurora B inhibitor AZD1152 (ApexBio ...
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bioRxiv - Cell Biology 2020Quote: Cytosolic lysate was covalently modified with or without 1mg/mL sulfo-NHS-LC biotin (ApexBio) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were separated by 10% SDS–PAGE with 30 μM Phos-binding reagent acrylamide (APExBIO, F4002) and transferred onto PVDF membranes for immunoblotting analyses.
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed with PBS and then incubated with HBSS containing 0.5mM biotin phenol (APExBIO A8011) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... standard discontinuous 20 % polyacrylamide gels were prepared with the modification that 50 µM Phos binding reagent acrylamide (PBR-A, APExBIO) and 100 µM Zn(NO3)2 were added to the separating gel ...
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bioRxiv - Microbiology 2024Quote: ... The phosphorylation status of RsbV1/V2 was observed by running samples on SDS-PAGE gels (16% for His-V1/V2) supplemented with 20 µM of Phos binding reagent (APExBIO). Gels were stained with Coomassie brilliant blue and visualized with a Chemidoc Imager.
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed with the Schneider’s medium to remove fat bodies and debris and were incubated in 100 μM of biotin-phenol (BP; APExBIO) in the Schneider’s medium on ice for one hour ...
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bioRxiv - Bioengineering 2022Quote: ... Biotinylation of the gelatin-layer formed in the nanovial cavity was conducted by incubating nanovials with 10 mM Sulfo-NHS-Biotin (APExBIO) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylation of the gelatin-layer formed in the nanovial cavity was conducted by incubating nanovials with 10 mM Sulfo-NHS-Biotin (APExBIO) overnight at room temperature while mixing ...
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bioRxiv - Cell Biology 2023Quote: ... intact cells were washed twice with ice-cold DPBS and incubated with the membrane-impermeable biotinylation reagent Sulfo-NHS SS-Biotin (0.5 mg ⁄ mL; APExBIO, #A8005) in DPBS containing 0.1 mM CaCl2 and 1 mM MgCl2 (PBS+CM ...
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bioRxiv - Cell Biology 2023Quote: ... APEX labelling was initiated by replacing the medium with fresh medium containing 500 μM biotin-phenol (APExBIO, cat#A8011-1000) and incubated in 5% CO2 at 37 ℃ for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The intact cells were washed one time with ice cold DPBS and incubated with the membrane impermeable biotinylation reagent Sulfo-NHS SS-Biotin (0.5 mg/mL; APExBio, catalog #: A8005) in PBS containing 0.1 mM CaCl2 and 1 mM MgCl2 (PBS+CM ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were briefly washed with cold PBS prior to incubation with 2 mg/mL Sulfo-NHS-SS-Biotin (APExBIO cat. # A8005) in cold PBS for thirty minutes on ice ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed with PBS and pre-treated with 5 µmol L-1 inhibitor of apoptosis protein antagonist BV6 (APExBIO) and 20 µmol L-1 pan-caspase-inhibitor zVAD-fmk (Bachem ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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bioRxiv - Plant Biology 2023Quote: ... The purified protein was digested with PreScission Protease (APEXBIO) to remove GST tag and treated with 25 mM DTT or 50 μM H2O2 at 25 °C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved by SDS-PAGE and stained with InstaBlue (APExBIO). The intensity of gel bands was quantified with ImageJ (38 ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lamin and GST-dKeap1 proteins were treated with PreScission Protease (APExBIO). GST pull downs were conducted using Glutathione Sepharose 4B beads (Cytiva 17075601 ...
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bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on a 10% SDS gel containing 20 μM Phos-tag acrylamide (ApexBio) and 100 µM MnCl2 ...
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bioRxiv - Bioengineering 2023Quote: ... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein samples were resolved by SDS-PAGE with or without 50 μM phos-tag (ApexBio F4002). OsBZR1 was detected by the commercial anti-OsBZR1 and anti-HSP was used as equal loading control (BPI ...
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bioRxiv - Cancer Biology 2022Quote: The cuproptosis inducer Elesclomol+Cu2+ (1:1 ratio, APExBIO) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with buffer A supplemented with 0.2 mg/ml 3× FLAG peptide (APExBio A6001), concentrated in a 30-kD MWCO Amicon centrifugal filtration device ...
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bioRxiv - Biochemistry 2022Quote: ... and the proteins were eluted by incubating the beads with 200 μg/mL 3xFLAG peptide (APExBIO, A6001) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was eluted with buffer A supplemented with 0.25 mg/ml 3× FLAG peptide (A6001; APExBio) for biochemical assays ...
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bioRxiv - Immunology 2023Quote: ... Cy5 Goat Anti-Rabbit IgG (H+L) (K1212) secondary antibodies (APExBIO); anti-HA (66006-2-Ig) ...
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bioRxiv - Cell Biology 2020Quote: ... BML-210 and stemregenin 1 (SR-1) were from ApexBio Technology LCC (Houston ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... BML-210 and stemregenin 1 (SR-1) were from ApexBio Technology LCC (Houston ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT and 1 mM complete protease inhibitors (APExBIO) at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 5 CV of purification buffer supplemented with 150 μg/mL 3X-FLAG peptide (Apexbio). Fractions containing NavPas were pooled and concentrated ...
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bioRxiv - Cancer Biology 2019Quote: ... Nec-1 (ApexBio, USA), cisplatin (ApexBio ...
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bioRxiv - Neuroscience 2021Quote: ... TAPI-1 (ApexBio; B4686), Verubecestat (ApexBio ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... sephin 1 (Apexbio,A8708) and CCCP (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µM BIRB796 (APExBIO), 3 µM CHIR99021 ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...